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STUDI AKTIVITAS ANTIBAKTERI SARI DAUN BINAHONG (Anredera cordifolia) TERHADAP PERTUMBUHAN Bacillus cereus dan Salmonella enteritidis

Life Science · Vol. 3 No. 2 (2015)

ISSN
2528-5009, 2252-6277
Published
2015-01-29
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri sari daun binahong (Anredera cordifolia) terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis. Sampel daun binahong diperoleh dari Desa Gentan Kecamatan Bendosari Kabupaten Sukoharjo. Konsentrasi sari daun binahong yang digunakan adalah 25 %, 50 %, 75 %, dan 100 %, dengan antibiotik kotrimoksazol sebagai kontrol positif. Uji aktivitas antibakteri sari daun binahong terhadap bakteri Bacillus cereus dan Salmonella enteritidis dilakukan secara in vitro menggunakan metode difusi kertas cakram melalui pengukuran diameter zona hambat. Data dianalisis one way Anova dengan tingkat kepercayaan 95% dan diuji lanjut LSD. Hasil penelitian menunjukkan bahwa sari daun binahong pada konsentrasi 100% dapat menghambat pertumbuhan Bacillus cereus dan Salmonella enteritidis masing-masing sebesar 9,64 mm dan 6,86 mm. Konsentrasi hambat minimum yaitu pada konsentrasi 25% dengan zona hambat 2,54 mm pada B. cereus dan 2,52 mm pada Salmonella enteritidis. Simpulan dari penelitian ini adalah daun binahong memiliki aktivitas antibakteri terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis, dan aktivitas antibakteri lebih besar ditunjukkan pada bakteri Gram positif yaitu Bacillus cereus. This study aims to determine the antibacterial activity of the binahong leaf extract on the growth of Bacillus cereus and Salmonella enteritidis. The sample is derived from plants grown in Gentan Bendosari Sukoharjo. The concentrations of the binahong leaf extract used are 25 %, 50 %, 75%, and 100%, with the antibiotic cotrimoxazole as a positive control. The test of binahong leaf extract antibacterial activity against bacterial of Bacillus cereus and Salmonella enteritidis is done in vitro using paper disc diffusion method by measuring the diameter of inhibition zone. The data is analyzed using one way Anova with 95% trust level and LSD test further. The results showed that the binahong leaf extract at a concentration of 100 % can inhibit 9,64 mm of Bacillus cereus growth and 6,86 mm of Salmonella enteritidis growth. The minimum inhibition concentration is at a concentration of 25 % with the inhibition zone 2,54 mm for Bacillus cereus and 2,52 mm for Salmonella enteritidis. The conclusions of this study is binahong leaves have antibacterial activity against the growth of Bacillus cereus and Salmonella enteritidis, greater antibacterial activity shown in Gram positive bacteria that Bacillus cereus.

