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In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA–NA Media

Proceedings Academic Universitas Jambi · Vol. 2 No. 2 (2026) · hlm. 270–278

DOI
10.22437/proca.v2i2.55580
Terbit
2026-07-15
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA-NA Media. Background: Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens are widely used biological control agents, but their combined use requires compatibility assessment to prevent antagonistic interactions in microbial consortia. Objective: This study evaluated the in vitro compatibility of T. harzianum with B. subtilis and P. fluorescens on King’s B and PDA-NA media. Methods: A dual-culture assay was conducted by observing inhibition zones and measuring the colony diameter of T. harzianum at 24 and 48 hours; percentage inhibition and Cohen’s d effect size were then calculated. Results: On King’s B medium, clear inhibition zones were observed and percentage inhibition reached 57.04% in T. harzianum x B. subtilis and 62.89% in T. harzianum x P. fluorescens at 48 hours, indicating strong antagonism. On PDA-NA medium, no inhibition zones were detected and inhibition values were lower, at 10.96% and 18.40%, respectively. Conclusion: The three microorganisms may be combined under nutrient conditions that do not strongly stimulate antagonistic metabolite production, supporting the development of multi-agent biocontrol formulations.

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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA–NA Media</dc:title>
	<dc:creator xml:lang="en">Putri Dwi</dc:creator>
	<dc:creator xml:lang="en">Abdilla, Triyuni Verza</dc:creator>
	<dc:creator xml:lang="en">Maritsa, Hasna Ul</dc:creator>
	<dc:creator xml:lang="en">Destiana, Anggari Linda</dc:creator>
	<dc:creator xml:lang="en">Bestari, Andini Vermita</dc:creator>
	<dc:creator xml:lang="en"> Safitri, Ayu Nadila</dc:creator>
	<dc:subject xml:lang="en">Trichoderma harzianum</dc:subject>
	<dc:subject xml:lang="en">Bacillus subtilis</dc:subject>
	<dc:subject xml:lang="en">Pseudomonas fluorescens</dc:subject>
	<dc:subject xml:lang="en">compatibility</dc:subject>
	<dc:subject xml:lang="en">biocontrol</dc:subject>
	<dc:description xml:lang="en">In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA-NA Media. Background: Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens are widely used biological control agents, but their combined use requires compatibility assessment to prevent antagonistic interactions in microbial consortia. Objective: This study evaluated the in vitro compatibility of T. harzianum with B. subtilis and P. fluorescens on King’s B and PDA-NA media. Methods: A dual-culture assay was conducted by observing inhibition zones and measuring the colony diameter of T. harzianum at 24 and 48 hours; percentage inhibition and Cohen’s d effect size were then calculated. Results: On King’s B medium, clear inhibition zones were observed and percentage inhibition reached 57.04% in T. harzianum x B. subtilis and 62.89% in T. harzianum x P. fluorescens at 48 hours, indicating strong antagonism. On PDA-NA medium, no inhibition zones were detected and inhibition values were lower, at 10.96% and 18.40%, respectively. Conclusion: The three microorganisms may be combined under nutrient conditions that do not strongly stimulate antagonistic metabolite production, supporting the development of multi-agent biocontrol formulations.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2026-07-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/55580</dc:identifier>
	<dc:identifier>10.22437/proca.v2i2.55580</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 2 No. 2 (2026): The 3rd Jambi International Conference On Engineering, Science and Technology (JICEST); 270-278</dc:source>
	<dc:source>10.22437/proca.v2i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/55580/25063</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 PROCA UNJA</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>