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Qualitative confirmation of flavonoids in Moringa oleifera leaf extract and evaluation of antioxidant potential

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · hlm. 503–508

DOI
10.22437/proca.v1i2.50311
Terbit
2025-11-27
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Background: Moringa oleifera Lam. leaves are widely recognized as a medicinal plant and a high-nutrition source, with their biological activities supported by the content of phytochemical compounds. The Flavonoid group is the main class of polyphenol compounds known to confer potent antioxidant activity. Objective: This study aimed to verify the qualitative presence of flavonoids in the Moringa oleifera leaf extract and evaluate the associated free-radical scavenging capacity. Methods: Bioactive compounds were extracted using a suitable polar solvent, specifically 96% ethanol. The qualitative test for Flavonoids was performed using the Shinoda method, which employs Magnesium powder (Mg) and concentrated Hydrochloric Acid (HCI) as reagents. A positive result is confirmed by a characteristic color change (e.g., orange, red, or magenta) in the solution, indicating the reduction of the flavonoid's benzopyrone nucleus. Subsequently, the extract's antioxidant activity was qualitatively evaluated in vitro using the DPPH spot test method. Results: The qualitative screening showed a clear positive reaction for the Flavonoid group, characterized by the expected color change after the addition of Mg and HCl reagents. The confirmed presence of flavonoids correlates directly with the antioxidant evaluation, which demonstrated a strong radical scavenging ability by the extract. Conclusion: The Moringa oleifera leaf extract is qualitatively proven to contain flavonoids. These findings provide scientific validation that flavonoids are significant contributors to the plant's notable antioxidant potential, supporting its development as a natural nutraceutical source.

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Zilzikridini WijayantiAfiliasi tidak diketahui9
Mara Imam Taufiq SiregarUniversitas Jambi9
Mirna Marhami IskandarAfiliasi tidak diketahui9
Tengku Arief Buana PerkasaAfiliasi tidak diketahui7
Patrick Wiliam GadingAfiliasi tidak diketahui6
Hasna DewiAfiliasi tidak diketahui6
Afifah AmatullahAfiliasi tidak diketahui5
LipinwatiAfiliasi tidak diketahui5
Ahmad Thohir HidayatAfiliasi tidak diketahui5
Dea Anenta VeonikaAfiliasi tidak diketahui5
Tia Wida Ekaputri Hz HzAfiliasi tidak diketahui5
Willy hardi MarpaungAfiliasi tidak diketahui4
Budi JustitiaAfiliasi tidak diketahui4
Raihanah SusanAfiliasi tidak diketahui4
Tia Wida Ekaputri HzAfiliasi tidak diketahui4
Rita HalimUniversitas Jambi3
Raihanah SuzanAfiliasi tidak diketahui3
Arnati WulansariAfiliasi tidak diketahui2
Evy WisudarianiAfiliasi tidak diketahui2
Anggelia PuspasariAfiliasi tidak diketahui2
Citra MaharaniAfiliasi tidak diketahui2
Nadir Putra Indra TariganAfiliasi tidak diketahui2
Widya Lawra ArrahmaAfiliasi tidak diketahui2
Syahreza Hadi JuandaAfiliasi tidak diketahui2
Tengku Irfan Wira BuanaAfiliasi tidak diketahui2
Armaidi DarmawanAfiliasi tidak diketahui2
Fadliyana EkawatyAfiliasi tidak diketahui2
HaninaAfiliasi tidak diketahui2
Erny KusdiyahAfiliasi tidak diketahui1
Wahyu Indah Dewi AuroraAfiliasi tidak diketahui1
Huntari HarahapAfiliasi tidak diketahui1
FairuzAfiliasi tidak diketahui1
MiftahurrahmaAfiliasi tidak diketahui1
Wahyu Indah AuroraAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Qualitative confirmation of flavonoids in Moringa oleifera leaf extract and evaluation of antioxidant potential</dc:title>
	<dc:creator xml:lang="en">Hardiningsih, Denok Tri</dc:creator>
	<dc:creator xml:lang="en">Hafizah</dc:creator>
	<dc:creator xml:lang="en">Asty, Zahra Frizki</dc:creator>
	<dc:creator xml:lang="en">Delfira, Annissa</dc:creator>
	<dc:creator xml:lang="en">Miftahurrahmah</dc:creator>
	<dc:creator xml:lang="en">Ayudia, Esa Indah</dc:creator>
	<dc:subject xml:lang="en">Moringa oleifera</dc:subject>
	<dc:subject xml:lang="en">flavonoids</dc:subject>
	<dc:subject xml:lang="en">antioxidant</dc:subject>
	<dc:subject xml:lang="en">shinoda test</dc:subject>
	<dc:subject xml:lang="en">qualitative screening</dc:subject>
	<dc:description xml:lang="en">Background: Moringa oleifera Lam. leaves are widely recognized as a medicinal plant and a high-nutrition source, with their biological activities supported by the content of phytochemical compounds. The Flavonoid group is the main class of polyphenol compounds known to confer potent antioxidant activity. Objective: This study aimed to verify the qualitative presence of flavonoids in the Moringa oleifera leaf extract and evaluate the associated free-radical scavenging capacity. Methods: Bioactive compounds were extracted using a suitable polar solvent, specifically 96% ethanol. The qualitative test for Flavonoids was performed using the Shinoda method, which employs Magnesium powder (Mg) and concentrated Hydrochloric Acid (HCI) as reagents. A positive result is confirmed by a characteristic color change (e.g., orange, red, or magenta) in the solution, indicating the reduction of the flavonoid's benzopyrone nucleus. Subsequently, the extract's antioxidant activity was qualitatively evaluated in vitro using the DPPH spot test method. Results: The qualitative screening showed a clear positive reaction for the Flavonoid group, characterized by the expected color change after the addition of Mg and HCl reagents. The confirmed presence of flavonoids correlates directly with the antioxidant evaluation, which demonstrated a strong radical scavenging ability by the extract. Conclusion: The Moringa oleifera leaf extract is qualitatively proven to contain flavonoids. These findings provide scientific validation that flavonoids are significant contributors to the plant's notable antioxidant potential, supporting its development as a natural nutraceutical source.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50311</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50311</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 503-508</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50311/22613</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Denok Tri Hardiningsih, Hafizah, Zahra Frizki Asty, Annissa Delfira, Miftahurrahmah, Esa Indah Ayudia</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>