Jurnal
Therapeutic Potential of Secretome Hypoxia Mesenchymal Stem Cells: Downregulation of TNF-α and HIF-2α in Metabolic Syndrome-Induced Inflammation in Wistar Rats
Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 2 (2024) · pp. 342–352
- ISSN
- 2338-7610, 2085-191X
- Published
- 2024-08-15
- Publisher
- Universitas Negeri Semarang
- Language
- eng
Abstract
Background: Metabolic syndrome (MetS) has become a global health challenge with several associated issues, such as obesity, insulin resistance, dyslipidemia, and hypertension. Important proteins such as Tumor Necrosis Factor-alpha (TNF-α) and Hypoxia Inducible Factor-2 alpha (HIF-2α) regulate the inflammatory process by inducing the expression of pro-inflammatory proteins. Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) are immunomodulatory, anti-inflammatory, and angiogenic stimulators, which can regulate various inflammatory diseases, including MetS. Objective: This study aims to determine the effect of administering SH-MSCSs on the expression of the TNF-α and Hypoxia Inducible Factor (HIF)-2α genes in the male Wistar rat model with Metabolic Syndrome. Method: This research is an experimental study with a Post-test Only Control Group Design, using a total of 24 male Wistar rats divided into four groups: T1 (Healthy control), T2 (MetS + NaCl), T3 (MetS + administration of SH-MSCs dose 150 uL), and T4 (MetS + administration of SH-MSCs dose 300 uL). SH-MSCSs were administered intraperitoneally four times over 14 days. Adipose tissue TNF-α and HIF-2α gene expression were measured on day 15 using qRT-PCR. Results: TNF-α and HIF-2α gene expression was significantly lower in T3 and T4, compared with the MetS control group (T2). Conclusion: Administration of SH-MSCs was able to reduce the expression of the Tumor Necrosis Factor (TNF-α) and Hypoxia Inducible Factor (HIF)-2α genes in fatty tissue in the male Wistar rat model with Metabolic Syndrome.
Keywords
Collaboration network of the authors
21 authors · 72 co-authorship links
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- University of Brawijaya
- Affiliation unknown
- Different organisations
- Same organisation
- Affiliation unknown
- Thicker line = more joint articles · bigger circle = more articles
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| Co-author | Organisation | Joint articles |
|---|---|---|
| Carolina Kiwik Rahardja | Affiliation unknown | 2 |
| Aditya Marianti | Affiliation unknown | 1 |
| Sri Mursiti | Affiliation unknown | 1 |
| Faya Nuralda Sitompul | Affiliation unknown | 1 |
| Shafira Septiana Futri | Affiliation unknown | 1 |
| Legendra Gantar Negara | Affiliation unknown | 1 |
| Intan Kharyna Sholehah | Affiliation unknown | 1 |
| Dian Sri Asmorowati | Affiliation unknown | 1 |
| Putri Dyah Astari | Affiliation unknown | 1 |
| Cleveria Jessika | Affiliation unknown | 1 |
| Titiek Sumarawati | Affiliation unknown | 1 |
| Eko Setiawan | University of Brawijaya | 1 |
| Yoshi Puspitasari | Affiliation unknown | 1 |
Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.