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Cytotoxicity of three Orchid species extracts against HeLa, MCF-7, and T47D cancer cells

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · hlm. 973–979

DOI
10.22437/proca.v1i2.50530
Terbit
2025-11-30
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Background: Cancer remains a leading cause of death globally, necessitating the search for novel therapeutic agents from natural sources. Orchids, known to produce diverse secondary metabolites, hold significant potential. Objective: This study aims to evaluate and quantitatively compare the in vitro cytotoxicity activity of crude methanol extracts from three orchid species against HeLa, MCF-7, and T47D cells using the MTT assay. Methods: This study investigated the comparative in vitro cytotoxic activity of crude methanol extracts from three native orchid species, Cymbidium sp., Grammatophyllum speciosum, and Dendrobium crumenatum, against three human cancer cell lines: HeLa (cervical), MCF-7 (breast), and T47D (breast). Cytotoxicity assessment was performed using the MTT Assay. The extracts were tested at various concentrations to determine their inhibitory concentrations IC50.Results: The results showed that the three orchid extracts exhibited varying cytotoxic effects across the tested cell lines. Significantly, the crude methanol extract of Dendrobium crumenatum showed the most promising activity, especially against MCF-7 breast cancer cells, with an IC50 value of 126.58±5.50 µg/mL. The activity against HeLa and T47D was less potent. These findings indicate that secondary metabolites in Dendrobium crumenatum have selective antiproliferative properties. Conclusion: In conclusion, the methanol extract of Dendrobium crumenatum is a potential candidate for the isolation and further identification of active compounds in developing anticancer drugs, especially for breast cancer.

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  • Universitas Halu Oleo
  • Hasanuddin University
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YuliawatiAfiliasi tidak diketahui12
Elisma .Afiliasi tidak diketahui9
MaimumAfiliasi tidak diketahui8
Ummi KalsumAfiliasi tidak diketahui5
Puspa Dwi PratiwiAfiliasi tidak diketahui4
Uce LestariAfiliasi tidak diketahui3
Muhammad SyukriUniversitas Halu Oleo3
Fathnur Sani KasmadiAfiliasi tidak diketahui3
Marizki PondawinataAfiliasi tidak diketahui3
Syamsurizal SyamsurizalAfiliasi tidak diketahui2
Riska Amalya NasutionAfiliasi tidak diketahui2
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Zahwa Syuhada FitriAfiliasi tidak diketahui1
Chessa Nasya PutriAfiliasi tidak diketahui1
Irma HandayaniHasanuddin University1
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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Cytotoxicity of three Orchid species extracts against HeLa, MCF-7, and T47D cancer cells</dc:title>
	<dc:creator xml:lang="en">Syamsurizal</dc:creator>
	<dc:creator xml:lang="en">Utami, Diah Tri</dc:creator>
	<dc:creator xml:lang="en">Fitrianingsih</dc:creator>
	<dc:creator xml:lang="en">Maharini, Indri</dc:creator>
	<dc:creator xml:lang="en">Prabowo, Beni Hendro</dc:creator>
	<dc:subject xml:lang="en">Orchid, Cytotoxicity; MTT Assay; MCF-7, Dendrobium crumenatum</dc:subject>
	<dc:description xml:lang="en">Background: Cancer remains a leading cause of death globally, necessitating the search for novel therapeutic agents from natural sources. Orchids, known to produce diverse secondary metabolites, hold significant potential. Objective: This study aims to evaluate and quantitatively compare the in vitro cytotoxicity activity of crude methanol extracts from three orchid species against HeLa, MCF-7, and T47D cells using the MTT assay. Methods: This study investigated the comparative in vitro cytotoxic activity of crude methanol extracts from three native orchid species, Cymbidium sp., Grammatophyllum speciosum, and Dendrobium crumenatum, against three human cancer cell lines: HeLa (cervical), MCF-7 (breast), and T47D (breast). Cytotoxicity assessment was performed using the MTT Assay. The extracts were tested at various concentrations to determine their inhibitory concentrations IC50.Results: The results showed that the three orchid extracts exhibited varying cytotoxic effects across the tested cell lines. Significantly, the crude methanol extract of Dendrobium crumenatum showed the most promising activity, especially against MCF-7 breast cancer cells, with an IC50 value of 126.58±5.50 µg/mL. The activity against HeLa and T47D was less potent. These findings indicate that secondary metabolites in Dendrobium crumenatum have selective antiproliferative properties. Conclusion: In conclusion, the methanol extract of Dendrobium crumenatum is a potential candidate for the isolation and further identification of active compounds in developing anticancer drugs, especially for breast cancer.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50530</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50530</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 973-979</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50530/22720</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Syamsurizal, Diah Tri Utami, Fitrianingsih, Indri Maharini, Beni Hendro Prabowo</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>