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Pengaruh Faktor Lingkungan terhadap Pertumbuhan Trichoderma spp. dan Aktivitas Enzim Amilase dan Xilanase

Life Science · Vol. 11 No. 2 (2022) · pp. 108–119

ISSN
2528-5009, 2252-6277
Published
2022-11-30
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Trichoderma erinaceum and Trichoderma koningiopsis have the potential to produce lignocellulolytic enzymes, such as amylase and xylanase. Molds must grow in an ideal environment to support their metabolism, so research is needed to determine the temperature and pH of the mold growth environment and its correlation with the enzymes produced. The research was carried out in several stages, including 1) Testing the effect of temperature and pH on the growth of the mold isolates T. erinaceum and T. koningiopsis, 2) Testing the activity of amylase and xylanase enzymes on the best isolates resulting from variations in temperature and pH. The results showed that the best temperature for growth of T. erinaceum based on the calculation of diameter, density, and mycelium compactness was 25°C, while T. koningiopsis was 25-35°C. The best pH for growth of T. erinaceum based on the calculation of diameter, density, and mycelium compactness was 5-7, while T. koningiopsis was 7. At 25°C and pH 7, T. koningiopsis produced the highest amylase activity, while at 25°C and pH 5, T. erinaceum produced the highest xylanase activity. It is recommended to research the effect of parameters other than temperature and pH on the growth of Trichoderma spp. Trichoderma erinaceum dan Trichoderma koningiopsis berpotensi menghasilkan enzim lignoselulolitik, seperti amilase dan xilanase. Kapang harus tumbuh di lingkungan yang ideal untuk mendukung metabolismenya, sehingga perlu dilakukan penelitian untuk mengetahui suhu dan pH lingkungan pertumbuhan kapang dan korelasinya dengan enzim yang dihasilkan. Penelitian dilakukan dengan beberapa tahap, diantaranya: 1) Uji pengaruh suhu dan pH pada pertumbuhan isolat kapang T. erinaceum dan T. koningiopsis, 2) Uji aktivitas enzim amilase dan xilanase pada isolat terbaik hasil variasi suhu dan pH. Hasil penelitian menunjukkan bahwa suhu terbaik pertumbuhan T. erinaceum berdasarkan penghitungan diameter, kelebatan, dan kekompakan miselium adalah 25°C, sedangkan T. koningiopsis adalah 25-35°C. pH terbaik pertumbuhan T. erinaceum berdasarkan penghitungan diameter, kelebatan, dan kekompakan miselium adalah 5-7, sedangkan T. koningiopsis adalah 7. Pada suhu 25°C dan pH 7, T. koningiopsis menghasilkan aktivitas amilase tertinggi, sedangkan pada suhu 25°C dan pH 5, T. erinaceum menghasilkan aktivitas xilanase tertinggi. Disarankan untuk dilakukan penelitian mengenai pengaruh parameter selain suhu dan pH terhadap pertumbuhan Trichoderma spp. Keywords: Trichoderma spp.; temperature; pH; amylase; xylanase; suhu; pH; amilase; xilanase

