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ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus

Torani Journal of Fisheries and Marine Science · Vol. 24 No. 2 (2016)

DOI
10.35911/torani.v24i2.227
ISSN
2615-6601, 2621-5322
Published
2016-05-19
Publisher
Hasanuddin University
Language
en

Abstract

The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032. Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD.

Collaboration network of the authors

11 authors · 20 co-authorship links

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  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
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  • Same organisation
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Co-authorOrganisationJoint articles
Hamka HamkaAffiliation unknown2
Asmi Citra Malina A.R TassakkaAffiliation unknown2
Andi Detti YuniantiAffiliation unknown1
Sitti NuraeniAffiliation unknown1
. SuhasmanAffiliation unknown1
Sahabuddin SahabuddinInstitut Agama Islam Negeri Kendari1
Iqbal BurhanuddinAffiliation unknown1
Nurhapsa NurhapsaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/torani/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">ANALISIS  KERAGAMAN  GENETIK PADA IKAN  KERAPU  MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP  PENYAKIT YANG   DISEBABKAN BAKTERI Vibrio alginolyticus</dc:title>
	<dc:creator xml:lang="en">Triana, St. Hidayah</dc:creator>
	<dc:creator xml:lang="en">Gani, Mahir S.</dc:creator>
	<dc:creator xml:lang="en">Malina, Asmi Citra</dc:creator>
	<dc:description xml:lang="en">The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032. Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2016-05-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/torani/article/view/227</dc:identifier>
	<dc:identifier>10.35911/torani.v24i2.227</dc:identifier>
	<dc:source xml:lang="en">Torani Journal of Fisheries and Marine Science; Vol. 24 No. 2 (2014)</dc:source>
	<dc:source>2615-6601</dc:source>
	<dc:source>2621-5322</dc:source>
	<dc:source>10.35911/torani.v24i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/torani/article/view/227/124</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Torani Journal of Fisheries and Marine Science</dc:rights>
</oai_dc:dc>