Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural

Soelistijono, R.; Daryanti, Daryanti; Rakhmawati, Dian; Rianto, Prisma Aditya; Utomo, Herry

doi:10.15294/biosaintifika.v15i1.3498

Jurnal

Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 1 (2024) · hlm. 1–9

DOI: 10.15294/biosaintifika.v15i1.3498
ISSN: 2338-7610, 2085-191X
Terbit: 2024-04-20
Penerbit: Universitas Negeri Semarang
Bahasa: eng

Abstrak

Vanda tricolor is currently a protected species because is the occurrence of orchid late blight caused by the Fusarium sp. pathogenic fungus. The objective of this study was to investigate the effectiveness of Rhizoctonia mycorrhizae in inducing resistance against Fusarium sp., the causative agent of late blight, in V. tricolor. The research employed CRBD with three treatments and six replications. Rhizoctonia mycorrhizae were isolated and identified following the procedure outlined by Bayman. Pre-inoculation of Rhizoctonia mycorrhizae was conducted on PDA media, and mycorrhizae were subsequently inoculated onto the roots of V. tricolor seedlings derived from tissue culture. The presence of peloton structures on the roots was determined using the Nakano methods. The Saravanan method was employed to measure peroxidase activity. The results demonstrated that V. tricolor induced by Rhizoctonia mycorrhizae exhibited resistance against Fusarium sp. and displayed the formation of peroxidase enzymes, peloton structures, and lignification. In contrast, the non-induced orchids suffered significant damage to the epidermal tissue of the leaves, did not exhibit an increase in peroxidase enzymes, and failed to form peloton or lignified structures. The novelty of this research is the use of Rhizoctonia mycorrhizae to control orchid late blight diseases at Mount Merapi.

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14 penulis · 33 tautan kepenulisan bersama · 11 lintas organisasi

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Universitas Tunas Pembangunan
Sebelas Maret University
Louisiana State University Agricultural Center
Lampung University
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Rekan penulis	Organisasi	Artikel bersama
Daryanti Daryanti	Universitas Tunas Pembangunan	2
Raden Soelistijono	Universitas Tunas Pembangunan	2
Albertus Sentot Sudarwanto	Sebelas Maret University	1
Rosita Candrakirana	Sebelas Maret University	1
Febryani Sabatira	Lampung University	1
Haryuni Haryuni	Universitas Tunas Pembangunan	1
Irvansyah Cahya Perwita	Universitas Tunas Pembangunan	1
Siti Mardhikasari	Sebelas Maret University	1
Tyara Yunika Sari	Universitas Tunas Pembangunan	1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en">Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural</dc:title>
	<dc:creator xml:lang="en">Soelistijono, R.</dc:creator>
	<dc:creator xml:lang="en">Daryanti, Daryanti</dc:creator>
	<dc:creator xml:lang="en">Rakhmawati, Dian</dc:creator>
	<dc:creator xml:lang="en">Rianto, Prisma Aditya</dc:creator>
	<dc:creator xml:lang="en">Utomo, Herry</dc:creator>
	<dc:subject xml:lang="en">Fusarium sp.; induced resistance; orchid late blight; Rhizoctonia mycorrhiza</dc:subject>
	<dc:description xml:lang="en">Vanda tricolor is currently a protected species because is the occurrence of orchid late blight caused by the Fusarium sp. pathogenic fungus. The objective of this study was to investigate the effectiveness of Rhizoctonia mycorrhizae in inducing resistance against Fusarium sp., the causative agent of late blight, in V. tricolor. The research employed CRBD with three treatments and six replications. Rhizoctonia mycorrhizae were isolated and identified following the procedure outlined by Bayman. Pre-inoculation of Rhizoctonia mycorrhizae was conducted on PDA media, and mycorrhizae were subsequently inoculated onto the roots of V. tricolor seedlings derived from tissue culture. The presence of peloton structures on the roots was determined using the Nakano methods. The Saravanan method was employed to measure peroxidase activity. The results demonstrated that V. tricolor induced by Rhizoctonia mycorrhizae exhibited resistance against Fusarium sp. and displayed the formation of peroxidase enzymes, peloton structures, and lignification. In contrast, the non-induced orchids suffered significant damage to the epidermal tissue of the leaves, did not exhibit an increase in peroxidase enzymes, and failed to form peloton or lignified structures. The novelty of this research is the use of Rhizoctonia mycorrhizae to control orchid late blight diseases at Mount Merapi.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/3498</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v15i1.3498</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 1 (2024): April 2024; 1-9</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/3498/171</dc:relation>
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