Melola Discovery

← Melola Discovery

Jurnal

Optimizing Canang Flower Waste Extract for Staining Fasciolopsis buski Eggs: An Enhancement of the Kato-Katz Method

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 2 (2025) · hlm. 227–236

DOI
10.15294/biosaintifika.v17i2.22829
ISSN
2338-7610, 2085-191X
Terbit
2025-08-01
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Fasciolopsis buski is a parasitic helminth that can infect humans. Diagnosing helminthiasis can be confirmed through fecal testing utilizing the Kato-Katz method. The Kato-Katz method employs methylene blue staining, which poses environmental hazards due to its carcinogenic characteristics. An alternative to staining is the use of a canang, a common Balinese item used for prayers. The utilized waste canang flowers were Tagetes erecta, Impatiens balsamina (red), and Impatiens balsamina (purple). The research began with an extraction procedure that involved cutting the flowers into small pieces, resulting in approximately 500 grams of fragments. The flower components were macerated in two liters of 96% ethanol for five days. Additionally, cellophane immersion of the extracted findings was performed. The findings indicated that each sample from the three treatment groups (T1, 1%; T2, 2%; and T3, 3%) and the two control groups. Sub-analysis testing evaluated the quantity of helminth eggs, quantified as eggs per gram of feces via field-of-view observation. The T3 (3%) exhibited results that were not statistically significantly different (P>0.05) from the positive control group. The T3 (3%) test provides the most favorable and optimal results as a substitute for methylene blue in microscopic staining evaluations.

Kata kunci

Jaringan kolaborasi para penulis

36 penulis · 152 tautan kepenulisan bersama · 17 lintas organisasi

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Universitas Jambi
  • Universitas Pendidikan Ganesha
  • Okayama University
  • STIKES Bina Usada Bali
  • Universitas Gadjah Mada
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Made Kurnia Widiastuti GiriUniversitas Pendidikan Ganesha7
Komang Kirana Ardhia PramestiUniversitas Pendidikan Ganesha6
Nyoman Intan Permatahati WigunaUniversitas Pendidikan Ganesha6
Indriyani IndriyaniUniversitas Jambi4
Mursalin MursalinUniversitas Jambi3
Addion NizoriUniversitas Jambi3
Dian WulansariUniversitas Jambi2
Silvi Leila RahmiUniversitas Jambi2
Mursyid MursyidUniversitas Jambi2
Ni Kadek Diah PurnamayantiUniversitas Pendidikan Ganesha1
Made Satya Nugraha GautamaSTIKES Bina Usada Bali1
Ni Luh Putu Pranena SastriAfiliasi tidak diketahui1
I Wayan MuderawanAfiliasi tidak diketahui1
Mahalul AzamAfiliasi tidak diketahui1
Made Mahaguna PutraAfiliasi tidak diketahui1
Rahmania AmbarikaAfiliasi tidak diketahui1
Cheng Hwee MingAfiliasi tidak diketahui1
Ika GusrianiUniversitas Jambi1
Rahayu SusenoUniversitas Jambi1
Surhaini SurhainiUniversitas Jambi1
Lavlinesia LavlinesiaUniversitas Jambi1
Ulyarti UlyartiUniversitas Jambi1
Dian WulansariAfiliasi tidak diketahui1
Nur HasnahUniversitas Jambi1
Ayi NurfitryahAfiliasi tidak diketahui1
Nurfaijah NurfaijahUniversitas Jambi1
Fitri TafziUniversitas Jambi1
Ajib DiptyanusaUniversitas Gadjah Mada1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Optimizing Canang Flower Waste Extract for Staining Fasciolopsis buski Eggs: An Enhancement of the Kato-Katz Method</dc:title>
	<dc:creator xml:lang="en">I Komang Tri Yasa Widnyana</dc:creator>
	<dc:creator xml:lang="en">Kadek Indira Maheswari</dc:creator>
	<dc:creator xml:lang="en">Putu Sathiya Adi Janendra</dc:creator>
	<dc:creator xml:lang="en">Dewa Gede Putra Mahayana</dc:creator>
	<dc:creator xml:lang="en">Indra Dwisaputra</dc:creator>
	<dc:creator xml:lang="en">Made Bayu Permasutha</dc:creator>
	<dc:creator xml:lang="en">Irma Rahmayani</dc:creator>
	<dc:creator xml:lang="en">Pasala, Metamalik </dc:creator>
	<dc:subject xml:lang="en">Fasciolopsis buski</dc:subject>
	<dc:subject xml:lang="en">Helminthiasis</dc:subject>
	<dc:subject xml:lang="en">Impatiens balsamina</dc:subject>
	<dc:subject xml:lang="en">Methylene blue</dc:subject>
	<dc:subject xml:lang="en">Tagetes erecta</dc:subject>
	<dc:description xml:lang="en">Fasciolopsis buski is a parasitic helminth that can infect humans. Diagnosing helminthiasis can be confirmed through fecal testing utilizing the Kato-Katz method.  The Kato-Katz method employs methylene blue staining, which poses environmental hazards due to its carcinogenic characteristics. An alternative to staining is the use of a canang, a common Balinese item used for prayers. The utilized waste canang flowers were Tagetes erecta, Impatiens balsamina (red), and Impatiens balsamina (purple). The research began with an extraction procedure that involved cutting the flowers into small pieces, resulting in approximately 500 grams of fragments. The flower components were macerated in two liters of 96% ethanol for five days. Additionally, cellophane immersion of the extracted findings was performed. The findings indicated that each sample from the three treatment groups (T1, 1%; T2, 2%; and T3, 3%) and the two control groups.  Sub-analysis testing evaluated the quantity of helminth eggs, quantified as eggs per gram of feces via field-of-view observation. The T3 (3%) exhibited results that were not statistically significantly different (P&amp;gt;0.05) from the positive control group. The T3 (3%) test provides the most favorable and optimal results as a substitute for methylene blue in microscopic staining evaluations.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-08-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/22829</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i2.22829</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 2 (2025): August 2025; 227-236</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/22829/5710</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>