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Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · pp. 423–433

DOI
10.15294/biosaintifika.v17i3.33383
ISSN
2338-7610, 2085-191X
Published
2025-11-18
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

The conservation of Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth. The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf. Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.

Keywords

Collaboration network of the authors

40 authors · 85 co-authorship links · 6 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Muria Kudus University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Mukh SyaifudinAffiliation unknown2
Krispinus Kedati PukanState University of Semarang2
Talitha WidiatningrumAffiliation unknown2
Yermia Dita Kris DAffiliation unknown2
Muhammad Abdul JabarAffiliation unknown2
Siti MasfuahMuria Kudus University1
Ani RusilowatiState University of Semarang1
Fina FakhriyahMuria Kudus University1
Mila RoysaMuria Kudus University1
Wiyanto WiyantoState University of Semarang1
Parmin ParminState University of Semarang1
E. CahyonoState University of Semarang1
Sholihatur RohmahAffiliation unknown1
Atiqah BahrunnisaAffiliation unknown1
Siti RosidahAffiliation unknown1
Khusniyyatul MunaAffiliation unknown1
Queen K. A MarthaniAffiliation unknown1
Enni S. RahayuAffiliation unknown1
Laila Nur Hidayah HidayahAffiliation unknown1
Surya DiantinaAffiliation unknown1
Nugroho Edi KartijonoState University of Semarang1
Erlangga Dwi AndikaAffiliation unknown1
Ikhsana Nuri AstitiAffiliation unknown1
Sri Wahyu PurnamiAffiliation unknown1
Yanti LusiyantiAffiliation unknown1
Nur WijawatiAffiliation unknown1
Fajar MusafaAffiliation unknown1
Khoirul MukhtarAffiliation unknown1
Y. Ulung AnggraitoAffiliation unknown1
Saiful RidloState University of Semarang1
Laily MilatuzzahrohAffiliation unknown1
Wahyu Nilam CahyatiAffiliation unknown1
Koriatun WakidahAffiliation unknown1
Mega Rifqi Ainun NajibAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium</dc:title>
	<dc:creator xml:lang="en">Rahayu, Enni Suwarsi </dc:creator>
	<dc:creator xml:lang="en">Habibah, Noor Aini </dc:creator>
	<dc:creator xml:lang="en">Anggraito, Yustinus Ulung </dc:creator>
	<dc:creator xml:lang="en">Melati, Dinda </dc:creator>
	<dc:creator xml:lang="en">Sinaga, Na Dame Maulibulung </dc:creator>
	<dc:creator xml:lang="en">Pa’ee, Furzani </dc:creator>
	<dc:subject xml:lang="en">acclimatization chamber, chitosan, leaf growth, mixed medium, Vanda floresensis</dc:subject>
	<dc:description xml:lang="en">The conservation of  Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth.  The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf.  Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/33383</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.33383</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 423-433</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/33383/7113</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
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