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PENGARUH PEMBERIAN BERBAGAI KONSENTRASI ZPT 2,4-D (2,4-DICHLOROPHENOXYACETIC ACID) DAN BAP TERHADAP EKSPLAN DAUN DUKU KUMPEH SECARA IN VITRO

Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian · Vol. 9 No. 1 (2026) · hlm. 55–62

DOI
10.22437/agroecotania.v9i1.59045
ISSN
2621-2854, 2621-2846
Terbit
2026-07-25
Penerbit
Jambi University, Fakultas Pertanian, Program Studi Agroekoteknologi
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

The duku plant (Lansium domesticum Corr.) is a horticultural commodity originating from Southeast Asia. Duku Kumpeh is a national leading variety from Jambi Province which is seriously threatened due to the increasingly widespread attack of stem cancer. Tissue culture is an effort made to make plant parts (roots, shoots, other plant tissues) grow intact (perfect) in aseptic conditions. The research was carried out at the Biotechnology and Plant Breeding Laboratory, Faculty of Agriculture, University of Jambi. The research was carried out for 3 months from February to April 2026. This study employed a factorial randomised block design (RBD) comprising two factors: the first factor was the concentration of 2,4-D (0; 0.25; 0.5; 0.75; and 1 ppm) and the second factor was the concentration of BAP (0 and 1 ppm). A total of 10 treatments were obtained and replicated three times based on leaf groups or sections (base, middle and tip of the leaf), resulting in 30 experimental units. Each experimental unit consisted of 4 bottles, including 2 sample bottles, resulting in a total of 120 bottles, each containing a single leaf explant. The explants were cultured for 8 weeks after inoculation (MSK). The results of the study indicate that there is no interaction between the concentrations of the plant growth regulators 2,4-D and BAP and the variables of callus formation time and the percentage of callus-forming explants in Duku Kumpeh leaf explants. The application of various concentrations of 2,4-D alone was able to induce callus formation in Duku Kumpeh leaf explants, but BAP alone was not yet able to induce callus formation in Duku Kumpeh leaf explants. The application of 2,4-D at concentrations ranging from 0.25 to 1 ppm, combined with a BAP concentration of 1 ppm, is crucial for callus formation on Duku Kumpeh leaf explants.

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  • Universitas Jambi
  • Universitas Gadjah Mada
  • Bandung Institute of Technology
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Rekan penulisOrganisasiArtikel bersama
Evita EvitaUniversitas Jambi10
Trias NovitaUniversitas Jambi9
Gusniwati GusniwatiUniversitas Jambi5
Elis KartikaUniversitas Jambi4
Ardiyaningsih Puji LestariUniversitas Jambi3
Trias NovitaUniversitas Jambi3
Neliyati NeliyatiUniversitas Jambi2
Herni Dwinta PebriantiUniversitas Jambi2
Elly Indra SwariUniversitas Jambi2
Miranti Sari FitrianiUniversitas Jambi2
Syamsurizal SyamsurizalUniversitas Jambi1
Elisma ElismaUniversitas Jambi1
Diah Tri UtamiUniversitas Jambi1
Aries Bagus SasongkoUniversitas Gadjah Mada1
Beni Hendro PrabowoUniversitas Gadjah Mada1
Muhammad Teguh SatriaAfiliasi tidak diketahui1
Nerty SoverdaUniversitas Jambi1
Astra MigandiUniversitas Jambi1
Eliyanti EliyantiUniversitas Jambi1
Olya AprindaUniversitas Jambi1
Hendra Edi GunawanBandung Institute of Technology1
Ida SetiyawatiUniversitas Jambi1
Widya AliviaUniversitas Jambi1
P.Lestari ArdiyaningsihUniversitas Jambi1
Jul AndayaniUniversitas Jambi1
Rizky Ayu HardiyantiUniversitas Jambi1
Hamzah HamzahUniversitas Jambi1
Windi Putri RahayuAfiliasi tidak diketahui1
Tiara BerlianiAfiliasi tidak diketahui1
Rajjitha HandayaniAfiliasi tidak diketahui1
Beben SalahudinAfiliasi tidak diketahui1
Liz Yanti AndriyaniAfiliasi tidak diketahui1
Eka Ramona PutriAfiliasi tidak diketahui1
Hariestya ViarecoUniversitas Jambi1
Rainiyati RainiyatiUniversitas Jambi1
Ahmad RiduanUniversitas Jambi1
Sarah Fiebrina HeraningsihUniversitas Jambi1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/Agroecotania/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">PENGARUH PEMBERIAN BERBAGAI KONSENTRASI ZPT 2,4-D (2,4-DICHLOROPHENOXYACETIC ACID)   DAN BAP TERHADAP EKSPLAN DAUN DUKU KUMPEH SECARA IN VITRO</dc:title>
	<dc:creator xml:lang="en">Tobing, Daniel Trisantoso Lumban</dc:creator>
	<dc:creator xml:lang="en">Lizawati, Lizawati</dc:creator>
	<dc:creator xml:lang="en">Jasminarni, Jasminarni</dc:creator>
	<dc:subject xml:lang="en">Duku Kumpeh</dc:subject>
	<dc:subject xml:lang="en">2,4-D, BAP</dc:subject>
	<dc:subject xml:lang="en">leaf explants</dc:subject>
	<dc:subject xml:lang="en">in vitro</dc:subject>
	<dc:description xml:lang="en">The duku plant (Lansium domesticum Corr.) is a horticultural commodity originating from Southeast Asia. Duku Kumpeh is a national leading variety from Jambi Province which is seriously threatened due to the increasingly widespread attack of stem cancer. Tissue culture is an effort made to make plant parts (roots, shoots, other plant tissues) grow intact (perfect) in aseptic conditions. The research was carried out at the Biotechnology and Plant Breeding Laboratory, Faculty of Agriculture, University of Jambi. The research was carried out for 3 months from February to April 2026. This study employed a factorial randomised block design (RBD) comprising two factors: the first factor was the concentration of 2,4-D (0; 0.25; 0.5; 0.75; and 1 ppm) and the second factor was the concentration of BAP (0 and 1 ppm). A total of 10 treatments were obtained and replicated three times based on leaf groups or sections (base, middle and tip of the leaf), resulting in 30 experimental units. Each experimental unit consisted of 4 bottles, including 2 sample bottles, resulting in a total of 120 bottles, each containing a single leaf explant. The explants were cultured for 8 weeks after inoculation (MSK). The results of the study indicate that there is no interaction between the concentrations of the plant growth regulators 2,4-D and BAP and the variables of callus formation time and the percentage of callus-forming explants in Duku Kumpeh leaf explants. The application of various concentrations of 2,4-D alone was able to induce callus formation in Duku Kumpeh leaf explants, but BAP alone was not yet able to induce callus formation in Duku Kumpeh leaf explants. The application of 2,4-D at concentrations ranging from 0.25 to 1 ppm, combined with a BAP concentration of 1 ppm, is crucial for callus formation on Duku Kumpeh leaf explants.</dc:description>
	<dc:publisher xml:lang="en">Jambi University, Fakultas Pertanian, Program Studi Agroekoteknologi</dc:publisher>
	<dc:date>2026-07-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/Agroecotania/article/view/59045</dc:identifier>
	<dc:identifier>10.22437/agroecotania.v9i1.59045</dc:identifier>
	<dc:source xml:lang="en">Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian; Vol. 9 No. 1 (2026): Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian; 55-62</dc:source>
	<dc:source>2621-2854</dc:source>
	<dc:source>2621-2846</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/Agroecotania/article/view/59045/25103</dc:relation>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>