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Comparative cytotoxicity and selectivity of various orchid extracts against B16F10 melanoma cells

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · hlm. 986–993

DOI
10.22437/proca.v1i2.50527
Terbit
2025-11-30
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Background: Melanoma B16F10 is an aggressive form of skin cancer that requires novel therapies. This study aimed to examine the cytotoxicity and selectivity activities of five crude methanol extracts derived from three different orchid species—Cymbidium sp. (leaves), Grammatophyllum speciosum (leaves), and Dendrobium crumenatum (leaves, pseudobulbs, and roots) against melanoma cancer cells B16F10. Methods: Cytotoxicity activities were measured using MTT assay against B16F10 (cancer) and 3T3 (normal fibroblasts) cells to determine IC50 and selectivity index (SI). Results: All extracts exhibited dose-dependent cytotoxicity. The extract of Dendrobium crumenatum pseudobulbs showed the most vigorous activity with an IC50 value against B16F10 of 168.47±2.02 µg/mL. This extract also showed the best SI of 3.79 (IC50 3T3 = 638.73±2.98 µg/mL), which indicates a promising selectivity potential compared to other plant part extracts. Conclusion: Crude methanol extract of Dendrobium crumenatum pseudobulbs is the best candidate as a selective anticancer agent for melanoma and requires further study to isolate its active compounds.

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  • Institut Agama Islam Negeri Kendari
  • Universitas Jambi
  • Universitas Teknologi Yogyakarta
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
Hendra Dhermawan SitanggangAfiliasi tidak diketahui6
guspianto guspiantoAfiliasi tidak diketahui6
Dwi NoerjoediantoAfiliasi tidak diketahui6
Rd. HalimAfiliasi tidak diketahui6
FitrianingsihAfiliasi tidak diketahui6
Indri MahariniAfiliasi tidak diketahui6
M. RidwanAfiliasi tidak diketahui5
YuliawatiAfiliasi tidak diketahui5
Adelina FitriAfiliasi tidak diketahui4
Elisma .Afiliasi tidak diketahui4
MaimumAfiliasi tidak diketahui4
Nakhlatul AisyaAfiliasi tidak diketahui3
Asparian AsparianAfiliasi tidak diketahui3
Muldiasman MuldiasmanAfiliasi tidak diketahui3
Irma HandayaniUniversitas Teknologi Yogyakarta3
Nofrans Eka SaputraAfiliasi tidak diketahui3
Syamsurizal SyamsurizalAfiliasi tidak diketahui3
Wahyudin MaguniInstitut Agama Islam Negeri Kendari2
Beti MuluInstitut Agama Islam Negeri Kendari2
Yosi OktarinaAfiliasi tidak diketahui2
Evy WisudarianiAfiliasi tidak diketahui2
Helmi Suryani NasutionAfiliasi tidak diketahui2
Jalius JaliusAfiliasi tidak diketahui2
Damris MuhammadUniversitas Jambi2
Adriani AdrianiAfiliasi tidak diketahui2
Erny KusdiyahAfiliasi tidak diketahui2
Wahyu Indah Dewi AuroraAfiliasi tidak diketahui2
Amelia Dwi FitriAfiliasi tidak diketahui2
Anggelia PuspasariAfiliasi tidak diketahui2
Willia Novita Eka RiniAfiliasi tidak diketahui2
Adzil Arsyi SabanaInstitut Agama Islam Negeri Kendari1
Eka Rizky SaputraInstitut Agama Islam Negeri Kendari1
Rahmi RahmiInstitut Agama Islam Negeri Kendari1
St NapisaInstitut Agama Islam Negeri Kendari1
Satriyani SatriyaniInstitut Agama Islam Negeri Kendari1

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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 6 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Comparative cytotoxicity and selectivity of various orchid extracts against B16F10 melanoma cells</dc:title>
	<dc:creator xml:lang="en">Syamsurizal</dc:creator>
	<dc:creator xml:lang="en">Utami, Diah Tri</dc:creator>
	<dc:creator xml:lang="en">Subandi, Andi</dc:creator>
	<dc:creator xml:lang="en">Kalsum, Ummi</dc:creator>
	<dc:creator xml:lang="en">Prabowo, Beni Hendro</dc:creator>
	<dc:subject xml:lang="en">Orchid; B16F10; Cytotoxicity; Selectivity; Dendrobium crumenatum</dc:subject>
	<dc:description xml:lang="en">Background: Melanoma B16F10 is an aggressive form of skin cancer that requires novel therapies. This study aimed to examine the cytotoxicity and selectivity activities of five crude methanol extracts derived from three different orchid species—Cymbidium sp. (leaves), Grammatophyllum speciosum (leaves), and Dendrobium crumenatum (leaves, pseudobulbs, and roots) against melanoma cancer cells B16F10. Methods: Cytotoxicity activities were measured using MTT assay against B16F10 (cancer) and 3T3 (normal fibroblasts) cells to determine IC50 and selectivity index (SI). Results: All extracts exhibited dose-dependent cytotoxicity. The extract of Dendrobium crumenatum pseudobulbs showed the most vigorous activity with an IC50 value against B16F10 of 168.47±2.02 µg/mL. This extract also showed the best SI of 3.79 (IC50 3T3 = 638.73±2.98 µg/mL), which indicates a promising selectivity potential compared to other plant part extracts. Conclusion: Crude methanol extract of Dendrobium crumenatum pseudobulbs is the best candidate as a selective anticancer agent for melanoma and requires further study to isolate its active compounds.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50527</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50527</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 986-993</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50527/22722</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Syamsurizal, Diah Tri Utami, Andi Subandi, Ummi Kalsum, Beni Hendro Prabowo</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>