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Method development and validation of Catechin in aqueous medium by using ultraviolet-visible spectrophotometer

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · hlm. 980–985

DOI
10.22437/proca.v1i2.50529
Terbit
2025-11-30
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Background: Catechin, a flavonoid compound with significant antioxidant activity, requires a reliable analytical method for its quantitative determination in pharmaceutical and research applications. Objective: In this study, a simple and rapid UV-Visible Spectrophotometry method was developed and validated for the determination of catechin dissolved in aquadest for quality control and laboratory applications. Methods: A UV-Visible spectrophotometry method was developed for the determination catechin dissolved in aquadest. The maximum wavelenght (λmax) of catechin was determined by scanning in the range of 200-400 nm. calibration curves were prepared at concentrations ranging from 20-60 ppm. method validation was carried out according to AOAC (Association of Official Analytical Chemists) guidelines, including asessment of linearity, precision, accuracy, limit of detection (LoD) and limit of quantification (LoQ). Results: Catechin showed maximum absorbantion at 275 nm. The Calibration curve exhibited excellent linearity with a correlation coefficient (R2) of 0,99. precision studies showed %RSD values 7,3%, while accuracy testing yielded recovery values between 95,72±2,42 %- 102,05±1,74%. The LoD and LoQ were calculated to be 4,16 ppm and 12,60 ppm, respectively. Conclusion: the developed UV-Vis Spectrophotometry method is simple, precise, and accurate. it is suitable for routine determination of catechin in aqueous samples and can be applied in research as well as quality control of pharmaceutical formulation.

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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Method development and validation of Catechin in aqueous medium by using ultraviolet-visible spectrophotometer</dc:title>
	<dc:creator xml:lang="en">Pratiwi, Puspa Dwi</dc:creator>
	<dc:creator xml:lang="en">Astuti, Novia Tri</dc:creator>
	<dc:creator xml:lang="en">Perawati, Santi</dc:creator>
	<dc:creator xml:lang="en">Sadli, Nurul Kamilah</dc:creator>
	<dc:creator xml:lang="en">Neldi, Vina</dc:creator>
	<dc:creator xml:lang="en">Pondawinata, Marizki</dc:creator>
	<dc:creator xml:lang="en">Musdhaliva, Dita Avika</dc:creator>
	<dc:creator xml:lang="en">Putri, Wahyu Nidia</dc:creator>
	<dc:subject xml:lang="en">catechin; validation; spectrophotometer; aqueous</dc:subject>
	<dc:description xml:lang="en">Background: Catechin, a flavonoid compound with significant antioxidant activity, requires a reliable analytical method for its quantitative determination in pharmaceutical and research applications. Objective: In this study, a simple and rapid UV-Visible Spectrophotometry method was developed and validated for the determination of catechin dissolved in aquadest for quality control and laboratory applications. Methods: A UV-Visible spectrophotometry method was developed for the determination catechin dissolved in aquadest. The maximum wavelenght (λmax) of catechin was determined by scanning in the range of 200-400 nm. calibration curves were prepared at concentrations ranging from 20-60 ppm. method validation was carried out according to AOAC (Association of Official Analytical Chemists) guidelines, including asessment of linearity, precision, accuracy, limit of detection (LoD) and limit of quantification (LoQ). Results: Catechin showed maximum absorbantion at 275 nm. The Calibration curve exhibited excellent linearity with a correlation coefficient (R2) of 0,99. precision studies showed %RSD values 7,3%, while accuracy testing yielded recovery values between 95,72±2,42 %- 102,05±1,74%. The LoD and LoQ were calculated to be 4,16 ppm and 12,60 ppm, respectively. Conclusion: the developed UV-Vis Spectrophotometry method is simple, precise, and accurate. it is suitable for routine determination of catechin in aqueous samples and can be applied in research as well as quality control of pharmaceutical formulation.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50529</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50529</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 980-985</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50529/22721</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Puspa Dwi Pratiwi, Novia Tri Astuti, Santi Perawati, Nurul Kamilah Sadli, Vina Neldi, Marizki Pondawinata, Dita Avika Musdhaliva, Wahyu Nidia Putri</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>