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The Alteration Level of VEGF of Colon Cancer Cell After Induction with Mandarin Orange (Citrus Reticulata) Peel Ethanol Extract

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 2 (2024) · hlm. 198–205

DOI
10.15294/biosaintifika.v16i2.3401
ISSN
2338-7610, 2085-191X
Terbit
2024-08-15
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

The natural ingredient-based therapy extracted from various plants is assumed more tolerable to cancer patients’ bodies than synthetic drugs. To tell successful novel cancer therapy, an indicator is needed to confirm the result. VEGF is a pro-angiogenic factor that leads the cancer cells’ invasion to another organ and is associated with high mortality in patients. Hence, the VEGF level is widely used as one of the successful cancer therapy indicators. Purposed of research elucidated the effect of Mandarin orange peel ethanol extract (EEKJM) to improve the reduction of VEGF levels in colon cancer in vitro. This study was an experimental posttest and control group design to analyze different level of VEGF on cancer cells at end of the study. Research began with the preparation of EEKJM followed by a cytotoxic extract test using the MTT assay method. Furthermore, the measurement of VEGF levels by the VEGF ELISA kit protocol. One Way ANOVA was performed to analyze data.Results showed the cytotoxic test of the EEKJM and doxorubicin against WiDr cells was 240 g/ml and 1.59 µg/ml. VEGF levels showed decreasing after being induced with EEKJM and doxorubicin with doses ½ IC50 and ¼ IC50. Conclusion: EEKJM was able to reduce VEGF levels and viability of WiDr colon cancer cells. The advantages are as much as initial evidence of the ability of the orange to treat cancer even though further animal studies or evidence of a pathway of decrease of the VEGF or other signals are required.

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  • Muhammadiyah University of Yogyakarta
  • Universitas Gadjah Mada
  • Universitas Respati Yogyakarta
  • Kobe University Hospital
  • Universitas Muhammadiyah Pontianak
  • Universitas Islam Negeri Sultan Maulana Hasanuddin Banten
  • Nara Institute of Science and Technology
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Rekan penulisOrganisasiArtikel bersama
Hanum Mukti RahayuUniversitas Muhammadiyah Pontianak2
Langkah SembiringUniversitas Gadjah Mada2
Yekti Asih PurwestriUniversitas Gadjah Mada2
Anis Uswatun KhasanahUniversitas Islam Negeri Sultan Maulana Hasanuddin Banten2
Masashi KawaichiNara Institute of Science and Technology1
Rum WijayantiMuhammadiyah University of Yogyakarta1
Ikhlas Muhammad JenieMuhammadiyah University of Yogyakarta1
Aulia PrimasariMuhammadiyah University of Yogyakarta1
Siti FadlilahUniversitas Respati Yogyakarta1
Santi DamayantiUniversitas Respati Yogyakarta1
Ariyanto NugrohoUniversitas Respati Yogyakarta1
Sugiyarto SugiyartoPoliteknik Kesehatan Kemenkes Surakarta1
Herry SusantoSultan Agung Islamic University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">The Alteration Level of VEGF of Colon Cancer Cell After Induction with Mandarin Orange (Citrus Reticulata) Peel Ethanol Extract</dc:title>
	<dc:creator xml:lang="en">Astuti, Yoni</dc:creator>
	<dc:creator xml:lang="en">Zuliana Utami, Anisa </dc:creator>
	<dc:creator xml:lang="en">Suharto, Agus </dc:creator>
	<dc:creator xml:lang="en">Yano, Yoshihiko </dc:creator>
	<dc:creator xml:lang="en">Aristyaning Putri, Wahyu </dc:creator>
	<dc:subject xml:lang="en">Cell culture</dc:subject>
	<dc:subject xml:lang="en">Citrus reticulata</dc:subject>
	<dc:subject xml:lang="en">colon cancer cell</dc:subject>
	<dc:subject xml:lang="en">cytotoxic test</dc:subject>
	<dc:subject xml:lang="en">VEGF</dc:subject>
	<dc:description xml:lang="en">The natural ingredient-based therapy extracted from various plants is assumed more tolerable to cancer patients’ bodies than synthetic drugs. To tell successful novel cancer therapy, an indicator is needed to confirm the result. VEGF is a pro-angiogenic factor that leads the cancer cells’ invasion to another organ and is associated with high mortality in patients. Hence, the VEGF level is widely used as one of the successful cancer therapy indicators. Purposed of research elucidated the effect of Mandarin orange peel ethanol extract (EEKJM) to improve the reduction of VEGF levels in colon cancer in vitro. This study was an experimental posttest and control group design to analyze different level of VEGF on cancer cells at end of the study.  Research began with the preparation of EEKJM followed by a cytotoxic extract test using the MTT assay method. Furthermore, the measurement of VEGF levels by the VEGF ELISA kit protocol. One Way ANOVA was performed to analyze data.Results showed the cytotoxic test of the EEKJM and doxorubicin against WiDr cells was 240 g/ml and 1.59 µg/ml. VEGF levels showed decreasing after being induced with EEKJM and doxorubicin with doses ½ IC50 and ¼ IC50. Conclusion: EEKJM was able to reduce VEGF levels and viability of WiDr colon cancer cells. The advantages are as much as initial evidence of the ability of the orange to treat cancer even though further animal studies or evidence of a pathway of decrease of the VEGF or other signals are required.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-08-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/3401</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v16i2.3401</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 2 (2024): August 2024; 198-205</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/3401/1163</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>