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PENGARUH INFUSA DAUN BELUNTAS (Pluchea indica) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Life Science · Vol. 4 No. 1 (2016)

ISSN
2528-5009, 2252-6277
Published
2016-08-30
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Bau badan merupakan salah satu masalah yang mengganggu kehidupan sehari-hari. Kelenjar apokrin mengandung lemak dan protein, yang apabila di uraikan oleh bakteri akan menimbulkan bau yang tidak enak, bau inilah yang di kenal bau badan. Salah satu bakteri pada bau badan adalah bakteri Staphylococcus epidermidis. Bakteri Staphylococcus epidermidis umumnya telah resisten terhadap antibiotik penisilin dan metisilin. Daun beluntas diduga memiliki potensi antibakteri karena mengandung steroid, alkaloid, fenol dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui adanya pengaruh infusa daun beluntas terhadap pertumbuhan bakteri Staphylococcus epidermidis. Yang digunakan dalam penelitian ini adalah penelitian eksperimen yang dilakukan dilaboratorium Fakultas Kedokteran mikrobiologi molekuler Universitas Diponegoro dengan perlakuan konsentrasi ekstrak (20%, 40%, 60%, 80%, 100%) sedangkan konsentrasi suspensi bakteri Staphylococcus epidermidis adalah 1x106 sel/ml. Metode yang digunakan adalah metode dilusi untuk mengamati MIC (Minimum Inhibitory Concentration) dari nilai OD (optical density) dan mengamati MBC (Minimum Bactericidal Concentration). Hasil penelitian menggunakan analisa deskriptif. Hasilnya menunjukkan bahwa infusa daun beluntas dapat menghambat pertumbuhan Staphylococcus epidermidis. Penelitian menunjukkan MIC adalah konsentrasi 20% dengan selisih nilai OD -0.026 sedangkan MBC infusa daun beluntas terhadap bakteri Staphylococcus epidermidis adalah konsentrasi 100% ditandai dengan sudah tidak munculnya koloni bakteri Staphylococcus epidermidis. Kandungan daun beluntas yang berperan sebagai antibakteri adalah tannin, fenol, flavonoid, sterol dan alkaloid. Disimpulkan bahwa daun beluntas memiliki pengaruh antibakteri terhadap Staphylococcus epidermidis secara in vitro. Body odor is one of the problems that interfere with daily life. Apocrine glands contain fat and protein, which when is described by the bacteria will cause an unpleasant smell, the smell is what is known body odor. One of the bacteria in the body odor is the bacteria Staphylococcus epidermidis. Staphylococcus epidermidis bacteria are generally resistant to antibiotics penicillin and methicillin. Beluntas leaves suspected to have antibacterial potency because they contain steroids, alkaloids, phenols and flavonoids. The aim of this study was to investigate the effect of infusion of leaves beluntas on the growth of Staphylococcus epidermidis. Used in this study is an experimental research conducted molecular microbiology laboratory of the Faculty of Medicine, University of Diponegoro by treatment with the extract (20%, 40%, 60%, 80%, 100%), while the concentration of Staphylococcus epidermidis suspension is 1x106 cells / ml. The method used is the dilution method to observe the MIC (Minimum Inhibitory Concentration) of the OD (optical density) and observing the MBC (Minimum Bactericidal Concentration). The results using descriptive analysis. The results show that infusion of leaves beluntas can inhibit the growth of Staphylococcus epidermidis. Research shows MIC is the concentration of 20% with a difference of OD value -0026 while MBC infuse leaves beluntas against Staphylococcus epidermidis was 100% concentration has not marked by the appearance of colonies of Staphylococcus epidermidis. Beluntas leaf content that acts as an antibacterial is tannin, phenols, flavonoids, sterols and alkaloids. Beluntas concluded that the leaves have antibacterial effect against Staphylococcus epidermidis in vitro

