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Morpho-Physiological, ISSR-Based Molecular Characterization of Gamma-Irradiated Soybean Under Waterlogging Stress

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · hlm. 550–560

DOI
10.15294/biosaintifika.v17i3.31511
ISSN
2338-7610, 2085-191X
Terbit
2025-11-18
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Soybeans (Glycine max) are Indonesia's third-most important food crop after rice and maize. However, because of its susceptibility to waterlogging, its productivity decreases. Gamma-ray irradiation has been used to create soybean varieties that are resistant to waterlogging. This study investigates the genetic variation and morpho-physiological traits of irradiated Grobogan soybean varieties under waterlogging stress. Soybean seeds were exposed to gamma-ray doses of 0 Gy, 25 Gy, 50 Gy, 75 Gy, and 100 Gy, then the soybean plants were submerged in water at concentrations of 0%, 100%, 150%, 200%, and 250%. The study's morpho-physiological parameters include seed weight, pod count, plant height, chlorophyll content, and seed viability. The results imply that varying waterlogging levels and radiation dosages show varied responses. All irradiation doses showed optimal growth in the control treatment, whereas 75 Gy and 100 Gy under 250% waterlogging conditions showed the most severe growth inhibition. To evaluate genetic variation among the irradiated soybean variants, five ISSR markers were employed. Because of its high PIC value of 0.391, marker ISSR-1 was found to be the most effective in detecting polymorphisms. ISSR-1 is a promising marker for assessing genetic diversity in irradiated soybean plants, according to the results. These results are valuable for the development of soybean breeding strategies targeting improved tolerance to waterlogging stress.

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  • Institut Teknologi Sepuluh Nopember
  • Universitas Airlangga
  • Universitas Gadjah Mada
  • Prince of Songkla University
  • Bandung Institute of Technology
  • IPB University
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Rekan penulisOrganisasiArtikel bersama
Hery PurnobasukiUniversitas Airlangga3
Dini ErmavitaliniInstitut Teknologi Sepuluh Nopember3
Ni'matuzahrohAfiliasi tidak diketahui1
Ratna Syifa’a RahmahanaInstitut Teknologi Sepuluh Nopember1
Bilqis Naura Safira Rizam RizamBandung Institute of Technology1
Aziz PurwantoroUniversitas Gadjah Mada1
Endang SemiartiUniversitas Gadjah Mada1
Alfiyyana Nurrahma MawardaniInstitut Teknologi Sepuluh Nopember1
Farid Kamal MuzakiInstitut Teknologi Sepuluh Nopember1
Adi Setyo PurnomoInstitut Teknologi Sepuluh Nopember1
. SUMARYONOAfiliasi tidak diketahui1
Diah RatnadewiIPB University1
Ni’matuzahroh Ni’matuzahrohUniversitas Airlangga1
Siska Yulia RukhmanaInstitut Teknologi Sepuluh Nopember1
Thalita MeidinaInstitut Teknologi Sepuluh Nopember1
Leonardo Pascalis Dimas Cahyo BaskoroInstitut Teknologi Sepuluh Nopember1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Morpho-Physiological, ISSR-Based Molecular Characterization of Gamma-Irradiated Soybean Under Waterlogging Stress</dc:title>
	<dc:creator xml:lang="en">Solihah, Anisatus</dc:creator>
	<dc:creator xml:lang="en">Indahsari, Dianita</dc:creator>
	<dc:creator xml:lang="en">Mahendra, Belva Aria</dc:creator>
	<dc:creator xml:lang="en">Fadilah, Aulia Sunan</dc:creator>
	<dc:creator xml:lang="en">Muslihatin, Wirdhatul</dc:creator>
	<dc:creator xml:lang="en">Chokwiwatkul, Ratchata </dc:creator>
	<dc:creator xml:lang="en">Saputro, Triono Bagus</dc:creator>
	<dc:subject xml:lang="en">Morpho-physiological</dc:subject>
	<dc:subject xml:lang="en">Glycine max</dc:subject>
	<dc:subject xml:lang="en">ISSR</dc:subject>
	<dc:subject xml:lang="en">Waterlogging</dc:subject>
	<dc:subject xml:lang="en">Irradiation</dc:subject>
	<dc:description xml:lang="en">Soybeans (Glycine max) are Indonesia's third-most important food crop after rice and maize. However, because of its susceptibility to waterlogging, its productivity decreases. Gamma-ray irradiation has been used to create soybean varieties that are resistant to waterlogging. This study investigates the genetic variation and morpho-physiological traits of irradiated Grobogan soybean varieties under waterlogging stress. Soybean seeds were exposed to gamma-ray doses of 0 Gy, 25 Gy, 50 Gy, 75 Gy, and 100 Gy, then the soybean plants were submerged in water at concentrations of 0%, 100%, 150%, 200%, and 250%. The study's morpho-physiological parameters include seed weight, pod count, plant height, chlorophyll content, and seed viability. The results imply that varying waterlogging levels and radiation dosages show varied responses. All irradiation doses showed optimal growth in the control treatment, whereas 75 Gy and 100 Gy under 250% waterlogging conditions showed the most severe growth inhibition. To evaluate genetic variation among the irradiated soybean variants, five ISSR markers were employed. Because of its high PIC value of 0.391, marker ISSR-1 was found to be the most effective in detecting polymorphisms. ISSR-1 is a promising marker for assessing genetic diversity in irradiated soybean plants, according to the results. These results are valuable for the development of soybean breeding strategies targeting improved tolerance to waterlogging stress.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/31511</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.31511</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 550-560</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/31511/7308</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>