Collaboration network of the authors

40 authors · 137 co-authorship links · 1 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Diponegoro University
  • Universitas Sumatera Utara
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Siti Harnina BintariAffiliation unknown20
Ibnul MubarokAffiliation unknown4
Dyah Rini IndriyantiState University of Semarang3
Ghani WalandipaAffiliation unknown3
Priyantini WidiyaningrumAffiliation unknown2
Maria Ayu PuspitaAffiliation unknown2
Nunung Eni ElawatiAffiliation unknown2
Andhina Putri HeriyantiDiponegoro University2
Edi KurniawanUniversitas Sumatera Utara2
Sucihatiningsih Dian Wisika PrajantiState University of Semarang2
Niken SubektiState University of Semarang2
Ning SetiatiAffiliation unknown2
Ely RudyatmiAffiliation unknown2
Hasyim AbdurrasyidAffiliation unknown2
Mutiara Bintang RamadaniAffiliation unknown2
Astrid FarmayatiAffiliation unknown2
Lusi Maswahyu AndriyaniAffiliation unknown2
SunyotoAffiliation unknown2
Ernallah YatiAffiliation unknown1
Aura Tirsa MelindaAffiliation unknown1
Rahimma Artantia AnandaAffiliation unknown1
Orchida Puspa JelitaAffiliation unknown1
Delia Alifia FarrezaAffiliation unknown1
Supriyadi SupriyadiAffiliation unknown1
Kusmuriyanto KusmuriyantoAffiliation unknown1
Mochamad Nur SholikhinAffiliation unknown1
Nishfa MufatihahAffiliation unknown1
Sriyadi SriyadiAffiliation unknown1
Rusyida AuliyaAffiliation unknown1
Naeli Tamin QonitaAffiliation unknown1
Previanda ArditamaAffiliation unknown1
Fira Putri Nur AwwaliaAffiliation unknown1
Nana Kariada Tri MartutiAffiliation unknown1
Elfrida Theresia SiburianAffiliation unknown1
Nur Rahayu UtamiAffiliation unknown1
Stella FlorensiaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Pengaruh Faktor Lingkungan terhadap Pertumbuhan Trichoderma spp. dan Aktivitas Enzim Amilase dan Xilanase</dc:title>
	<dc:creator>Wahidah, Tristiana Hidayatul</dc:creator>
	<dc:creator>Mustikaningtyas, Dewi</dc:creator>
	<dc:creator>Widiatningrum, Talitha</dc:creator>
	<dc:creator>Dewi, Pramesti</dc:creator>
	<dc:description xml:lang="en-US">Trichoderma erinaceum and Trichoderma koningiopsis have the potential to produce lignocellulolytic enzymes, such as amylase and xylanase. Molds must grow in an ideal environment to support their metabolism, so research is needed to determine the temperature and pH of the mold growth environment and its correlation with the enzymes produced. The research was carried out in several stages, including 1) Testing the effect of temperature and pH on the growth of the mold isolates T. erinaceum and T. koningiopsis, 2) Testing the activity of amylase and xylanase enzymes on the best isolates resulting from variations in temperature and pH. The results showed that the best temperature for growth of T. erinaceum based on the calculation of diameter, density, and mycelium compactness was 25°C, while T. koningiopsis was 25-35°C. The best pH for growth of T. erinaceum based on the calculation of diameter, density, and mycelium compactness was 5-7, while T. koningiopsis was 7. At 25°C and pH 7, T. koningiopsis produced the highest amylase activity, while at 25°C and pH 5, T. erinaceum produced the highest xylanase activity. It is recommended to research the effect of parameters other than temperature and pH on the growth of Trichoderma spp.
&amp;nbsp;
Trichoderma erinaceum dan Trichoderma koningiopsis berpotensi menghasilkan enzim lignoselulolitik, seperti amilase dan xilanase. Kapang harus tumbuh di lingkungan yang ideal untuk mendukung metabolismenya, sehingga perlu dilakukan penelitian untuk mengetahui suhu dan pH lingkungan pertumbuhan kapang dan korelasinya dengan enzim yang dihasilkan. Penelitian dilakukan dengan beberapa tahap, diantaranya: 1) Uji pengaruh suhu dan pH pada pertumbuhan isolat kapang T. erinaceum dan T. koningiopsis, 2) Uji aktivitas enzim amilase dan xilanase pada isolat terbaik hasil variasi suhu dan pH. Hasil penelitian menunjukkan bahwa suhu terbaik pertumbuhan T. erinaceum berdasarkan penghitungan diameter, kelebatan, dan kekompakan miselium adalah 25°C, sedangkan T. koningiopsis adalah 25-35°C. pH terbaik pertumbuhan T. erinaceum berdasarkan penghitungan diameter, kelebatan, dan kekompakan miselium adalah 5-7, sedangkan T. koningiopsis adalah 7. Pada suhu 25°C dan pH 7, T. koningiopsis menghasilkan aktivitas amilase tertinggi, sedangkan pada suhu 25°C dan pH 5, T. erinaceum menghasilkan aktivitas xilanase tertinggi. Disarankan untuk dilakukan penelitian mengenai pengaruh parameter selain suhu dan pH terhadap pertumbuhan Trichoderma spp.
Keywords:&amp;nbsp;Trichoderma spp.; temperature; pH; amylase; xylanase ; suhu; pH; amilase; xilanase</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2022-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/64379</dc:identifier>
	<dc:source xml:lang="en-US">Life Science; Vol 11 No 2 (2022): November 2022; 108-119</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:source>10.15294/lifesci.v11i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/64379/23344</dc:relation>
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