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AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI N-HEKSANA KULIT BUAH KAKAO (Theobroma cacao L.)

Lantanida Journal · Vol. 13 No. 1 (2025) · pp. 72–84

DOI
10.22373/lj.v13i1.29989
ISSN
2548-9062, 2356-3133
Published
2025-06-04
Publisher
UIN AR-RANIRY
Language
eng

Abstract

This study aims to evaluate the antibacterial activity of the n-hexane extract and fraction of cocoa pod husk against Staphylococcus aureus. The antibacterial was tested using the paper disc diffusion method. The results showed that the extract inhibited bacterial with inhibition zones of 7.13 ± 0.29; 8.58 ± 0.14; 9.60 ± 0.87; and 10.97 ± 0.08 mm at concentrations of 1%, 5%, 10%, and 20%, respectively. The fractionation process produced 9 fractions and the 9th fraction showed the greatest inhibitory activity with inhibition zone diameters of 7.42 ± 0.03; 9.22 ± 0.03; 10.43 ± 0.03; and 11.83 ± 0.03 mm at concentrations of 1%; 5%; 10%; and 20% respectively. Phytochemical analysis and thin-layer chromatography (TLC) showed the presence of terpenoid and steroid compounds. Based on the antibacterial activity exhibited by the extract and fraction, this plant shows potential to be developed as a source of natural antibacterials.

Keywords

Collaboration network of the authors

30 authors · 99 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • UIN Sunan Ampel Surabaya
  • Universitas Muslim Indonesia
  • Universitas Syiah Kuala
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Muhammad ArdiansyahUIN Sunan Ampel Surabaya1
Ulva Ria IrvanAffiliation unknown1
Sulastri SulastriUniversitas Muslim Indonesia1
A HalimAffiliation unknown1
Teuku Muhammad Hary RamadhanAffiliation unknown1
Safrida SAffiliation unknown1
Irfan MustafaAffiliation unknown1
NurjanahAffiliation unknown1
Ilham MaulanaAffiliation unknown1
Tasya UlfikaAffiliation unknown1
Cut HusnaUniversitas Syiah Kuala1
Hajjul KamilAffiliation unknown1
Teuku TahlilAffiliation unknown1
Darmawati DarmawatiAffiliation unknown1
Baihaqi BaihaqiAffiliation unknown1
Eka SafitriAffiliation unknown1
Nurul IslamiAffiliation unknown1
Sagir AlvaAffiliation unknown1
Khairi SuhudAffiliation unknown1
Julinawati JulinawatiAffiliation unknown1
Syafrizal FonnaAffiliation unknown1
Ahmad Kamal ArifinAffiliation unknown1
Zulnazri ZulnazriAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ar-raniry.ac.id/index.php/lantanida/oai, first harvested 7 Nov 2026, last seen 7 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="id">AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI N-HEKSANA KULIT BUAH KAKAO (Theobroma cacao L.)</dc:title>
	<dc:creator xml:lang="id">Purnama, Agnia</dc:creator>
	<dc:creator xml:lang="id">Ginting, Binawati</dc:creator>
	<dc:creator xml:lang="id">Yahya, Mustanir</dc:creator>
	<dc:creator xml:lang="id">Afifi, Muhammad Ridho</dc:creator>
	<dc:creator xml:lang="id">Maghfirah, Ruhul</dc:creator>
	<dc:creator xml:lang="id">Farida, Mutia</dc:creator>
	<dc:creator xml:lang="id">Hamdiyati, Hamdiyati</dc:creator>
	<dc:subject xml:lang="id">Antibacterial</dc:subject>
	<dc:subject xml:lang="id">Cacao Pod Husk</dc:subject>
	<dc:subject xml:lang="id">Staphylococcus aureus</dc:subject>
	<dc:subject xml:lang="id">Theobroma cacao L.</dc:subject>
	<dc:description xml:lang="id">This study aims to evaluate the antibacterial activity of the n-hexane extract and fraction of cocoa pod husk against Staphylococcus aureus. The antibacterial was tested using the paper disc diffusion method. The results showed that the extract inhibited bacterial with inhibition zones of 7.13 ± 0.29; 8.58 ± 0.14; 9.60 ± 0.87; and 10.97 ± 0.08 mm at concentrations of 1%, 5%, 10%, and 20%, respectively. The fractionation process produced 9 fractions and the 9th fraction showed the greatest inhibitory activity with inhibition zone diameters of 7.42 ± 0.03; 9.22 ± 0.03; 10.43 ± 0.03; and 11.83 ± 0.03 mm at concentrations of 1%; 5%; 10%; and 20% respectively. Phytochemical analysis and thin-layer chromatography (TLC) showed the presence of terpenoid and steroid compounds. Based on the antibacterial activity exhibited by the extract and fraction, this plant shows potential to be developed as a source of natural antibacterials.</dc:description>
	<dc:publisher xml:lang="en">UIN AR-RANIRY</dc:publisher>
	<dc:date>2025-06-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.ar-raniry.ac.id/index.php/lantanida/article/view/29989</dc:identifier>
	<dc:identifier>10.22373/lj.v13i1.29989</dc:identifier>
	<dc:source xml:lang="en">Lantanida Journal; Vol. 13 No. 1 (2025): January-June 2025; 72-84</dc:source>
	<dc:source xml:lang="id">Lantanida Journal; Vol 13 No 1 (2025): January-June 2025; 72-84</dc:source>
	<dc:source>2548-9062</dc:source>
	<dc:source>2356-3133</dc:source>
	<dc:source>10.22373/</dc:source>
	<dc:language>eng</dc:language>
	<dc:language>ind</dc:language>
	<dc:relation>https://jurnal.ar-raniry.ac.id/index.php/lantanida/article/view/29989/pdf</dc:relation>
	<dc:relation>https://jurnal.ar-raniry.ac.id/index.php/lantanida/article/view/29989/11696</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Agnia Purnama</dc:rights>
</oai_dc:dc>