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Formulation and Test of Antioxidant Activity of Serum Active Fraction of Red Pomegranate Skin Extract (Punica granatum L.) by FRAP Method (Ferric Reducing Antioxidant Power)

Journal of Science and Technology Research for Pharmacy · Vol. 5 No. 1 (2025) · pp. 40–51

DOI
10.15294/jstrp.v5i1.10664
ISSN
2776-0685
Published
2025-07-25
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Aging of the body occurs with age, which can be seen in the skin. Skin aging is a physiological process that occurs in the human body. Free radicals are one of the causes of skin aging. By using antioxidant compounds, you can stop the increase in free radicals. Red pomegranate peel extract (Punica granatum L.) is one of the natural ingredients for a natural antioxidant, cause it’s contains polyphenols such as tannins. The aim of this research was to determine the fraction of pomegranate peel extract with the highest antioxidant activity and to determine the optimal serum formula for the red pomegranate peel fraction. The antioxidant activity testing method used in this research is the FRAP method (ferric reducing antioxidant power). The sample extract is fractionated, and then the fraction that has the highest antioxidant activity is made into a serum preparation formulation and preparation evaluation test. Based on the results of total tannin levels in all samples also showed positive results with a change in the color of the solution to bluish. The results of the antioxidant activity of all samples were categorized as very strong antioxidants as stated in the IC value50 < 50 ppm. Optimizing the antioxidant serum formulation used HPMC concentrations of 0.5%, 1% and 2% respectively. The results obtained from testing antioxidant activity obtained IC values50 the best was 0.368 ppm for the ethyl acetate fraction of red pomegranate peel. Optimizing the serum formula used a HPMC concentration of 1%, with an IC value50 amounting to 133,903 ppm.

Keywords

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10 authors · 15 co-authorship links

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Co-authorOrganisationJoint articles
K Syifa FauziyahAffiliation unknown1
Tristania Magdalena DumatubunAffiliation unknown1
Nabila Meilani DewiAffiliation unknown1
Arneta EvaniaAffiliation unknown1
R. SusantiAffiliation unknown1
Wulan ChristijantiAffiliation unknown1
Kartika WidyaningrumAffiliation unknown1
Mohd Salim Bin MohamedAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/JSTRP/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Formulation and Test of Antioxidant Activity of Serum Active Fraction of Red Pomegranate Skin Extract (Punica granatum L.) by FRAP Method (Ferric Reducing Antioxidant Power)</dc:title>
	<dc:creator xml:lang="en">Armandoko, Thianika Shalsabillah</dc:creator>
	<dc:creator xml:lang="en">Rakainsa, Senda Kartika</dc:creator>
	<dc:subject xml:lang="en">Red pomegranate peel extract</dc:subject>
	<dc:subject xml:lang="en">antioxidant</dc:subject>
	<dc:subject xml:lang="en">FRAP</dc:subject>
	<dc:subject xml:lang="en">fraction</dc:subject>
	<dc:subject xml:lang="en">serum</dc:subject>
	<dc:description xml:lang="en">Aging of the body occurs with age, which can be seen in the skin. Skin aging is a physiological process that occurs in the human body. Free radicals are one of the causes of skin aging. By using antioxidant compounds, you can stop the increase in free radicals. Red pomegranate peel extract (Punica granatum L.) is one of the natural ingredients for a natural antioxidant, cause it’s contains polyphenols such as tannins. The aim of this research was to determine the fraction of pomegranate peel extract with the highest antioxidant activity and to determine the optimal serum formula for the red pomegranate peel fraction. The antioxidant activity testing method used in this research is the FRAP method (ferric reducing antioxidant power). The sample extract is fractionated, and then the fraction that has the highest antioxidant activity is made into a serum preparation formulation and preparation evaluation test. Based on the results of total tannin levels in all samples also showed positive results with a change in the color of the solution to bluish. The results of the antioxidant activity of all samples were categorized as very strong antioxidants as stated in the IC value50 &amp;lt; 50 ppm. Optimizing the antioxidant serum formulation used HPMC concentrations of 0.5%, 1% and 2% respectively. The results obtained from testing antioxidant activity obtained IC values50 the best was 0.368 ppm for the ethyl acetate fraction of red pomegranate peel. Optimizing the serum formula used a HPMC concentration of 1%, with an IC value50 amounting to 133,903 ppm.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-07-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/JSTRP/article/view/10664</dc:identifier>
	<dc:identifier>10.15294/jstrp.v5i1.10664</dc:identifier>
	<dc:source xml:lang="en">Journal of Science and Technology Research for Pharmacy; Vol. 5 No. 1 (2025): Journal of Science and Technology Research for Pharmacy; 40-51</dc:source>
	<dc:source>2776-0685</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/JSTRP/article/view/10664/5151</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Science and Technology Research for Pharmacy</dc:rights>
</oai_dc:dc>