Melola Discovery

← Melola Discovery

Jurnal

Mycobacterium Tuberculosis Identification on Suspected Extra Pulmonary Tuberculosis Patients

Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan · Vol. 13 No. 1 (2025) · hlm. 10–15

DOI
10.22437/jmj.v13i1.38458
ISSN
2580-6874, 2339-269X
Terbit
2025-05-28
Penerbit
FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI
Bahasa
en

Abstrak

Background: Extra Pulmonary Tuberculosis (EPTB) is Tuberculosis infection in organs other than the lungs, like pleura, lymph nodes, abdomen, genitourinary tract, skin, joints and bones, or meninges. The Diagnosis of EPTB is still challenging because symptoms of EPTB is vary, location EPTB is an inaccessible site, and paucibacillary smear. We wanted to know the Mycobacterium tuberculosis identification using the qPCR method on samples of EPTB suspects. Methods: This research was a descriptive research with laboratory experiments. Subjects in this study were all cases of suspected EPTB with clinically diagnosed, thoraks radiographs, colonoscopy, and formalin fixed paraffin embedded (FFPE). DNA extraction was carried out according to PureLink® Genomic dna extraction kit instructions. The PCR volume 20 ul with 2x QuantiNova SYBR Green PCR Kit, M.tuberculosis Primer IS 6110, and DNA Sample. QPCR temperatures was done with predenaturation at 95 °C for 3 minutes, and followed 45 cycles, consist of denaturation at 95 °C for 15 seconds, annealing at 60 °C for 30 seconds dan extenstion at 72 °C for 30 seconds. PCR was performed with positive and negative controls, and data analysis was based on positive and negative ct controls. Results: From 30 suspect EPTB samples, there are males (56,67%), with age 36-55 yo (50%), site of samples most from gastrointestines (70%), and there are 6 samples (20%) with positive qPCR. Conclusion: qPCR methods can be used for EPTB diagnosis.

Kata kunci

Jaringan kolaborasi para penulis

40 penulis · 255 tautan kepenulisan bersama · menampilkan 40 yang paling banyak terhubung

Arahkan kursor atau ketuk sebuah nama untuk menyorot rekan penulisnya. Klik dua kali untuk membuka halaman penulis.

  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
  • Garis lebih tebal = lebih banyak artikel bersama · lingkaran lebih besar = lebih banyak artikel
Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Tia Wida EkaputriAfiliasi tidak diketahui8
Wahyu Indah Dewi AuroraAfiliasi tidak diketahui7
Esa Indah AyudiaAfiliasi tidak diketahui7
KamariyahAfiliasi tidak diketahui5
Erny KusdiyahAfiliasi tidak diketahui5
Ahmad SyauqyAfiliasi tidak diketahui5
Denok Tri HardiningsihAfiliasi tidak diketahui5
Tia Wida Ekaputri HzAfiliasi tidak diketahui5
Rita HalimAfiliasi tidak diketahui5
Fitriyanti FitriyantiAfiliasi tidak diketahui4
Huntari HarahapAfiliasi tidak diketahui4
Rina Nofri EnisAfiliasi tidak diketahui4
Armaidi DarmawanAfiliasi tidak diketahui4
HafizahAfiliasi tidak diketahui4
MiftahurrahmahAfiliasi tidak diketahui3
YuliawatiAfiliasi tidak diketahui3
Niken Irfa NastitiAfiliasi tidak diketahui3
Andika SulistiawanAfiliasi tidak diketahui3
HaninaAfiliasi tidak diketahui3
Raihanah SuzanAfiliasi tidak diketahui3
LipinwatiAfiliasi tidak diketahui3
FitriyantiAfiliasi tidak diketahui2
Siti RaudhohAfiliasi tidak diketahui2
Nyimas Natasha Ayu ShafiraAfiliasi tidak diketahui2
Nindya AryantyAfiliasi tidak diketahui2
Budi JustitiaAfiliasi tidak diketahui2
Ave Olivia RahmanAfiliasi tidak diketahui2
Amelia Dwi FitriAfiliasi tidak diketahui2
Miftahurrahmah MiftahurrahmahAfiliasi tidak diketahui2
Fairuz QuzwainAfiliasi tidak diketahui2
Maria Estela KarolinaAfiliasi tidak diketahui2

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/kedokteran/oai, pertama di-harvest 6 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Mycobacterium Tuberculosis Identification on Suspected Extra Pulmonary Tuberculosis Patients</dc:title>
	<dc:creator xml:lang="en">Lipinwati, Lipinwati</dc:creator>
	<dc:creator xml:lang="en">Hanina, Hanina</dc:creator>
	<dc:creator xml:lang="en">Wulandari, Putri Sari</dc:creator>
	<dc:creator xml:lang="en">Iskandar, Mirna Marhami</dc:creator>
	<dc:creator xml:lang="en">Istarini, Attiya</dc:creator>
	<dc:creator xml:lang="en">Miranda, Melly</dc:creator>
	<dc:creator xml:lang="en">Dewi, Hasna</dc:creator>
	<dc:creator xml:lang="en">Juniati, Rianita</dc:creator>
	<dc:creator xml:lang="en">Fairuz, Fairuz</dc:creator>
	<dc:subject xml:lang="en">EPTB</dc:subject>
	<dc:subject xml:lang="en">qPCR</dc:subject>
	<dc:subject xml:lang="en">Mycobacterium Tuberculosis</dc:subject>
	<dc:description xml:lang="en">Background: Extra Pulmonary Tuberculosis (EPTB) is Tuberculosis infection in organs other than the lungs, like pleura, lymph nodes, abdomen, genitourinary tract, skin, joints and bones, or meninges. The Diagnosis of EPTB is still challenging because symptoms of EPTB is vary, location EPTB is an inaccessible site, and paucibacillary smear. We wanted to know the Mycobacterium tuberculosis identification using the qPCR method on samples of EPTB suspects.
Methods: This research was a descriptive research with laboratory experiments. Subjects in this study were all cases of suspected EPTB with clinically diagnosed, thoraks radiographs, colonoscopy, and formalin fixed paraffin embedded (FFPE). DNA extraction was carried out according to  PureLink® Genomic dna extraction kit instructions. The PCR volume 20 ul with 2x QuantiNova SYBR Green PCR Kit, M.tuberculosis Primer IS 6110, and DNA Sample. QPCR temperatures was done with predenaturation at 95 °C for 3 minutes, and followed 45 cycles, consist of denaturation at 95 °C for 15 seconds, annealing at 60 °C for 30 seconds dan extenstion at 72 °C for 30 seconds. PCR was performed with positive and negative controls, and data analysis was based on positive and negative ct controls.
Results: From 30 suspect EPTB samples, there are males (56,67%), with age 36-55 yo (50%), site of samples most from gastrointestines (70%), and there are 6 samples (20%) with positive qPCR.
Conclusion: qPCR methods can be used for EPTB diagnosis.</dc:description>
	<dc:publisher xml:lang="en">FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI</dc:publisher>
	<dc:date>2025-05-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/kedokteran/article/view/38458</dc:identifier>
	<dc:identifier>10.22437/jmj.v13i1.38458</dc:identifier>
	<dc:source xml:lang="en">Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan; Vol. 13 No. 1 (2025): JAMBI MEDICAL JOURNAL: Jurnal Kedokteran dan Kesehatan; 10-15</dc:source>
	<dc:source>2580-6874</dc:source>
	<dc:source>2339-269X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/kedokteran/article/view/38458/21895</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan</dc:rights>
</oai_dc:dc>