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Effect of BAP and Osmotic Regulators on the Germination of Synthetic Seeds of Sugarcane (Saccharum officinarum)

Biosaintifika: Journal of Biology & Biology Education · Vol. 18 No. 2 (2026) · hlm. 353–361

DOI
10.15294/biosaintifika.v18i2.37607
ISSN
2338-7610, 2085-191X
Terbit
2026-08-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Sugarcane is the raw material for sugar. Limited availability of planting materials has led to a decline in sugar production. The development of synthetic sugarcane seeds offers an alternative for large-scale propagation with long-term storage. This study aims to evaluate the effect of BAP and osmotic regulators on the germination and storage capacity of synthetic sugarcane seeds. The synthetic seeds were produced using the encapsulation technique, whereby basal shoots of the NXI 1-3 sugarcane variety were inserted into 3% sodium alginate and treated with various concentrations of BAP (0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, 2 ppm), mannitol (0%, 1%, 2%, 3%), and PEG (0%, 1%, 2%, 3%) and then dripped into a 100 mM CaCl2. The results showed that BAP at a concentration of 1.5 ppm provided the best response, with the fastest germination time (4.6 days) and the highest germination percentage (100%). Among the osmotic regulators, 3% mannitol produced the longest germination time (21.75 days), with seed viability of 85% and germination percentage of 60%, demonstrating its effectiveness in extending seed storage life. BAP increases seed viability and growth performance, while mannitol effectively maintains seed dormancy so that it can be used to maintain sugarcane seed availability. This study also supports the achievement of the SDGs, particularly SDG 2 (Zero Hunger) through increased seed production to ensure sugarcane seed availability, and SDG 9 (Industry, Innovation, and Infrastructure) through the application of encapsulation methods as a biotechnology innovation to increase seed production.

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  • Universitas Jember
  • National Research and Innovation Agency
  • Kyungpook National University
  • Sriwijaya University
  • University of Surabaya
  • Universitas Gadjah Mada
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
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Rekan penulisOrganisasiArtikel bersama
Didik Pudji RestantoUniversitas Jember4
Mohammad Nur KhozinUniversitas Jember2
Yusuf Dary AhnafUniversitas Jember2
IryonoAfiliasi tidak diketahui2
Bambang SugihartoUniversitas Jember2
Popy Hartatie HardjoUniversity of Surabaya2
Mohammad Candra PrayogaUniversitas Jember2
Sigit SoeparjonoUniversitas Jember2
UsmadiAfiliasi tidak diketahui1
Illia Seldon MagfirohAfiliasi tidak diketahui1
Laily Ilman WiduriSriwijaya University1
Subekah Nawa KartikasariUniversitas Jember1
Yuli WitonoUniversitas Jember1
Umar SantosoUniversitas Gadjah Mada1
Mohammad UbaidillahUniversitas Jember1
Nabila Nur Aisyah Al AyyubiUniversitas Jember1
Rendryana Aulia Nur KhofifaUniversitas Jember1
Mukhamad Su’udiUniversitas Jember1
Fuad Bahrul UlumUniversitas Jember1
Dwi SetyatiUniversitas Jember1
Dyah Retno WulandariNational Research and Innovation Agency1
Siti Nurul AfidahUniversitas Jember1
Inyana Dwi AgustienUniversitas Jember1
Wahyu Indra Duwi FanataUniversitas Jember1
Syafira Fatihatul HusnaUniversitas Jember1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en">Effect of BAP and Osmotic Regulators on the Germination of Synthetic Seeds of Sugarcane (Saccharum officinarum)</dc:title>
	<dc:creator xml:lang="en">Dewanti, Parawita</dc:creator>
	<dc:creator xml:lang="en">Handoyo, Tri </dc:creator>
	<dc:creator xml:lang="en">Numalasari, Numalasari</dc:creator>
	<dc:creator xml:lang="en">Shahro, Falin Fatimatus </dc:creator>
	<dc:creator xml:lang="en">Khalilah, Fakhriyah Nur</dc:creator>
	<dc:creator xml:lang="en">Maharani, Hayati Aulia </dc:creator>
	<dc:creator xml:lang="en">Kim, Kyung-Min </dc:creator>
	<dc:subject xml:lang="en">BAP</dc:subject>
	<dc:subject xml:lang="en">mannitol</dc:subject>
	<dc:subject xml:lang="en">PEG</dc:subject>
	<dc:subject xml:lang="en">synthetic seed</dc:subject>
	<dc:subject xml:lang="en">sugarcane</dc:subject>
	<dc:description xml:lang="en">Sugarcane is the raw material for sugar. Limited availability of planting materials has led to a decline in sugar production. The development of synthetic sugarcane seeds offers an alternative for large-scale propagation with long-term storage. This study aims to evaluate the effect of BAP and osmotic regulators on the germination and storage capacity of synthetic sugarcane seeds. The synthetic seeds were produced using the encapsulation technique, whereby basal shoots of the NXI 1-3 sugarcane variety were inserted into 3% sodium alginate and treated with various concentrations of BAP (0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, 2 ppm), mannitol (0%, 1%, 2%, 3%), and PEG (0%, 1%, 2%, 3%) and then dripped into a 100 mM CaCl2. The results showed that BAP at a concentration of 1.5 ppm provided the best response, with the fastest germination time (4.6 days) and the highest germination percentage (100%). Among the osmotic regulators, 3% mannitol produced the longest germination time (21.75 days), with seed viability of 85% and germination percentage of 60%, demonstrating its effectiveness in extending seed storage life. BAP increases seed viability and growth performance, while mannitol effectively maintains seed dormancy so that it can be used to maintain sugarcane seed availability. This study also supports the achievement of the SDGs, particularly SDG 2 (Zero Hunger) through increased seed production to ensure sugarcane seed availability, and SDG 9 (Industry, Innovation, and Infrastructure) through the application of encapsulation methods as a biotechnology innovation to increase seed production.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2026-08-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/37607</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v18i2.37607</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 18 No. 2 (2026): August 2026; 353-361</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v18i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/37607/11184</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>