Melola Discovery

← Melola Discovery

Jurnal

Selection of Potential Lignin-Degrading Bacteria and Fungi Isolates from the Gunung Lumut and Sungai Wain in East Kalimantan

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · hlm. 156–168

DOI
10.15294/biosaintifika.v17i1.14755
ISSN
2338-7610, 2085-191X
Terbit
2025-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

The enzymes laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) are ligninolytic enzyme groups that have a crucial role in degrading lignin compounds. These complex components are abundant in plant biomass. This research aims to isolate and characterize microorganisms producing the enzymes Lac, MnP, and LiP from leaf litter, rhizosphere soil, and sediment in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan. The bacterial and fungal isolates obtained were cultured on NA and PDA media enriched with 0.05% guaiacol as a substrate. Initial selection was carried out based on the colony growth rate and the formation of a brownish discoloration zone, which indicates ligninolytic enzyme activity. The superior isolates were then tested for extracellular enzyme activity quantitatively using a UV-Vis spectrophotometer. Among the bacterial isolates, the highest Lac activity is isolated GLS 5.1.2 (173.44 U L-1); the highest MnP activity is isolated GLS 5.3 (177.41 U L-1); and the highest LiP activity is isolated GLS 5.1.2 (1034.05 U L-1). In the group of fungi, isolate SS2 was detected with the highest Lac activity (20.66 U L-1), MnP activity (333.75 U L-1), and LiP activity (2516.13 U L-1). Biodiversity sources in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan have the potential to produce microbial isolates with unique enzymatic characteristics that have never been reported before. The resulting microbial isolate can be used as a bioactivator in composting organic waste to produce high-quality organic fertilizer.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 127 tautan kepenulisan bersama · 28 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Cenderawasih University
  • National Research and Innovation Agency
  • Universitas Sains dan Teknologi Jayapura
  • Indonesian Institute of Sciences
  • The University of Tokyo
  • Sebelas Maret University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Suharno SuharnoCenderawasih University11
Verena AgustiniCenderawasih University8
Rosye H.R. TanjungCenderawasih University5
Vita PurnamasariCenderawasih University2
Agnes Eri MaryuniCenderawasih University2
SHERLITA MAULANICenderawasih University2
ANNISA ALFAADHILAH RUHANICenderawasih University2
Suriani Br. SurbaktiCenderawasih University2
Charisma Puspa OmegaCenderawasih University2
Irma RahayuCenderawasih University2
SuharnoCenderawasih University2
Cahya Putri IrawanCenderawasih University1
Evie N. QomariahCenderawasih University1
Ira A. PutriCenderawasih University1
Sarah YigibalomCenderawasih University1
Margaretha Z. Pangau-AdamCenderawasih University1
Suharno SuharnoCenderawasih University1
Iriandi H. BoneCenderawasih University1
Lucia RunggeariCenderawasih University1
Rr. Evi D. AryuniCenderawasih University1
Eva Susanty SimaremareCenderawasih University1
Septriyanto DirgantaraCenderawasih University1
Rosye HR. TanjungCenderawasih University1
Komari KomariUniversitas Sains dan Teknologi Jayapura1
Vita PurnamasariCenderawasih University1
Puguh SujartaCenderawasih University1
Farmawaty FarmawatyCenderawasih University1
Daawia DaawiaCenderawasih University1
Bambang SuhartawanUniversitas Sains dan Teknologi Jayapura1
LISYE IRIANA ZEBUACenderawasih University1
Suharno SuharnoSebelas Maret University1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Selection of Potential Lignin-Degrading Bacteria and Fungi Isolates from the Gunung Lumut and Sungai Wain in East Kalimantan</dc:title>
	<dc:creator xml:lang="en">Ruhimat, Riki</dc:creator>
	<dc:creator xml:lang="en">Kumala Dewi, Tirta </dc:creator>
	<dc:creator xml:lang="en">Noviana, Zahra</dc:creator>
	<dc:creator xml:lang="en">Sutisna, Entis</dc:creator>
	<dc:creator xml:lang="en">Mulyani, Nani</dc:creator>
	<dc:creator xml:lang="en">Juwari, Micha</dc:creator>
	<dc:creator xml:lang="en">Sufaati, Supeni</dc:creator>
	<dc:creator xml:lang="en">Antonius, Sarjiya</dc:creator>
	<dc:creator xml:lang="en">Verchius, Eric </dc:creator>
	<dc:subject xml:lang="en">laccase</dc:subject>
	<dc:subject xml:lang="en">organic matter</dc:subject>
	<dc:subject xml:lang="en">extracellular enzymes</dc:subject>
	<dc:description xml:lang="en">The enzymes laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) are ligninolytic enzyme groups that have a crucial role in degrading lignin compounds. These complex components are abundant in plant biomass. This research aims to isolate and characterize microorganisms producing the enzymes Lac, MnP, and LiP from leaf litter, rhizosphere soil, and sediment in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan. The bacterial and fungal isolates obtained were cultured on NA and PDA media enriched with 0.05% guaiacol as a substrate. Initial selection was carried out based on the colony growth rate and the formation of a brownish discoloration zone, which indicates ligninolytic enzyme activity. The superior isolates were then tested for extracellular enzyme activity quantitatively using a UV-Vis spectrophotometer. Among the bacterial isolates, the highest Lac activity is isolated GLS 5.1.2 (173.44 U L-1); the highest MnP activity is isolated GLS 5.3 (177.41 U L-1); and the highest LiP activity is isolated GLS 5.1.2 (1034.05 U L-1). In the group of fungi, isolate SS2 was detected with the highest Lac activity (20.66 U L-1), MnP activity (333.75 U L-1), and LiP activity (2516.13 U L-1). Biodiversity sources in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan have the potential to produce microbial isolates with unique enzymatic characteristics that have never been reported before. The resulting microbial isolate can be used as a bioactivator in composting organic waste to produce high-quality organic fertilizer.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/14755</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.14755</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 156-168</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/14755/3669</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>