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Vanilla Callogenesis with the Addition of Picloram and BAP under Dark and Light Conditions

Life Science · Vol. 14 No. 1 (2025) · hlm. 11–21

DOI
10.15294/unnesjlifesci.v14.i1.22275
ISSN
2528-5009, 2252-6277
Terbit
2025-05-31
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Vanillin is a secondary metabolite in vanilla, with a 1%–3% content. Tissue culture is an effective alternative for producing plant secondary metabolites. This study aimed to analyze the effect of picloram and BAP under light and dark conditions on the callogenesis of vanilla leaf explants. A randomized block design was used with two factors: picloram concentration (0 ppm, 2.5 ppm, 5 ppm, 7.5 ppm) and BAP (0 ppm, 1 ppm, 2 ppm, 3 ppm), as well as lighting conditions (light and dark). Vanilla leaf explants were cultured on MS medium and incubated for three months. The observed parameters included the time for explants to curve, the percentage of curved explants, the time of callus formation, the percentage of callus-forming explants, and callus morphology. The results showed that PGRs type/concentration significantly affected the percentage of curved explants but had no significant effect on other parameters. Lighting and its interaction with PGRs had no significant effect. The best combination of picloram and BAP resulted in 100% curved explants but was less optimal for inducing callus formation in vanilla leaf explants. This study can open scientific insights into the combination of picloram and BAP and the effects of different lighting in vanilla leaf callogenesis.

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  • State University of Semarang
  • Diponegoro University
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Rekan penulisOrganisasiArtikel bersama
Enni Suwarsi RahayuState University of Semarang3
Furzani Pa’eeAfiliasi tidak diketahui2
Lina HerlinaDiponegoro University2
Sholihatur RohmahAfiliasi tidak diketahui1
Talitha WidiatningrumAfiliasi tidak diketahui1
Nur WijawatiAfiliasi tidak diketahui1
Fajar MusafaAfiliasi tidak diketahui1
Khoirul MukhtarAfiliasi tidak diketahui1
Y. Ulung AnggraitoAfiliasi tidak diketahui1
Anisa Dewi Sri PuspitasariAfiliasi tidak diketahui1
Adninta Husnu AmaliaAfiliasi tidak diketahui1
Nurul Huda Am Zen AriefAfiliasi tidak diketahui1
Yustinus Ulung AnggraitoAfiliasi tidak diketahui1
Dinda MelatiAfiliasi tidak diketahui1
Na Dame Maulibulung SinagaAfiliasi tidak diketahui1
Siti AlimahState University of Semarang1
Vira AyuningrumAfiliasi tidak diketahui1
Mualimin -Afiliasi tidak diketahui1
Krispinus Kedati PukanState University of Semarang1
Griyani -Afiliasi tidak diketahui1
Titis PurwantiAfiliasi tidak diketahui1
Supriyanto SupriyantoAfiliasi tidak diketahui1
Ika MundiyakinAfiliasi tidak diketahui1
Nana Kariada Tri MartutiAfiliasi tidak diketahui1
Annisaa NurjannahAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/UnnesJLifeSci/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 5 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Vanilla Callogenesis with the Addition of Picloram and BAP under Dark and Light Conditions</dc:title>
	<dc:creator xml:lang="en">Bahrunnisa, Atiqah</dc:creator>
	<dc:creator xml:lang="en">Noor Aini Habibah</dc:creator>
	<dc:subject xml:lang="en">BAP</dc:subject>
	<dc:subject xml:lang="en">Callus</dc:subject>
	<dc:subject xml:lang="en">Lighting</dc:subject>
	<dc:subject xml:lang="en">Picloram</dc:subject>
	<dc:subject xml:lang="en">Vanilla</dc:subject>
	<dc:description xml:lang="en">Vanillin is a secondary metabolite in vanilla, with a 1%–3% content. Tissue culture is an effective alternative for producing plant secondary metabolites. This study aimed to analyze the effect of picloram and BAP under light and dark conditions on the callogenesis of vanilla leaf explants. A randomized block design was used with two factors: picloram concentration (0 ppm, 2.5 ppm, 5 ppm, 7.5 ppm) and BAP (0 ppm, 1 ppm, 2 ppm, 3 ppm), as well as lighting conditions (light and dark). Vanilla leaf explants were cultured on MS medium and incubated for three months. The observed parameters included the time for explants to curve, the percentage of curved explants, the time of callus formation, the percentage of callus-forming explants, and callus morphology. The results showed that PGRs type/concentration significantly affected the percentage of curved explants but had no significant effect on other parameters. Lighting and its interaction with PGRs had no significant effect. The best combination of picloram and BAP resulted in 100% curved explants but was less optimal for inducing callus formation in vanilla leaf explants. This study can open scientific insights into the combination of picloram and BAP and the effects of different lighting in vanilla leaf callogenesis. </dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/UnnesJLifeSci/article/view/22275</dc:identifier>
	<dc:identifier>10.15294/unnesjlifesci.v14.i1.22275</dc:identifier>
	<dc:source xml:lang="en">Life Science; Vol. 14 No. 1 (2025): May 2025; 11-21</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:source>10.15294/unnesjlifesci.v14.i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/UnnesJLifeSci/article/view/22275/4210</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Life Science</dc:rights>
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