Melola Discovery

← Melola Discovery

Jurnal

Microbial Diversity in Hot Spring Soil Microbiome

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · hlm. 80–90

DOI
10.15294/biosaintifika.v17i1.20869
ISSN
2338-7610, 2085-191X
Terbit
2025-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Hot springs are natural habitats for thermophilic and hyperthermophilic microorganisms with optimal growth temperatures. This study was conducted using a metagenomic approach to analyze the microbial communities of Nglimut hot spring soils, Mount Ungaran. This study used an exploratory observational method. A total of ten samples of hot spring sediment soil were taken purposively in Nglimut hot spring, Mount Ungaran. A total of 600 grams of hot spring sediment soil samples were used for chemical analysis and 50 grams of samples for metagenomic analysis based on 16S rRNA V3-V4 gene marker regions. The total microbiota sequences analyzed in this study were 103,889 OTUs, consisting of 98,603 Bacteria OTUs and 5,286 Archaea OTUs. At the phylum level, all DNA sequences of soil microbiota bacteria identified 53 phyla, dominated by Proteobacteria (19.42%), Chloroflexi (17.21%), Nitrospirota (13.76%), Bacteroidota (7.67%) and Firmicutes (7.33%). At the order level, 305 bacterial orders were found, dominated by Nitrospirales (12.35%), Burkholderiales (7.99%), Rhizobiales (5.77%), Ignavibacteriales (4.06), and Anaerolineales (3.92%). A total of nine archaea phyla were identified in the hot spring soil, dominated by Crenarchaeota (85.13%), Nanoarchaeota (7.13%) and Halobacterota (5.58%). At the order level, 15 archaea orders were identified, dominated by Nitrosopumilales (64.08%), Nitrososphaerales (14.39%), Woesearchaeales (7.24%), Bathyarchaeia (6.15%), and Methanosarciniales (5.07%). The bacteria that dominate the soil of hot springs are bacteria that can survive at high temperatures (thermophilic) and are able to utilize sulfur. The presence of archaea in hot spring soils helps increase the activity of sulfate-reducing bacteria in sulfur-containing soils.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 102 tautan kepenulisan bersama · 4 lintas organisasi · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • State University of Semarang
  • University of Szeged
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ari YuniastutiState University of Semarang9
Novi Ratna DewiState University of Semarang6
Retno Sri IswariState University of Semarang6
Siti Harnina BintariAfiliasi tidak diketahui3
Didik Huswo UtomoAfiliasi tidak diketahui3
Muchamad DafipAfiliasi tidak diketahui3
Wulan ChristijantiAfiliasi tidak diketahui2
Nugrahaningsih WHAfiliasi tidak diketahui2
Wiwi IsnaeniState University of Semarang2
Priyantini WidiyaningrumAfiliasi tidak diketahui2
Aditya MariantiAfiliasi tidak diketahui2
Ani RusilowatiState University of Semarang2
Fitri Arum SasiAfiliasi tidak diketahui2
Tiara Dwi WulandariAfiliasi tidak diketahui2
Erna Noor SavitriState University of Semarang2
Enni Suwarsi RahayuState University of Semarang2
Melissa Salma DarmawanState University of Semarang2
Fitri DaeniAfiliasi tidak diketahui2
Lili Astuti IsnaeniAfiliasi tidak diketahui2
Yuni WijayantiAfiliasi tidak diketahui2
MAGGY T. SUHARTONOAfiliasi tidak diketahui2
Retno D. SoejoedonoAfiliasi tidak diketahui2
Fidia FibrianaState University of Semarang2
Prima Gita IslamiAfiliasi tidak diketahui1
Prima Gita Islami IslamiAfiliasi tidak diketahui1
Eva Ulfiatus ShofiaAfiliasi tidak diketahui1
Anggun Try SetyaningsihAfiliasi tidak diketahui1
Bagus Hammam FalahAfiliasi tidak diketahui1
Nana Kariada MartutiAfiliasi tidak diketahui1
Retno PurwantiAfiliasi tidak diketahui1
- UmarudinAfiliasi tidak diketahui1
Rheza Alfy YuliantoAfiliasi tidak diketahui1
Yuliana AstrianaAfiliasi tidak diketahui1
Arum KartikadewiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Microbial Diversity in Hot Spring Soil Microbiome</dc:title>
	<dc:creator xml:lang="en">Susanti, R.</dc:creator>
	<dc:creator xml:lang="en">Listiaji, Prasetyo</dc:creator>
	<dc:creator xml:lang="en">Mukaromah, Riska Laila </dc:creator>
	<dc:creator xml:lang="en">Nisa, Farah Fitrotun </dc:creator>
	<dc:creator xml:lang="en">Kenarni, Naina Rizki </dc:creator>
	<dc:creator xml:lang="en">Jaya, Adi Franata </dc:creator>
	<dc:subject xml:lang="en">diversity</dc:subject>
	<dc:subject xml:lang="en">metagenomic</dc:subject>
	<dc:subject xml:lang="en">Mount Ungaran</dc:subject>
	<dc:subject xml:lang="en">Nglimut hot spring</dc:subject>
	<dc:subject xml:lang="en">soil</dc:subject>
	<dc:description xml:lang="en">Hot springs are natural habitats for thermophilic and hyperthermophilic microorganisms with optimal growth temperatures. This study was conducted using a metagenomic approach to analyze the microbial communities of Nglimut hot spring soils, Mount Ungaran. This study used an exploratory observational method. A total of ten samples of hot spring sediment soil were taken purposively in Nglimut hot spring, Mount Ungaran. A total of 600 grams of hot spring sediment soil samples were used for chemical analysis and 50 grams of samples for metagenomic analysis based on 16S rRNA V3-V4 gene marker regions. The total microbiota sequences analyzed in this study were 103,889 OTUs, consisting of 98,603 Bacteria OTUs and 5,286 Archaea OTUs. At the phylum level, all DNA sequences of soil microbiota bacteria identified 53 phyla, dominated by Proteobacteria (19.42%), Chloroflexi (17.21%), Nitrospirota (13.76%), Bacteroidota (7.67%) and Firmicutes (7.33%). At the order level, 305 bacterial orders were found, dominated by Nitrospirales (12.35%), Burkholderiales (7.99%), Rhizobiales (5.77%), Ignavibacteriales (4.06), and Anaerolineales (3.92%). A total of nine archaea phyla were identified in the hot spring soil, dominated by Crenarchaeota (85.13%), Nanoarchaeota (7.13%) and Halobacterota (5.58%). At the order level, 15 archaea orders were identified, dominated by Nitrosopumilales (64.08%), Nitrososphaerales (14.39%), Woesearchaeales (7.24%), Bathyarchaeia (6.15%), and Methanosarciniales (5.07%). The bacteria that dominate the soil of hot springs are bacteria that can survive at high temperatures (thermophilic) and are able to utilize sulfur. The presence of archaea in hot spring soils helps increase the activity of sulfate-reducing bacteria in sulfur-containing soils.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20869</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.20869</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 80-90</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20869/3614</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>