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Effect of Light Intensity on Growth and Primary Metabolites Content of Navicula sp.

Biosaintifika: Journal of Biology & Biology Education · Vol. 16 No. 3 (2024) · hlm. 498–507

DOI
10.15294/biosaintifika.v16i3.13899
ISSN
2338-7610, 2085-191X
Terbit
2024-12-24
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Global energy demand is steadily rising, necessitating the exploration of sustainable alternatives. Microalgae, such as Navicula sp., offer a promising solution. This study investigated the impact of light intensity on the growth, and biochemical composition (carbohydrates, lipids, proteins, and carotenoids) of Navicula sp. Cultures were grown in f/2 medium under four light intensity treatments: 2100 lux (control), 3500 lux, 4500 lux, and 5500 lux. Cell growth was measured through optical density analysis. Carbohydrate, lipid, protein, and carotenoid assays were performed using phenol-sulfuric acid, Bligh and Dyer, Bradford, and spectrophotometric methods. Maximum growth was observed on the eighth day of cultivation at 4500 lux light intensity. The best specific growth rate was achieved at 5500 lux. The highest biomass, carbohydrate, lipid, and protein productivity were attained at 5500 lux (24.65 mg/mL, 0.374 mg/mL/day, 0.399±0.204 mg/mL/day, and 0.025±0.018 mg/mL/day). Conversely, the highest carotenoid productivity was recorded at 2100 lux, 0.278±0.085 mg/mL/day. By those means, the increase in light intensity was positively correlated with microalgal cell growth, biomass, carbohydrate content, and carbohydrate, lipid, and protein productivity. However, a negative correlation was found with carotenoid pigment productivity. From this research, it is evident that a light intensity of 5500 lux offers a feasible option for the industrial-scale cultivation of Navicula sp. microalgae for biomass, carbohydrate, and protein production, while 2100 lux is more suitable for carotenoid production. This research compares the findings to previous studies, emphasizing the unique way Navicula sp. responds to different light levels.

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  • Universitas Gadjah Mada
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Tia ErfiantiAfiliasi tidak diketahui14
Arief BudimanAfiliasi tidak diketahui10
Ria AmeliaAfiliasi tidak diketahui7
Brilian Ryan SadewoAfiliasi tidak diketahui6
Nugroho DewayantoAfiliasi tidak diketahui5
Khusnul Qonita MaghfirohAfiliasi tidak diketahui4
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Fatkhurokhman FauziAfiliasi tidak diketahui1
Muhammad Arif Nurman AryaAfiliasi tidak diketahui1
Insyiraah Oxaichiko ArissintaAfiliasi tidak diketahui1
Indah Dwi SulistiyawatiAfiliasi tidak diketahui1
Falikhatun FalikhatunAfiliasi tidak diketahui1
Sitoresmi PrabaningtyasAfiliasi tidak diketahui1
Dwi ListyoriniAfiliasi tidak diketahui1
Achmad RodiansyahAfiliasi tidak diketahui1
Ainul Fitria MahmudahAfiliasi tidak diketahui1
Mastika Marisahani UlfahAfiliasi tidak diketahui1
Uun RohmawatiAfiliasi tidak diketahui1
Andhika Puspito NugrohoUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Effect of Light Intensity on Growth and Primary Metabolites Content of Navicula sp.</dc:title>
	<dc:creator xml:lang="en">Mudrikah, Siti </dc:creator>
	<dc:creator xml:lang="en">Putri, Renata Adaranyssa Egistha</dc:creator>
	<dc:creator xml:lang="en">Kurnianto, Dedy</dc:creator>
	<dc:creator xml:lang="en">Rasdi, Abdurrahman Muhammad Fikri </dc:creator>
	<dc:creator xml:lang="en">Tia, Erfianti</dc:creator>
	<dc:creator xml:lang="en">Suyono, Eko Agus</dc:creator>
	<dc:subject xml:lang="en">Biomass</dc:subject>
	<dc:subject xml:lang="en">Diatom</dc:subject>
	<dc:subject xml:lang="en">Light Intensity</dc:subject>
	<dc:subject xml:lang="en">Primary Metabolite</dc:subject>
	<dc:subject xml:lang="en">Navicula sp.</dc:subject>
	<dc:description xml:lang="en">Global energy demand is steadily rising, necessitating the exploration of sustainable alternatives. Microalgae, such as Navicula sp., offer a promising solution. This study investigated the impact of light intensity on the growth, and biochemical composition (carbohydrates, lipids, proteins, and carotenoids) of Navicula sp. Cultures were grown in f/2 medium under four light intensity treatments: 2100 lux (control), 3500 lux, 4500 lux, and 5500 lux. Cell growth was measured through optical density analysis. Carbohydrate, lipid, protein, and carotenoid assays were performed using phenol-sulfuric acid, Bligh and Dyer, Bradford, and spectrophotometric methods. Maximum growth was observed on the eighth day of cultivation at 4500 lux light intensity. The best specific growth rate was achieved at 5500 lux. The highest biomass, carbohydrate, lipid, and protein productivity were attained at 5500 lux (24.65 mg/mL, 0.374 mg/mL/day, 0.399±0.204 mg/mL/day, and 0.025±0.018 mg/mL/day). Conversely, the highest carotenoid productivity was recorded at 2100 lux, 0.278±0.085 mg/mL/day. By those means, the increase in light intensity was positively correlated with microalgal cell growth, biomass, carbohydrate content, and carbohydrate, lipid, and protein productivity. However, a negative correlation was found with carotenoid pigment productivity. From this research, it is evident that a light intensity of 5500 lux offers a feasible option for the industrial-scale cultivation of Navicula sp. microalgae for biomass, carbohydrate, and protein production, while 2100 lux is more suitable for carotenoid production. This research compares the findings to previous studies, emphasizing the unique way Navicula sp. responds to different light levels.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-12-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/13899</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v16i3.13899</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 16 No. 3 (2024): December 2024; 498-507</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v16i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/13899/2747</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>