Collaboration network of the authors

40 authors · 88 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Diponegoro University
  • Universitas Indraprasta PGRI
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Pramesti DewiAffiliation unknown17
Ibnul MubarokAffiliation unknown5
Talitha WidiatningrumAffiliation unknown4
Andhina Putri HeriyantiDiponegoro University2
R. SusantiAffiliation unknown2
Mutiara Bintang RamadaniAffiliation unknown2
Astrid FarmayatiAffiliation unknown2
Lusi Maswahyu AndriyaniAffiliation unknown2
SunyotoAffiliation unknown2
Lidya Ayu NataliaAffiliation unknown2
Ruri WinantiAffiliation unknown2
Anisa Ratna NugrainiAffiliation unknown2
Nihayatul MilahAffiliation unknown2
Nikmatul HidayahAffiliation unknown2
Supriyadi SupriyadiAffiliation unknown1
Kusmuriyanto KusmuriyantoAffiliation unknown1
Mochamad Nur SholikhinAffiliation unknown1
Nishfa MufatihahAffiliation unknown1
Nadya Wulan RamadhaniAffiliation unknown1
Hasyim AbdurrasyidAffiliation unknown1
Ghani WalandipaAffiliation unknown1
Siti Harnina BintariAffiliation unknown1
Nilam SyifaAffiliation unknown1
Nana Kariada Tri MartutiState University of Semarang1
Fitria Ayu TirtaningrumAffiliation unknown1
Fahmi RahmawatiAffiliation unknown1
Retno Sri IswariState University of Semarang1
Septi JayantiAffiliation unknown1
Saeful AnhariAffiliation unknown1
Dwi SusilaningsihAffiliation unknown1
Aulia Nuanza AlamAffiliation unknown1
Sri UtamiUniversitas Indraprasta PGRI1
Yenni Tyas Wulandari K. EAffiliation unknown1
Ari YuniastutiState University of Semarang1
Intan Ayu ElissaAffiliation unknown1
Afra Fauziah L. PutriAffiliation unknown1
Nadya Audina Nurkhafiya SadikinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc)
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	<dc:title xml:lang="en-US">PENGARUH INFUSA DAUN BELUNTAS (Pluchea indica) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis</dc:title>
	<dc:creator>Maftuhah, Anis</dc:creator>
	<dc:creator>Bintari, Siti Harnina</dc:creator>
	<dc:creator>Mustikaningtyas, Dewi</dc:creator>
	<dc:description xml:lang="en-US">Bau badan merupakan salah satu masalah yang mengganggu kehidupan sehari-hari. Kelenjar apokrin mengandung lemak dan protein, yang apabila di uraikan oleh bakteri akan menimbulkan bau yang tidak enak, bau inilah yang di kenal bau badan. Salah satu bakteri pada bau badan adalah bakteri Staphylococcus epidermidis. Bakteri Staphylococcus epidermidis umumnya telah resisten terhadap antibiotik penisilin dan metisilin. Daun beluntas diduga memiliki potensi antibakteri karena mengandung steroid, alkaloid, fenol dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui adanya pengaruh infusa daun beluntas terhadap pertumbuhan bakteri Staphylococcus epidermidis. Yang digunakan dalam penelitian ini adalah penelitian eksperimen yang dilakukan dilaboratorium Fakultas Kedokteran mikrobiologi molekuler Universitas Diponegoro dengan perlakuan konsentrasi ekstrak (20%, 40%, 60%, 80%, 100%) sedangkan konsentrasi suspensi bakteri Staphylococcus epidermidis adalah 1x106 sel/ml. Metode yang digunakan adalah metode dilusi untuk mengamati MIC (Minimum Inhibitory Concentration) dari nilai OD (optical density) dan mengamati MBC (Minimum Bactericidal Concentration). Hasil penelitian menggunakan analisa deskriptif. Hasilnya menunjukkan bahwa infusa daun beluntas dapat menghambat pertumbuhan Staphylococcus epidermidis. Penelitian menunjukkan MIC adalah konsentrasi 20% dengan selisih nilai OD -0.026 sedangkan MBC infusa daun beluntas terhadap bakteri Staphylococcus epidermidis adalah konsentrasi 100% ditandai dengan sudah tidak munculnya koloni bakteri Staphylococcus epidermidis. Kandungan daun beluntas yang berperan sebagai antibakteri adalah tannin, fenol, flavonoid, sterol dan alkaloid. Disimpulkan bahwa daun beluntas memiliki pengaruh antibakteri terhadap Staphylococcus epidermidis secara in vitro. Body odor is one of the problems that interfere with daily life. Apocrine glands contain fat and protein, which when is described by the bacteria will cause an unpleasant smell, the smell is what is known body odor. One of the bacteria in the body odor is the bacteria Staphylococcus epidermidis. Staphylococcus epidermidis bacteria are generally resistant to antibiotics penicillin and methicillin. Beluntas leaves suspected to have antibacterial potency because they contain steroids, alkaloids, phenols and flavonoids. The aim of this study was to investigate the effect of infusion of leaves beluntas on the growth of Staphylococcus epidermidis. Used in this study is an experimental research conducted molecular microbiology laboratory of the Faculty of Medicine, University of Diponegoro by treatment with the extract (20%, 40%, 60%, 80%, 100%), while the concentration of Staphylococcus epidermidis suspension is 1x106 cells / ml. The method used is the dilution method to observe the MIC (Minimum Inhibitory Concentration) of the OD (optical density) and observing the MBC (Minimum Bactericidal Concentration). The results using descriptive analysis. The results show that infusion of leaves beluntas can inhibit the growth of Staphylococcus epidermidis. Research shows MIC is the concentration of 20% with a difference of OD value -0026 while MBC infuse leaves beluntas against Staphylococcus epidermidis was 100% concentration has not marked by the appearance of colonies of Staphylococcus epidermidis. Beluntas leaf content that acts as an antibacterial is tannin, phenols, flavonoids, sterols and alkaloids. Beluntas concluded that the leaves have antibacterial effect against Staphylococcus epidermidis in vitro</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2016-08-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/12278</dc:identifier>
	<dc:source xml:lang="en-US">Life Science; Vol 4 No 1 (2015)</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/12278/6845</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 Life Science</dc:rights>
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