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Ultrasound Assisted Ethanolic Extraction of Ipomoea reptans Poir Leaves Antioxidant Activities, Total Phenolic and Flavonoids

Sainteknol : Jurnal Sains dan Teknologi · Vol. 22 No. 2 (2024) · hlm. 57–64

DOI
10.15294/sainteknol.v22i2.5331
ISSN
2527-3604, 0216-4566
Terbit
2024-12-31
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Ipomoea reptans Poir leaves contain phenolic and flavonoid compounds that can reduce free radicals and prevent diseases such as cardiovascular disease, coronary heart disease, and cancer. The drawback of conventional extraction is that it takes a long time, so a shorter extraction method is needed, one of which is the ultrasonic method. This study aims to test the antioxidant activity of ethanol extract of Ipomoea reptans Poir leaves extracted by ultrasonic method and determine the total phenolic and flavonoid content. Ipomoea reptans Poir leaves were extracted using the ultrasonic method at 50oC for 30 minutes with 70% ethanol solvent to obtain ethanol extract of Ipomoea reptans Poir leaves. Antioxidant activity test of ethanol extract of Ipomoea reptans Poir leaves using DPPH method and vitamin C as a comparison. Determination of total phenolic content using gallic acid with a maximum wavelength of 745.20 nm and operating time of 120 minutes. Determination of total flavonoid content using quercetin with a wavelength of 439.60 nm and operating time of 30 minutes. The results showed that the antioxidant activity of Ipomoea reptans Poir leaves obtained an IC50 value of 40.62 ppm (very strong). Total phenolic and flavonoids were 78.33 mgGAE/g extract and 1.03 mgQE/g extract.

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  • Universitas Wahid Hasyim
  • National Research and Innovation Agency
  • State University of Semarang
  • Universiti Sains Malaysia
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Rekan penulisOrganisasiArtikel bersama
Muhammad Eka PrastyaNational Research and Innovation Agency1
Faris HermawanNational Research and Innovation Agency1
Ririn Lispita WulandariUniversitas Wahid Hasyim1
Minarti MinartiNational Research and Innovation Agency1
Nik Nur Syazni Nik Mohamed KamalUniversiti Sains Malaysia1
Hasna Marwa AnnazwaState University of Semarang1
Sorta Dwi Margaretta NababanState University of Semarang1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/sainteknol/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Ultrasound Assisted Ethanolic Extraction of Ipomoea reptans Poir Leaves Antioxidant Activities, Total Phenolic and Flavonoids</dc:title>
	<dc:creator xml:lang="en">Arum, Nabila Setri Sekar </dc:creator>
	<dc:creator xml:lang="en">Puspitasari, Anita Dwi </dc:creator>
	<dc:subject xml:lang="en">Antioxidant, Phenolics, Flavonoids, Ultrasound extraction, Ipomoea reptans Poirs</dc:subject>
	<dc:description xml:lang="en">Ipomoea reptans Poir leaves contain phenolic and flavonoid compounds that can reduce free radicals and prevent diseases such as cardiovascular disease, coronary heart disease, and cancer. The drawback of conventional extraction is that it takes a long time, so a shorter extraction method is needed, one of which is the ultrasonic method. This study aims to test the antioxidant activity of ethanol extract of Ipomoea reptans Poir leaves extracted by ultrasonic method and determine the total phenolic and flavonoid content. Ipomoea reptans Poir leaves were extracted using the ultrasonic method at 50oC for 30 minutes with 70% ethanol solvent to obtain ethanol extract of Ipomoea reptans Poir leaves. Antioxidant activity test of ethanol extract of Ipomoea reptans Poir leaves using DPPH method and vitamin C as a comparison. Determination of total phenolic content using gallic acid with a maximum wavelength of 745.20 nm and operating time of 120 minutes. Determination of total flavonoid content using quercetin with a wavelength of 439.60 nm and operating time of 30 minutes. The results showed that the antioxidant activity of Ipomoea reptans Poir leaves obtained an IC50 value of 40.62 ppm (very strong). Total phenolic and flavonoids were 78.33 mgGAE/g extract and 1.03 mgQE/g extract.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/sainteknol/article/view/5331</dc:identifier>
	<dc:identifier>10.15294/sainteknol.v22i2.5331</dc:identifier>
	<dc:source xml:lang="en">Sainteknol : Jurnal Sains dan Teknologi; Vol. 22 No. 2 (2024): December 2024; 57-64</dc:source>
	<dc:source>2527-3604</dc:source>
	<dc:source>0216-4566</dc:source>
	<dc:source>10.15294/sainteknol.v22i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/sainteknol/article/view/5331/2710</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Sainteknol : Jurnal Sains dan Teknologi</dc:rights>
</oai_dc:dc>