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Antibacterial Activity and Salinity Tolerance of Endophytic Bacteria Isolated from Marine Macroalgae

Biosaintifika: Journal of Biology & Biology Education · Vol. 18 No. 2 (2026) · hlm. 371–380

DOI
10.15294/biosaintifika.v18i2.35980
ISSN
2338-7610, 2085-191X
Terbit
2026-08-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Marine organisms, especially macroalgae, are unique microhabitats for endophytic bacteria that have the potential to provide unique microhabitats for endophytic bacteria that can produce bioactive compounds with antimicrobial activity. However, the exploration of endophytic bacteria from marine macroalgae, especially those directly associated with host tissues, remains relatively limited. This study aims to isolate endophytic bacteria from various macroalgae and explore the antibacterial potential and salinity resistance of these endophytic isolates. This study aims to identify the potential isolates that produce antibacterial compounds at the molecular level. The antibacterial activity was qualitatively evaluated using the Kirby–Bauer disk diffusion method. The salinity tolerance of the bacterial isolates was assessed by growing them on media with varying NaCl concentrations. Molecular identification of the isolates was performed through 16S rRNA gene amplification and sequence analysis. The results showed that isolate H3 had the highest antibacterial activity against Bacillus subtillis and Staphylococcus aureus, with inhibition zones of 28.9 + 2.3 mm and 26.2 + 4.36 mm, respectively. Isolate R1A showed the highest inhibitory activity on S. aureus and Escherichia coli, with inhibition zones of 25 + 1.08 mm and 35 + 2.9 mm. Salinity resistance test results showed that isolate R1A was able to grow well at a 10% (w/v) NaCl concentration. Molecular identification results with 16S rRNA gene analysis showed that isolate H3 has similarities with the species Lysinibacillus fusiformis, isolate R1A has similarities with Bacillus mojavensis, and R2C has similarities with Bacillus stercosis. This study suggests that endophytic bacteria from macroalgae represent promising and sustainable sources of novel antibacterial agents for pathogen control in high-salinity aquaculture systems. This study aligns with SDG 3 and SDG 14 by supporting human health initiatives and the sustainable utilization of marine biodiversity.

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  • Hasanuddin University
  • Sebelas Maret University
  • IPB University
  • National Research and Innovation Agency
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Rekan penulisOrganisasiArtikel bersama
Antonius SuwantoAfiliasi tidak diketahui2
Slamet SantosaHasanuddin University2
Harlita HarlitaAfiliasi tidak diketahui2
Meti IndrowatiAfiliasi tidak diketahui2
Qorisha Lutfia PramesellyAfiliasi tidak diketahui1
Bowo SugihartoAfiliasi tidak diketahui1
Sri DwiastutiSebelas Maret University1
Nuraini AnnisaAfiliasi tidak diketahui1
LISTYA UTAMI KARMAWANAfiliasi tidak diketahui1
Delia AgustinaAfiliasi tidak diketahui1
Widyah BudinartaAfiliasi tidak diketahui1
Anastasia Aliesa HermosaningtyasAfiliasi tidak diketahui1
Muhammad Ghildan Arifudin RafifAfiliasi tidak diketahui1
Cahya PrihatnaAfiliasi tidak diketahui1
Aris Tri WahyudiIPB University1
Anja MeryandiniAfiliasi tidak diketahui1
Abdjad Asih NawangsihAfiliasi tidak diketahui1
Yulin LestariAfiliasi tidak diketahui1
Dewi Puspita SariAfiliasi tidak diketahui1
Sri Martina WiraswatiAfiliasi tidak diketahui1
Vera PermatasariNational Research and Innovation Agency1
Indri YatiAfiliasi tidak diketahui1
Gian PrimahanaAfiliasi tidak diketahui1
Anggie Meilinda ZienithaAfiliasi tidak diketahui1
Meisi AnggrainiAfiliasi tidak diketahui1
Lisa RositaAfiliasi tidak diketahui1
Muhamad Eka PrastyaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Antibacterial Activity and Salinity Tolerance of Endophytic Bacteria Isolated from Marine Macroalgae</dc:title>
	<dc:creator xml:lang="en">Fatmawati, Umi</dc:creator>
	<dc:creator xml:lang="en">Faturrahim, Muhammad Ilham</dc:creator>
	<dc:creator xml:lang="en">Purnamasari, Maria Indah </dc:creator>
	<dc:subject xml:lang="en">endophytes</dc:subject>
	<dc:subject xml:lang="en">macroalgae</dc:subject>
	<dc:subject xml:lang="en">antibacterial</dc:subject>
	<dc:subject xml:lang="en">salinity</dc:subject>
	<dc:description xml:lang="en">Marine organisms, especially macroalgae, are unique microhabitats for endophytic bacteria that have the potential to provide unique microhabitats for endophytic bacteria that can produce bioactive compounds with antimicrobial activity.  However, the exploration of endophytic bacteria from marine macroalgae, especially those directly associated with host tissues, remains relatively limited. This study aims to isolate endophytic bacteria from various macroalgae and explore the antibacterial potential and salinity resistance of these endophytic isolates. This study aims to identify the potential isolates that produce antibacterial compounds at the molecular level. The antibacterial activity was qualitatively evaluated using the Kirby–Bauer disk diffusion method. The salinity tolerance of the bacterial isolates was assessed by growing them on media with varying NaCl concentrations. Molecular identification of the isolates was performed through 16S rRNA gene amplification and sequence analysis. The results showed that isolate H3 had the highest antibacterial activity against Bacillus subtillis and Staphylococcus aureus, with inhibition zones of 28.9 + 2.3 mm and 26.2 + 4.36 mm, respectively. Isolate R1A showed the highest inhibitory activity on S. aureus and Escherichia coli, with inhibition zones of 25 + 1.08 mm and 35 + 2.9 mm. Salinity resistance test results showed that isolate R1A was able to grow well at a 10% (w/v) NaCl concentration. Molecular identification results with 16S rRNA gene analysis showed that isolate H3 has similarities with the species Lysinibacillus fusiformis, isolate R1A has similarities with Bacillus mojavensis, and R2C has similarities with Bacillus stercosis. This study suggests that endophytic bacteria from macroalgae represent promising and sustainable sources of novel antibacterial agents for pathogen control in high-salinity aquaculture systems. This study aligns with SDG 3 and SDG 14 by supporting human health initiatives and the sustainable utilization of marine biodiversity.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2026-08-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/35980</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v18i2.35980</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 18 No. 2 (2026): August 2026; 371-380</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v18i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/35980/11242</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>