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Analysis of quercetin content as an anti-inflammatory agent in Akasia leaf extract (Acacia auriculiformis) using the HPLC method

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · hlm. 410–415

DOI
10.22437/proca.v1i2.50263
Terbit
2025-11-26
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Background: Inflammation is a natural defense response, characterized by five classic symptoms, namely swelling, heat, redness, pain, and impaired organ function. However, uncontrolled inflammation can trigger various chronic diseases, so effective and safe anti-inflammatory agents are needed. One natural compound that has this potential is quercetin, a flavonoid with antioxidant and anti-inflammatory activities through inhibition of cyclooxygenase (COX) and lipoxygenase (LOX) enzymes, as well as modulation of the NF-κB transcription factor. Acacia auriculiformis leaves are known to contain quercetin and other bioactive compounds, so they have the potential to be developed as a source of natural anti-inflammatory agents. Objective: This study aims to analyze the quercetin content in ethanol extract of Acacia leaves using high-performance liquid chromatography (HPLC). Methods: Extraction was performed by maceration using 96% ethanol. Acacia leaf simplicia weighing 300 grams was successfully extracted and produced a thick extract of 30 ml with a characteristic dark green-brown color. Then, it was analyzed using HPLC based on validation parameters of LOD (0.1172 mg/L) and LOQ (0.3906 mg/L). Results: The quercetin content was found to be below the detection limit (<0.1172 mg/L), making quantitative determination unfeasible. Conclusion: The results suggest that quercetin is present in very low concentrations or possibly in a conjugated form. Further phytochemical and GC-MS analyses are required to confirm the presence and activity of other flavonoids contributing to the anti-inflammatory potential of Acacia leaf extract.

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Esa Indah AyudiaAfiliasi tidak diketahui8
Afifah AmatullahAfiliasi tidak diketahui7
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Ahmad Thohir HidayatAfiliasi tidak diketahui4
Mirna Marhami IskandarAfiliasi tidak diketahui3
Mara Imam Taufiq SiregarUniversitas Jambi2
Hasna DewiAfiliasi tidak diketahui2
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Rita HalimUniversitas Jambi1
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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Analysis of quercetin content as an anti-inflammatory agent in Akasia leaf extract (Acacia auriculiformis) using the HPLC method</dc:title>
	<dc:creator xml:lang="en">Asty, Zahra Frizki</dc:creator>
	<dc:creator xml:lang="en">Delfira, Annissa</dc:creator>
	<dc:creator xml:lang="en">Hardiningsih, Denok Tri</dc:creator>
	<dc:creator xml:lang="en">Wijayanti, Zilzikridini</dc:creator>
	<dc:subject xml:lang="en">Quercetin</dc:subject>
	<dc:subject xml:lang="en">Acacia auriculiformis</dc:subject>
	<dc:subject xml:lang="en">HPLC</dc:subject>
	<dc:subject xml:lang="en">Flavonoid</dc:subject>
	<dc:subject xml:lang="en">Anti-inflammatory</dc:subject>
	<dc:description xml:lang="en">Background: Inflammation is a natural defense response, characterized by five classic symptoms, namely swelling, heat, redness, pain, and impaired organ function. However, uncontrolled inflammation can trigger various chronic diseases, so effective and safe anti-inflammatory agents are needed. One natural compound that has this potential is quercetin, a flavonoid with antioxidant and anti-inflammatory activities through inhibition of cyclooxygenase (COX) and lipoxygenase (LOX) enzymes, as well as modulation of the NF-κB transcription factor. Acacia auriculiformis leaves are known to contain quercetin and other bioactive compounds, so they have the potential to be developed as a source of natural anti-inflammatory agents. Objective: This study aims to analyze the quercetin content in ethanol extract of Acacia leaves using high-performance liquid chromatography (HPLC). Methods: Extraction was performed by maceration using 96% ethanol. Acacia leaf simplicia weighing 300 grams was successfully extracted and produced a thick extract of 30 ml with a characteristic dark green-brown color. Then, it was analyzed using HPLC based on validation parameters of LOD (0.1172 mg/L) and LOQ (0.3906 mg/L). Results: The quercetin content was found to be below the detection limit (&amp;lt;0.1172 mg/L), making quantitative determination unfeasible. Conclusion: The results suggest that quercetin is present in very low concentrations or possibly in a conjugated form. Further phytochemical and GC-MS analyses are required to confirm the presence and activity of other flavonoids contributing to the anti-inflammatory potential of Acacia leaf extract.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50263</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50263</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 410-415</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50263/22596</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Zahra Frizki Asty, Annissa Delfira, Denok Tri Hardiningsih, Zilzikridini Wijayanti</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>