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RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA

al Kimiya: Jurnal Ilmu Kimia dan Terapan · Vol. 11 No. 2 (2024) · pp. 141–146

DOI
10.15575/ak.v11i2.39438
ISSN
2407-1927, 2407-1897
Published
2024-12-30
Publisher
Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
Language
eng

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.

Keywords

Collaboration network of the authors

21 authors · 35 co-authorship links · 2 across different organisations

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  • Hasanuddin University
  • Poltekkes Kemenkes Banjarmasin
  • Universitas Islam Negeri Sumatera Utara
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Syaikhu SyaikhuAffiliation unknown2
Syamsuddin ToahaHasanuddin University1
Kasbawati KasbawatiHasanuddin University1
Rezky NefianthiAffiliation unknown1
Yulianti HidayahAffiliation unknown1
Juliana NasutionUniversitas Islam Negeri Sumatera Utara1
Yenni Samri Juliati NasutionAffiliation unknown1
Susilawati SusilawatiAffiliation unknown1
Lenny Anwar SAffiliation unknown1
Gusti MuzainahAffiliation unknown1
Norwili NorwiliAffiliation unknown1
Abdul HelimAffiliation unknown1
Aris Sunandar SuradilagaAffiliation unknown1
Wafid Syuja' Vennovary BenevolentAffiliation unknown1
Muhammad NorhadiAffiliation unknown1
Abdul KhairPoltekkes Kemenkes Banjarmasin1
Zainatul FatmahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ak/oai, first harvested 7 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA</dc:title>
	<dc:creator>Astuti, Winni</dc:creator>
	<dc:creator>Adawiyah, Rabiatul</dc:creator>
	<dc:creator>Putri, Amanda Aulia</dc:creator>
	<dc:creator>Pratiwi, Djihan Ryn</dc:creator>
	<dc:subject xml:lang="en-US">Raw starch degrading amylase (RSDA)</dc:subject>
	<dc:subject xml:lang="en-US">Karang Mumus River</dc:subject>
	<dc:subject xml:lang="en-US">Dinitrosalicylic Acid</dc:subject>
	<dc:subject xml:lang="en-US">Klebsiella pneumoniae</dc:subject>
	<dc:description xml:lang="en-US">Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda.  RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.</dc:description>
	<dc:publisher xml:lang="en-US">Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung</dc:publisher>
	<dc:date>2024-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ak/article/view/39438</dc:identifier>
	<dc:identifier>10.15575/ak.v11i2.39438</dc:identifier>
	<dc:source xml:lang="en-US">al Kimiya: Jurnal Ilmu Kimia dan Terapan; Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan; 141-146</dc:source>
	<dc:source>2407-1927</dc:source>
	<dc:source>2407-1897</dc:source>
	<dc:source>10.15575/ak.v11i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ak/article/view/39438/12492</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Winni Astuti, Rabiatul Adawiyah, Amanda Aulia Putri, Djihan Ryn Pratiwi</dc:rights>
</oai_dc:dc>