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Immunohistochemical Diagnostic value of Calretinin in Hirschprung Disease

Nusantara Medical Science Journal · Vol. ume (2023) · pp. 27–35

DOI
10.20956/nmsj.vi.20000
ISSN
2597-7288, 2460-9757
Published
2023-11-02
Publisher
Faculty of Medicine, Hasanuddin University.
Language
en
License
creativecommons.org/licenses/by/4.0

Abstract

Introduction: Hirschsprung's disease (HD) is characterized by the absence of ganglion cells in Meissner's plexus and Auerbach's plexus which used to be assessed by histopathological examination using Hematoxylin and Eosin (H&E). In some cases is difficult to assess ganglion cells, thus requiring additional examination in the form of calretinin immunohistochemistry to detect the presence of ganglion. Methods: The 59 samples of suspected HD were analyzed by H&E staining followed by calretinin immunohistochemical staining. This study aimed to assess the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of calretinin in HD diagnostics. Results: The immunohistochemistry of calretinin showed a good diagnostic value in detecting aganglionosis in patients with suspected HD with a sensitivity value = 97.7%; Specificity = 100%; Positive Presumptive Value (PPV) = 100%; Negative Presumptive Value (NPV) = 93.7%; Accuracy= 98.3%. Thus, calretinin immunohistochemistry can be relied upon as an additional method in the diagnosis of HD

Collaboration network of the authors

30 authors · 89 co-authorship links

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  • Hasanuddin University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Cahyono KaelanAffiliation unknown1
Rina MasadahAffiliation unknown1
Jane Magdalena KalatikuAffiliation unknown1
Berti J NelwanAffiliation unknown1
Djumadi AchmadAffiliation unknown1
Firdaus HamidAffiliation unknown1
Erwin ChandraAffiliation unknown1
Sulmiati SulmiatiAffiliation unknown1
Fonny JoshAffiliation unknown1
Sachraswaty RachmanAffiliation unknown1
Prihantono PrihantonoAffiliation unknown1
Aussie Fitriani GhaznawieAffiliation unknown1
Akhtar Fajar MuzakkirAffiliation unknown1
F. FathlinaAffiliation unknown1
Ali Aspar MappahyaAffiliation unknown1
Abdul Hakim AlkatiriAffiliation unknown1
Idar MappangaraHasanuddin University1
Zaenab DjafarAffiliation unknown1
Muzakkir AmirAffiliation unknown1
Andi Muhammad Akram KastiranAffiliation unknown1
Melda WarlianiAffiliation unknown1
Rini Rachmawarni BachtiarAffiliation unknown1
Rahmawati Minhajat1Affiliation unknown1
Sri JayantiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/jmednus/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Immunohistochemical Diagnostic value of Calretinin in Hirschprung Disease</dc:title>
	<dc:creator xml:lang="en">Azis, Zainab</dc:creator>
	<dc:creator xml:lang="en">Cangara, M. Husni </dc:creator>
	<dc:creator xml:lang="en">Zainuddin, Andi Alfian</dc:creator>
	<dc:creator xml:lang="en">Wahid, Syarifuddin </dc:creator>
	<dc:creator xml:lang="en">Mariana, Nita </dc:creator>
	<dc:creator xml:lang="en">Miskad, Upik A. </dc:creator>
	<dc:description xml:lang="en">



Introduction: Hirschsprung's disease (HD) is characterized by the absence of ganglion cells in Meissner's plexus and Auerbach's plexus which used to be assessed by histopathological examination using Hematoxylin and Eosin (H&amp;amp;E).  In some cases is difficult to assess ganglion cells, thus requiring additional examination in the form of calretinin immunohistochemistry to detect the presence of ganglion. Methods: The 59 samples of suspected HD  were analyzed by H&amp;amp;E staining followed by calretinin immunohistochemical staining. This study aimed to assess the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of calretinin in HD diagnostics. Results:  The immunohistochemistry of calretinin showed a good diagnostic value in detecting aganglionosis in patients with suspected HD with a sensitivity value = 97.7%; Specificity = 100%; Positive Presumptive Value (PPV) = 100%; Negative Presumptive Value (NPV) = 93.7%; Accuracy= 98.3%. Thus, calretinin immunohistochemistry can be relied upon as an additional method in the diagnosis of HD



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	<dc:publisher xml:lang="en">Faculty of Medicine, Hasanuddin University.</dc:publisher>
	<dc:date>2023-11-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/jmednus/article/view/20000</dc:identifier>
	<dc:identifier>10.20956/nmsj.vi.20000</dc:identifier>
	<dc:source xml:lang="en">Nusantara Medical Science Journal; Volume 8 Issue 2, July - December 2023; 27 - 35</dc:source>
	<dc:source>2597-7288</dc:source>
	<dc:source>2460-9757</dc:source>
	<dc:source>10.20956/nmsj.vi</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/jmednus/article/view/20000/12117</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Zainab Azis, M. Husni  Cangara, Andi Alfian Zainuddin, Syarifuddin  Wahid, Nita  Mariana, Upik A.  Miskad</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
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