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Characterization of Chitinolitic Bacteria from Hermatia illucent Larvae Waste: Antifungal Activity, Hydrolytic Enzyme, and Phosphate-Potassium Solubilization

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 1 (2024) · hlm. 181–190

DOI
10.15294/biosaintifika.v15i1.311
ISSN
2338-7610, 2085-191X
Terbit
2024-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Hermetia illucens larvae, also known as the black soldier fly larvae, are best known as the decomposers of organic matter. There are many potential microbes found in the feces of BSF larvae. This research aimed to isolate the chitinolytic bacteria from chicken manure maggot waste, identify the antifugal activity, hydrolytic enzyme activity, phosphate and potassium solubilization, and bacterial species through 16S rRNA gene analysis. The initial screening focused on bacteria with chitinolytic ability. Antifungal activity tests were performed against phytopathogenic fungi, Colletotrichum sp. Isolate MKP02 showed the highest activity in inhibiting the growth of Colletotrichum sp. up to 100% and produced protease and cellulase enzymes, along with the ability to solubilize potassium. Furthermore, the potential isolate MKP04, the isolate shows the highest cellulolytic activity with a percentage of 300%. It can inhibit Colletotrichum sp. fungi, as well as having lipase enzyme content, and being able to dissolve potassium. The results of 16S rRNA gene amplification on the two isolates showed that both isolates were close to bacteria of the genus Lysinibacillus and Brevibacterium. This research is expected to provide valuable information about the bacterial content, levels of hydrolytic enzymes, and the ability to solubilize phosphate and potassium in BSF.

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22 penulis · 81 tautan kepenulisan bersama · 32 lintas organisasi

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  • National Research and Innovation Agency
  • Montana State University
  • Jenderal Soedirman University
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Rekan penulisOrganisasiArtikel bersama
Harlita HarlitaSebelas Maret University2
Meti IndrowatiSebelas Maret University2
Slamet SantosaSebelas Maret University2
Muhammad Ilham FaturrahimSebelas Maret University1
Maria Indah PurnamasariMontana State University1
Adha NisfatulsanahSebelas Maret University1
Aris Tri WahyudiIPB University1
Anja MeryandiniIPB University1
Abdjad Asih NawangsihIPB University1
Yulin LestariIPB University1
Dewi Puspita SariSebelas Maret University1
Sri Martina WiraswatiJenderal Soedirman University1
Vera PermatasariNational Research and Innovation Agency1
Muhamad Eka PrastyaAfiliasi tidak diketahui1
Indri YatiNational Research and Innovation Agency1
Gian PrimahanaNational Research and Innovation Agency1
Anggie Meilinda ZienithaSebelas Maret University1
Meisi AnggrainiSebelas Maret University1
Lisa RositaSebelas Maret University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Characterization of Chitinolitic Bacteria from Hermatia illucent Larvae Waste: Antifungal Activity, Hydrolytic Enzyme, and Phosphate-Potassium Solubilization</dc:title>
	<dc:creator xml:lang="en">Prameselly, Qorisha Lutfia </dc:creator>
	<dc:creator xml:lang="en">Sugiharto, Bowo</dc:creator>
	<dc:creator xml:lang="en">Fatmawati, Umi</dc:creator>
	<dc:subject xml:lang="en">antifungal</dc:subject>
	<dc:subject xml:lang="en">decomposer</dc:subject>
	<dc:subject xml:lang="en">maggot</dc:subject>
	<dc:description xml:lang="en">Hermetia illucens larvae, also known as the black soldier fly larvae, are best known as the decomposers of organic matter. There are many potential microbes found in the feces of BSF larvae. This research aimed to isolate the chitinolytic bacteria from chicken manure maggot waste, identify the antifugal activity, hydrolytic enzyme activity, phosphate and potassium solubilization, and bacterial species through 16S rRNA gene analysis. The initial screening focused on bacteria with chitinolytic ability. Antifungal activity tests were performed against phytopathogenic fungi, Colletotrichum sp. Isolate MKP02 showed the highest activity in inhibiting the growth of Colletotrichum sp. up to 100% and produced protease and cellulase enzymes, along with the ability to solubilize potassium. Furthermore, the potential isolate MKP04, the isolate shows the highest cellulolytic activity with a percentage of 300%. It can inhibit Colletotrichum sp. fungi, as well as having lipase enzyme content, and being able to dissolve potassium. The results of 16S rRNA gene amplification on the two isolates showed that both isolates were close to bacteria of the genus Lysinibacillus and Brevibacterium. This research is expected to provide valuable information about the bacterial content, levels of hydrolytic enzymes, and the ability to solubilize phosphate and potassium in BSF.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/311</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v15i1.311</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 1 (2024): April 2024; 181-190</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/311/191</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>