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Identification of Microalgae Isolates Using 18 S rRNA Markers and Testing Their Antioxidant Capacity

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · hlm. 19–30

DOI
10.15294/biosaintifika.v17i1.19919
ISSN
2338-7610, 2085-191X
Terbit
2025-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Microalgae are photoautotrophic microorganisms that synthesize bioactive compounds, including antioxidant compounds. Exploratory research on microalgae with strong antioxidant capacity as free radical scavengers is interesting and important to do. This study aims to identify and evaluate the antioxidant capacity of microalgae. Water samples were diluted with multilevel dilution. Microalgae were isolated using the streak plate method, and four microalgae isolates were successfully cultivated in vitro. Identification was carried out by phylogenetic analysis based on 18S rRNA marker gene sequences, namely Chlorella vulgaris, Desmodesmus armatus, Dictyosphaerium ehrenbergianum, and Vitreochlamys incisa. Antioxidant capacity was evaluated using three methods, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate)), and FRAP (Ferric Reducing Antioxidant Power). IC50 as the concentration required to inhibit 50% of free radicals showed that methanol extract of C. vulgaris had a strong antioxidant capacity in the ABTS test with IC50 of 81.693 ppm and the highest in the DPPH test with IC50 of 297.451 ppm. In the FRAP test, the highest antioxidant capacity was in ethanol extract of D. ehrenbergianum isolate of 74.45 mg AAE/g. The D. armatus isolate had the lowest antioxidant capacity in the ABTS, DPPH, and FRAP tests.

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  • Institut Teknologi Sepuluh Nopember
  • Universitas Airlangga
  • Bandung Institute of Technology
  • IPB University
  • Universitas Gadjah Mada
  • Prince of Songkla University
  • National Research and Innovation Agency
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Rekan penulisOrganisasiArtikel bersama
Ramadhani Eka PutraBandung Institute of Technology3
Rika RaffiudinIPB University3
RC Hidayat SoesilohadiUniversitas Gadjah Mada3
Alfiyyana Nurrahma MawardaniInstitut Teknologi Sepuluh Nopember3
Farid Kamal MuzakiInstitut Teknologi Sepuluh Nopember3
Adi Setyo PurnomoInstitut Teknologi Sepuluh Nopember3
Ni’matuzahroh Ni’matuzahrohUniversitas Airlangga3
Siska Yulia RukhmanaInstitut Teknologi Sepuluh Nopember3
Thalita MeidinaInstitut Teknologi Sepuluh Nopember3
Leonardo Pascalis Dimas Cahyo BaskoroInstitut Teknologi Sepuluh Nopember3
Belva Aria MahendraInstitut Teknologi Sepuluh Nopember1
Anisatus SolihahInstitut Teknologi Sepuluh Nopember1
Dianita IndahsariInstitut Teknologi Sepuluh Nopember1
Aulia Sunan FadilahInstitut Teknologi Sepuluh Nopember1
Wirdhatul MuslihatinInstitut Teknologi Sepuluh Nopember1
Ratchata ChokwiwatkulPrince of Songkla University1
Edy Setiti Wida UtamiUniversitas Airlangga1
Aziz PurwantoroUniversitas Gadjah Mada1
Endang SemiartiUniversitas Gadjah Mada1
FatimahNational Research and Innovation Agency1
Salamun -Afiliasi tidak diketahui1
Muhammad Fath Alhaqqi Sanis SalamyAfiliasi tidak diketahui1
Nabilatun NisaUniversitas Airlangga1
Farah Aisyah NafidiastriUniversitas Negeri Surabaya1
Rizky Danang SusetyoUniversitas Airlangga1
Hakimatul HusniyahUniversitas Airlangga1
Almando GeraldiUniversitas Airlangga1
Nina Ratna DjuitaIPB University1
Ibnul QayimIPB University1
Dwika BramastaIPB University1
Ida KinasihSunan Gunung Djati State Islamic University Bandung1
Rezha Tanu DewanggaBandung Institute of Technology1
Endang HermawanBandung Institute of Technology1
Andi Gita Maulidyah Indraswari SuhriHasanuddin University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Identification of Microalgae Isolates Using 18 S rRNA Markers and Testing Their Antioxidant Capacity</dc:title>
	<dc:creator xml:lang="en">Ermavitalini, Dini</dc:creator>
	<dc:creator xml:lang="en">Rahmahana, Ratna Syifa'a</dc:creator>
	<dc:creator xml:lang="en">Saputro, Triono Bagus</dc:creator>
	<dc:creator xml:lang="en">Rizam, Bilqis Naura Safira</dc:creator>
	<dc:creator xml:lang="en">Purnobasuki, Hery</dc:creator>
	<dc:creator xml:lang="en">Ni'matuzahroh</dc:creator>
	<dc:subject xml:lang="en">ABTS</dc:subject>
	<dc:subject xml:lang="en">Antioxidant capacity</dc:subject>
	<dc:subject xml:lang="en">DPPH</dc:subject>
	<dc:subject xml:lang="en">FRAP</dc:subject>
	<dc:subject xml:lang="en">ITS microalgae isolate</dc:subject>
	<dc:description xml:lang="en">Microalgae are photoautotrophic microorganisms that synthesize bioactive compounds, including antioxidant compounds. Exploratory research on microalgae with strong antioxidant capacity as free radical scavengers is interesting and important to do. This study aims to identify and evaluate the antioxidant capacity of microalgae. Water samples were diluted with multilevel dilution. Microalgae were isolated using the streak plate method, and four microalgae isolates were successfully cultivated in vitro. Identification was carried out by phylogenetic analysis based on 18S rRNA marker gene sequences, namely Chlorella vulgaris, Desmodesmus armatus, Dictyosphaerium ehrenbergianum, and Vitreochlamys incisa. Antioxidant capacity was evaluated using three methods, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate)), and FRAP (Ferric Reducing Antioxidant Power). IC50 as the concentration required to inhibit 50% of free radicals showed that methanol extract of C. vulgaris had a strong antioxidant capacity in the ABTS test with IC50 of 81.693 ppm and the highest in the DPPH test with IC50 of 297.451 ppm. In the FRAP test, the highest antioxidant capacity was in ethanol extract of D. ehrenbergianum isolate of 74.45 mg AAE/g. The D. armatus isolate had the lowest antioxidant capacity in the ABTS, DPPH, and FRAP tests.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/19919</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.19919</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 19-30</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/19919/3609</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>