Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves

Hardjo, Popy Hartatie; Askitosari, Theresia Desy; Wijaya, Agnes Natalia; Edbert, Joshua; Artadana, Ida Bagus Made; Wittayathanarattana, Takon

doi:10.15294/biosaintifika.v16i3.12057

Jurnal

Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 3 (2024) · hlm. 427–436

DOI: 10.15294/biosaintifika.v16i3.12057
ISSN: 2338-7610, 2085-191X
Terbit: 2024-12-24
Penerbit: Universitas Negeri Semarang
Bahasa: eng

Abstrak

Acehnese Patchouli var. Sidikalang can produce patchouli oil, one of the country's sources of foreign exchange, and it is advantageous in many industries. However, patchouli alcohol (PA) content in patchouli oil is still low. The higher PA content determines the better quality of patchouli oil. So, delignification with Trichoderma viride was carried out to increase PA and yield of patchouli oil. The duration of delignification patchouli leaves is one factor affecting the increase in PA and yield. This study aimed to determine the effect of delignification duration on PA content and yield. The research method was completely randomized with 8 treatments (a combination of 2 treatments: with and without delignification, and 4 delignification durations: 0, 3, 6, and 9 days). The inoculum used for delignification was an inoculum ball, and then patchouli leaf samples were extracted using microwave-assisted extraction (MAE). Gas chromatography was carried out for PA analysis. Delignification of patchouli leaves, with a delignification time of 9 days, showed high PA and yield, which were (0.3129 ± 0.1557)% and (1.4543 ± 0.7717)%, respectively. The success of delignification at 9 days was also shown by increased phenolic content. Trichoderma viride is often utilized in delignification process, but the optimal duration of its delignification of Aceh patchouli leaves that can help improve patchouli oil quality is not yet known. This research is hoped to help related industries improve the secondary metabolites of plants, which has many benefits for the community. Implication/benefit for science development/society.

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University of Surabaya
Universitas Jember
Mahidol University

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Rekan penulis	Organisasi	Artikel bersama
Parawita Dewanti	Universitas Jember	1
Mohammad Candra Prayoga	Universitas Jember	1
Sigit Soeparjono	Universitas Jember	1
Tri Handoyo	Universitas Jember	1
Didik Pudji Restanto	Universitas Jember	1
Kanyaratt Supaibulwatana	Mahidol University	1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.

Metadata asli (oai_dc)

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	<dc:title xml:lang="en">Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves</dc:title>
	<dc:creator xml:lang="en">Hardjo, Popy Hartatie</dc:creator>
	<dc:creator xml:lang="en">Askitosari, Theresia Desy </dc:creator>
	<dc:creator xml:lang="en">Wijaya, Agnes Natalia </dc:creator>
	<dc:creator xml:lang="en">Edbert, Joshua </dc:creator>
	<dc:creator xml:lang="en">Artadana, Ida Bagus Made </dc:creator>
	<dc:creator xml:lang="en">Wittayathanarattana, Takon </dc:creator>
	<dc:subject xml:lang="en">Duration of delignification; Delignification; Phenolic; Yield</dc:subject>
	<dc:description xml:lang="en">Acehnese Patchouli var. Sidikalang can produce patchouli oil, one of the country's sources of foreign exchange, and it is advantageous in many industries. However, patchouli alcohol (PA) content in patchouli oil is still low. The higher PA content determines the better quality of patchouli oil. So, delignification with Trichoderma viride was carried out to increase PA and yield of patchouli oil. The duration of delignification patchouli leaves is one factor affecting the increase in PA and yield. This study aimed to determine the effect of delignification duration on PA content and yield. The research method was completely randomized with 8 treatments (a combination of 2 treatments: with and without delignification, and 4 delignification durations: 0, 3, 6, and 9 days). The inoculum used for delignification was an inoculum ball, and then patchouli leaf samples were extracted using microwave-assisted extraction (MAE). Gas chromatography was carried out for PA analysis. Delignification of patchouli leaves, with a delignification time of 9 days, showed high PA and yield, which were (0.3129 ± 0.1557)% and (1.4543 ± 0.7717)%, respectively. The success of delignification at  9 days was also shown by increased phenolic content. Trichoderma viride is often utilized in delignification process, but the optimal duration of its delignification of Aceh patchouli leaves that can help improve patchouli oil quality is not yet known. This research is hoped to help related industries improve the secondary metabolites of plants, which has many benefits for the community. Implication/benefit for science development/society.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-12-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/12057</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v16i3.12057</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 3 (2024): December 2024; 427-436</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/12057/2228</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>