Collaboration network of the authors

36 authors · 77 co-authorship links · 1 across different organisations

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  • State University of Semarang
  • Universitas Indraprasta PGRI
  • Universitas Nahdlatul Ulama Blitar
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Dewi MustikaningtyasAffiliation unknown11
Pramesti DewiAffiliation unknown8
Ibnul MubarokAffiliation unknown4
R. SusantiAffiliation unknown2
Nadya Wulan RamadhaniAffiliation unknown1
Mutiara Bintang RamadaniAffiliation unknown1
Astrid FarmayatiAffiliation unknown1
Lusi Maswahyu AndriyaniAffiliation unknown1
SunyotoAffiliation unknown1
Nana Kariada Tri MartutiState University of Semarang1
Fitria Ayu TirtaningrumAffiliation unknown1
Lidya Ayu NataliaAffiliation unknown1
Ruri WinantiAffiliation unknown1
Anis MaftuhahAffiliation unknown1
Retno Sri IswariState University of Semarang1
Septi JayantiAffiliation unknown1
Anisa Ratna NugrainiAffiliation unknown1
Nihayatul MilahAffiliation unknown1
Saeful AnhariAffiliation unknown1
Dwi SusilaningsihAffiliation unknown1
Aulia Nuanza AlamAffiliation unknown1
Nikmatul HidayahAffiliation unknown1
Sri UtamiUniversitas Indraprasta PGRI1
Yenni Tyas Wulandari K. EAffiliation unknown1
Afra Fauziah L. PutriAffiliation unknown1
Talitha WidiatningrumAffiliation unknown1
Nadya Audina Nurkhafiya SadikinAffiliation unknown1
Lulu Dar Zulfatunna’imAffiliation unknown1
Anisa Rahmawati PutriAffiliation unknown1
Isnaeni Karunia AnnisaAffiliation unknown1
Fitri AndiniyatiAffiliation unknown1
Sugianto SugiantoUniversitas Nahdlatul Ulama Blitar1
Dwi YuliantiState University of Semarang1
Tri Sri Noor AsihAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">STUDI AKTIVITAS ANTIBAKTERI SARI DAUN BINAHONG (Anredera cordifolia) TERHADAP PERTUMBUHAN Bacillus cereus dan Salmonella enteritidis</dc:title>
	<dc:creator>Rahmawati, Fahmi</dc:creator>
	<dc:creator>Bintari, Siti Harnina</dc:creator>
	<dc:description xml:lang="en-US">Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri sari daun binahong (Anredera cordifolia) terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis. Sampel daun binahong diperoleh dari Desa Gentan Kecamatan Bendosari Kabupaten Sukoharjo. Konsentrasi sari daun binahong yang digunakan adalah 25 %, 50 %, 75 %, dan 100 %, dengan antibiotik kotrimoksazol sebagai kontrol positif. Uji aktivitas antibakteri sari daun binahong terhadap bakteri Bacillus cereus dan Salmonella enteritidis dilakukan secara in vitro menggunakan metode difusi kertas cakram melalui pengukuran diameter zona hambat. Data dianalisis one way Anova dengan tingkat kepercayaan 95% dan diuji lanjut LSD. Hasil penelitian menunjukkan bahwa sari daun binahong pada konsentrasi 100% dapat menghambat pertumbuhan Bacillus cereus dan Salmonella enteritidis masing-masing sebesar 9,64 mm dan 6,86 mm. Konsentrasi hambat minimum yaitu pada konsentrasi 25% dengan zona hambat 2,54 mm pada B. cereus dan 2,52 mm pada Salmonella enteritidis. Simpulan dari penelitian ini adalah daun binahong memiliki aktivitas antibakteri terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis, dan aktivitas antibakteri lebih besar ditunjukkan pada bakteri Gram positif yaitu Bacillus cereus. This study aims to determine the antibacterial activity of the binahong leaf extract on the growth of Bacillus cereus and Salmonella enteritidis. The sample is derived from plants grown in Gentan Bendosari Sukoharjo. The concentrations of the binahong leaf extract used are 25 %, 50 %, 75%, and 100%, with the antibiotic cotrimoxazole as a positive control. The test of binahong leaf extract antibacterial activity against bacterial of Bacillus cereus and Salmonella enteritidis is done in vitro using paper disc diffusion method by measuring the diameter of inhibition zone. The data is analyzed using one way Anova with 95% trust level and LSD test further. The results showed that the binahong leaf extract at a concentration of 100 % can inhibit 9,64 mm of Bacillus cereus growth and 6,86 mm of Salmonella enteritidis growth. The minimum inhibition concentration is at a concentration of 25 % with the inhibition zone 2,54 mm for Bacillus cereus and 2,52 mm for Salmonella enteritidis. The conclusions of this study is binahong leaves have antibacterial activity against the growth of Bacillus cereus and Salmonella enteritidis, greater antibacterial activity shown in Gram positive bacteria that Bacillus cereus.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2015-01-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/4566</dc:identifier>
	<dc:source xml:lang="en-US">Life Science; Vol 3 No 2 (2014)</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/4566/4211</dc:relation>
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