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First Record of Free-Living Nematode Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida) From Indonesia

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · hlm. 41–49

DOI
10.15294/biosaintifika.v17i1.20990
ISSN
2338-7610, 2085-191X
Terbit
2025-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.

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  • Universitas Jember
  • Universitas Borneo Tarakan
  • Universitas Nurul Jadid
  • Siliwangi University
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Rekan penulisOrganisasiArtikel bersama
Fariz Kustiawan AlfarisyAfiliasi tidak diketahui4
Mohammad HoesainAfiliasi tidak diketahui3
Muh. AdiwenaUniversitas Borneo Tarakan3
Sri WahyuningsihAfiliasi tidak diketahui2
Rachmi MasnilahAfiliasi tidak diketahui1
Suhartiningsih Dwi NurchayantiAfiliasi tidak diketahui1
Dwi Tirta SariAfiliasi tidak diketahui1
Farchan Mushal Al RamadhanAfiliasi tidak diketahui1
RismayaniAfiliasi tidak diketahui1
Sri Ita TariganAfiliasi tidak diketahui1
Diana PutriAfiliasi tidak diketahui1
Deril Indana DamayantiAfiliasi tidak diketahui1
Ahmed Ibrahim Alrashid YousifAfiliasi tidak diketahui1
Sigit PrastowoAfiliasi tidak diketahui1
Ali WafaAfiliasi tidak diketahui1
HARDIAN SUSILO ADDYAfiliasi tidak diketahui1
Widi Kusuma WardhaniAfiliasi tidak diketahui1
Zul Fauzi Nugroho HadiAfiliasi tidak diketahui1
Nanang Tri HaryadiAfiliasi tidak diketahui1
Anita FirnandaAfiliasi tidak diketahui1
Sofia SofiaUniversitas Nurul Jadid1
Yuli HariyatiUniversitas Jember1
Sudarko SudarkoUniversitas Jember1
Mochammad Wildan JadmikoAfiliasi tidak diketahui1
Khaerani NurlaelitaAfiliasi tidak diketahui1
Dyah Retno AnggrainiAfiliasi tidak diketahui1
Dyah Ayu SavitriAfiliasi tidak diketahui1
Siswoyo SoekarnoAfiliasi tidak diketahui1
Farchan Mushaf Al RamadhaniAfiliasi tidak diketahui1
Dwi ApriyaniSiliwangi University1
M MardhianaAfiliasi tidak diketahui1
Rachmi MasnillahAfiliasi tidak diketahui1
Ival Oktavian Nurtian BudiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">First Record of Free-Living Nematode Ironus dentifurcatus Argo &amp; Heyns, 1972 (Ironidae: Enoplida) From Indonesia</dc:title>
	<dc:creator xml:lang="en">Pradana, Ankardiansyah Pandu</dc:creator>
	<dc:creator xml:lang="en">Yoshiga, Toyoshi</dc:creator>
	<dc:subject xml:lang="en">18S rDNA </dc:subject>
	<dc:subject xml:lang="en">agroecosystem</dc:subject>
	<dc:subject xml:lang="en">rhizosphere</dc:subject>
	<dc:subject xml:lang="en">morphometry</dc:subject>
	<dc:subject xml:lang="en">SSU (Small Subunit)</dc:subject>
	<dc:description xml:lang="en">Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.20990</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 41-49</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990/3611</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>