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hpmA Gene as a Detection Method of Proteus mirabilis Bacteria using real-time Polymerase Chain Reaction

Jurnal Kesehatan Masyarakat · Vol. 21 No. 2 (2025) · hlm. 272–280

DOI
10.15294/kemas.v21i2.16214
ISSN
2355-3596, 1858-1196
Terbit
2025-10-03
Penerbit
Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)
Bahasa
eng

Abstrak

Proteus mirabilis is pathogenic bacteria that can cause gastrointestinal infections, bacteremia, and Urinary Tract Infections (UTI). Therefore, it is necessary to have a fast, sensitive, specific, and accurate detection method to identify Proteus mirabilis. This study aims to determine the confirmation, specificity, and sensitivity test of the hpmA gene primer to detect Proteus mirabilis quick and precise using the real-time Polymerase Chain Reaction method. Gradient Polymerase Chain Reaction results showed the hpmA primer has an amplicons length of 195 bp and the optimum annealing temperature at 60°C. The primer pair produced Ct value of 10.40±0.18 and showed one peak in the melting curve with Tm value of 81.84°C±0.02 by real-time PCR. Furthermore, the hpmA primer was also able to distinguish target and non-target bacteria based on the difference in Ct and Tm value formed. Based on these results, the concentration of bacterial DNA that can be detected by primers reached 3.2 pg/μL, equivalent to the concentration of target bacteria that can be detected by primers is 10.24×102 CFU. In the next step, hmpA primer will be developed to detect Proteus mirabilis in artificial contaminated samples using real-time PCR.

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  • State University of Jakarta
  • Arnold Palmer Hospital for Children
  • Universitas Airlangga
  • City of Scientific Research and Technological Applications
  • Universitas Jambi
  • University of Brawijaya
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Rekan penulisOrganisasiArtikel bersama
Fera KurniadewiState University of Jakarta20
Irma Ratna KartikaState University of Jakarta18
Vira SaamiaUniversitas Airlangga18
Hesham Ali El EnshasyCity of Scientific Research and Technological Applications16
Dandy Akbar JuliansyahState University of Jakarta13
Adinda Myra Amalia PutriState University of Jakarta12
Atikah Nur RahmawatiState University of Jakarta11
I Made WiranathaAfiliasi tidak diketahui11
Gusti Angieta PutriState University of Jakarta9
Tiara FahrizaState University of Jakarta7
Ayu BerkahingrumState University of Jakarta7
Dalia SukmawatiState University of Jakarta7
Maharanianska AzzahraState University of Jakarta7
Irvan MaulanaState University of Jakarta7
GraceAfiliasi tidak diketahui5
Ananda Indah Putri SihombingState University of Jakarta5
Puan Aqila AzizahState University of Jakarta5
Royna Rahma MusieState University of Jakarta5
Helzi AngelinaState University of Jakarta5
Sri RahayuUniversity of Brawijaya4
Rosita GIo AnggraeniState University of Jakarta4
Ismaya KrisdawatiState University of Jakarta4
Rara SeruniState University of Jakarta2
JohannesAfiliasi tidak diketahui1
Winda AmaliaAfiliasi tidak diketahui1
Novita Eka SariUniversitas Jambi1
Futi Kusuma HatiState University of Jakarta1
Nur Fadillah Adelia AzahraState University of Jakarta1
Fadhila Shofi AlbanaState University of Jakarta1
Afifah AisyafitriState University of Jakarta1
Dema Griseldis AurelliaState University of Jakarta1
Siti MunawaohState University of Jakarta1
Siti MunawarohState University of Jakarta1
Tri SusantiState University of Jakarta1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/kemas/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">hpmA Gene as a Detection Method of Proteus mirabilis Bacteria using real-time Polymerase Chain Reaction</dc:title>
	<dc:creator xml:lang="en">Nurjayadi, Muktiningsih</dc:creator>
	<dc:creator xml:lang="en">Putri, Gladys Indira</dc:creator>
	<dc:creator xml:lang="en">Fitriyanti, Anisa</dc:creator>
	<dc:creator xml:lang="en">Declan, Jefferson Lynford </dc:creator>
	<dc:creator xml:lang="en">Novitasari</dc:creator>
	<dc:creator xml:lang="en">Abomoelak, Bassam </dc:creator>
	<dc:subject xml:lang="en">Pathogenic bacteria</dc:subject>
	<dc:subject xml:lang="en">Detection method</dc:subject>
	<dc:subject xml:lang="en">real-time Polymerase Chain Reaction</dc:subject>
	<dc:subject xml:lang="en">Proteus mirabilis</dc:subject>
	<dc:subject xml:lang="en">hpmA gene</dc:subject>
	<dc:description xml:lang="en">Proteus mirabilis is pathogenic bacteria that can cause gastrointestinal infections, bacteremia, and Urinary Tract Infections (UTI). Therefore, it is necessary to have a fast, sensitive, specific, and accurate detection method to identify Proteus mirabilis. This study aims to determine the confirmation, specificity, and sensitivity test of the hpmA gene primer to detect Proteus mirabilis quick and precise using the real-time Polymerase Chain Reaction method. Gradient Polymerase Chain Reaction results showed the hpmA primer has an amplicons length of 195 bp and the optimum annealing temperature at 60°C. The primer pair produced Ct value of 10.40±0.18 and showed one peak in the melting curve with Tm value of 81.84°C±0.02 by real-time PCR. Furthermore, the hpmA primer was also able to distinguish target and non-target bacteria based on the difference in Ct and Tm value formed. Based on these results, the concentration of bacterial DNA that can be detected by primers reached 3.2 pg/μL, equivalent to the concentration of target bacteria that can be detected by primers is 10.24×102 CFU. In the next step, hmpA primer will be developed to detect Proteus mirabilis in artificial contaminated samples using real-time PCR.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)</dc:publisher>
	<dc:date>2025-10-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/kemas/article/view/16214</dc:identifier>
	<dc:identifier>10.15294/kemas.v21i2.16214</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kesehatan Masyarakat; Vol. 21 No. 2 (2025); 272-280</dc:source>
	<dc:source>2355-3596</dc:source>
	<dc:source>1858-1196</dc:source>
	<dc:source>10.15294/kemas.v21i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/kemas/article/view/16214/6280</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Jurnal Kesehatan Masyarakat</dc:rights>
</oai_dc:dc>