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Toxicity And Antioxidant Activities of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea Linn.)

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 3 (2024) · hlm. 415–426

DOI
10.15294/biosaintifika.v16i3.13360
ISSN
2338-7610, 2085-191X
Terbit
2024-12-24
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Endophytic bacteria are bacteria that live in healthy plant tissues without causing damage. Several studies have reported that endophytic bacteria can produce active compounds similar to those secreted by their host and which potentially have medicinal value. Butterfly pea (Clitoria ternatea L.) was noted to be able to produce antioxidants and have toxicity potential from its compounds. Therefore, endophytic bacteria from butterfly pea have great potential to have antioxidant activity along with evaluating the toxicity level of the selected bacteria. This study aimed to determine the number of isolates, to characterize, and test the toxicity and antioxidant activities of endophytic bacteria from butterfly pea. Toxicity level was tested using the Brine Shrimp Lethality Test (BSLT) method while levels of antioxidants were tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. A total of fifteen endophytic bacteria were obtained and successfully purified. Based on the morphological observations, Gram staining, and biochemical test results, isolate EBT13 was determined to belong to the genus Bacillus. Isolate EBT13 was categorized as highly toxic, with the highest toxicity value with other bacterial isolates with an LC50 of 84 ppm and antioxidant activity with an IC50 value of 44.32 ppm. Based on the phylogenetic tree of 16S rRNA gene analysis, EBT13 belongs to the genus Bacillus, it forms a sister group with Bacillus pumilus with a bootstrap value of 100%. This study advances our knowledge of plant-microbe interactions by identifying a highly toxic, antioxidant-producing strain of bacteria of butterfly pea. The results have significance for the development of cytotoxic chemicals and natural antioxidants, which could advance biotechnological research and boost therapeutic purpose.

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  • Universitas Halu Oleo
  • Universitas Tamansiswa Padang
  • Hasanuddin University
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Rekan penulisOrganisasiArtikel bersama
Cut YulvizarAfiliasi tidak diketahui2
Sri RahayuHasanuddin University2
Alia RizkiAfiliasi tidak diketahui1
Wira DharmaAfiliasi tidak diketahui1
Yekki YasminAfiliasi tidak diketahui1
Nada AriqahAfiliasi tidak diketahui1
Essy HarnellyAfiliasi tidak diketahui1
Amirunnas AmirunnasAfiliasi tidak diketahui1
Anita RauzanaAfiliasi tidak diketahui1
Rahmad ZakariaAfiliasi tidak diketahui1
Zairin ThomyAfiliasi tidak diketahui1
Muslim MuslimUniversitas Halu Oleo1
Sugiarti SugiartiAfiliasi tidak diketahui1
Misrahanum MisrahanumAfiliasi tidak diketahui1
Ria CerianaAfiliasi tidak diketahui1
Widya SariAfiliasi tidak diketahui1
Dilla SriwahyuniAfiliasi tidak diketahui1
Siti FaizahAfiliasi tidak diketahui1
Rafika Nila KandiAfiliasi tidak diketahui1
Alfinatul RahmiAfiliasi tidak diketahui1
Lisa MaulidinaAfiliasi tidak diketahui1
Syahrial SyahrialUniversitas Tamansiswa Padang1
Wardinal WardinalAfiliasi tidak diketahui1
Safika SafikaAfiliasi tidak diketahui1
Saida RasnoviAfiliasi tidak diketahui1
Ayu RahmadaniAfiliasi tidak diketahui1
Muhammad Rizqi AuliaAfiliasi tidak diketahui1
Eva AgustrianaAfiliasi tidak diketahui1
yuniati yuniatiAfiliasi tidak diketahui1
Rosa MardianaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Toxicity And Antioxidant Activities of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea Linn.)</dc:title>
	<dc:creator xml:lang="en">Zahrina, Yuni </dc:creator>
	<dc:creator xml:lang="en">Fitri, Lenni</dc:creator>
	<dc:creator xml:lang="en">Ismail, Yulia Sari </dc:creator>
	<dc:creator xml:lang="en">Dita, Sasmiati Farach </dc:creator>
	<dc:subject xml:lang="en">antioxidant; endophytic bacteria; Bacillus pumilus; Clitoria ternatea L.; toxicity</dc:subject>
	<dc:description xml:lang="en">Endophytic bacteria are bacteria that live in healthy plant tissues without causing damage. Several studies have reported that endophytic bacteria can produce active compounds similar to those secreted by their host and which potentially have medicinal value. Butterfly pea (Clitoria ternatea L.) was noted to be able to produce antioxidants and have toxicity potential from its compounds. Therefore, endophytic bacteria from butterfly pea have great potential to have antioxidant activity along with evaluating the toxicity level of the selected bacteria. This study aimed to determine the number of isolates, to characterize, and test the toxicity and antioxidant activities of endophytic bacteria from butterfly pea. Toxicity level was tested using the Brine Shrimp Lethality Test (BSLT) method while levels of antioxidants were tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. A total of fifteen endophytic bacteria were obtained and successfully purified. Based on the morphological observations, Gram staining, and biochemical test results, isolate EBT13 was determined to belong to the genus Bacillus. Isolate EBT13 was categorized as highly toxic, with the highest toxicity value with other bacterial isolates with an LC50 of 84 ppm and antioxidant activity with an IC50 value of 44.32 ppm. Based on the phylogenetic tree of 16S rRNA gene analysis, EBT13 belongs to the genus Bacillus, it forms a sister group with Bacillus pumilus with a bootstrap value of 100%. This study advances our knowledge of plant-microbe interactions by identifying a highly toxic, antioxidant-producing strain of bacteria of butterfly pea. The results have significance for the development of cytotoxic chemicals and natural antioxidants, which could advance biotechnological research and boost therapeutic purpose.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-12-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/13360</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v16i3.13360</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 3 (2024): December 2024; 415-426</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/13360/2227</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>