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Penapisan aktinobakteria rhizosfer padi sebagai agens pengendali hayati Xanthomonas oryzae pv. oryzae pathogen penyebab penyakit hawar daun bakteri

Jurnal AGRO · Vol. 10 No. 1 (2023) · pp. 1–15

DOI
10.15575/19798
ISSN
2407-7933
Published
2023-07-31
Publisher
Jurusan Agroteknologi
Language
eng

Abstract

Bacterial leaf blight caused by X.o. pv. oryzae is an important disease of rice plants. Actinobacteria has potential as biological agents to control X.o. pv. oryzae because it has the ability to produce bioactive compounds. This study aimed to select actinobacteria isolates that can suppress the development of X.o. pv. oryzae and has the potential to stimulate the growth of rice plants in-planta, as well as to determine the ability of actinobacteria in producing enzymes that inhibit the development of X. oryzae pv. oryzae. The research consisted of four stages, namely: isolation, selection, characterization, and potential inhibition of actinobacterial isolates. A total of 30 isolates were successfully isolated from the rhizosphere of rice plants in three districts of West Sumatra, and as many as 25 isolates were successfully selected based on biosafety tests. The results of the in-planta test showed that 10 isolates had the ability to increase the growth and suppresed the development of bacterial leaf blight. The results of the antagonist test showed that 5 isolates inhibited of X.o. pv. oryzae by 11.66-29.66%. Five isolates were selected, namely: APRD 3I211, APRD 1I122APRP 2S121, APRP 1I121, APRP 3I212 wich capable of produce protease enzymes, cellulases, amylase, and secondary metabolites.ABSTRAKÂ Penyakit hawar daun bakteri yang disebabkan oleh Xanthomonas oryzae. pv. oryzae merupakan penyakit penting tanaman padi. Aktinobakteria memiliki potensi sebagai agens hayati untuk mengendalikan X. oryzae. pv. oryzae karena memiliki kemampuan dalam menghasilkan senyawa bioaktif. Penelitian ini bertujuan untuk menyeleksi isolat aktinobakteria yang dapat menekan perkembangan X. oryzae. pv. oryzae dan memiliki potensi dalam memacu pertumbuhan tanaman padi secara in-planta, serta mengetahui kemampuan aktinobakteria dalam menghasilkan enzim penghambat perkembangan X. oryzae. pv. oryzae. Penelitian terdiri dari empat tahap, yaitu: isolasi, seleksi, karakterisasi, dan potensi daya hambat isolat aktinobakteria. Sebanyak 30 isolat berhasil diisolasi dari rizosfer tanaman padi di tiga Kabupaten Sumatera Barat, dan sebanyak 25 isolat berhasil diseleksi berdasarkan uji keamanan hayati. Hasil uji in-planta menunjukkan 10 isolat memiliki kemampuan dalam meningkatkan pertumbuhan dan menekan perkembangan hawar daun bakteri. Hasil uji antagonis menunjukkan 5 isolat menghasilkan penghambatan terhadap X. oryzae. pv. oryzae sebesar 11,66-29,66%. Lima isolat terpilih yaitu: APRD 3I211, APRD 1I122, APRP 2S121, APRP 1I121, APRP 3I212 terbukti mampu menghasilkan enzim protease, selulase, amilase, metabolit sekunder.

Keywords

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27 authors · 67 co-authorship links

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Co-authorOrganisationJoint articles
Hasmiandy HamidAffiliation unknown7
Yaherwandi YaherwandiAffiliation unknown5
Nurbailis NurbailisAffiliation unknown3
Trimurti HabazarAffiliation unknown3
Reflin ReflinAffiliation unknown2
Zurai RestiAffiliation unknown2
Imam RifaiAffiliation unknown1
Yogie Aditya PratamaAffiliation unknown1
Muhammad Ihsan HarahapAffiliation unknown1
Febri Yani ChrismontAffiliation unknown1
Ilham WibowoAffiliation unknown1
Tifla Fitri AnnisaAffiliation unknown1
Dian Larasati MunawarAffiliation unknown1
Beny HartonoAffiliation unknown1
Muhammad MunawarAffiliation unknown1
Fadhil Pratama ApriansyahAffiliation unknown1
Darwin IndraAffiliation unknown1
Reflinaldon ReflinaldonAffiliation unknown1
Miranti MirantiAffiliation unknown1
Dewi SuhalitaAffiliation unknown1
Winarto WinartoAffiliation unknown1
Akmal JamaanAffiliation unknown1
Silva Dika RahayuAffiliation unknown1
Raflin ReflinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ja/oai, first harvested 7 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Penapisan aktinobakteria rhizosfer padi sebagai agens pengendali hayati Xanthomonas oryzae pv. oryzae pathogen penyebab penyakit hawar daun bakteri</dc:title>
	<dc:creator>Fadil, Muhammad</dc:creator>
	<dc:creator>Yanti, Yulmira</dc:creator>
	<dc:creator>Khairul, Ujang</dc:creator>
	<dc:subject xml:lang="en-US">Aktinobakteria</dc:subject>
	<dc:subject xml:lang="en-US">metabolit sekunder</dc:subject>
	<dc:subject xml:lang="en-US">Xanthomonas oryzae pv. oryzae</dc:subject>
	<dc:subject xml:lang="en-US">zona hambat</dc:subject>
	<dc:description xml:lang="en-US">Bacterial leaf blight caused by X.o. pv. oryzae is an important disease of rice plants. Actinobacteria has potential as biological agents to control X.o. pv. oryzae because it has the ability to produce bioactive compounds. This study aimed to select actinobacteria isolates that can suppress the development of X.o. pv. oryzae and has the potential to stimulate the growth of rice plants in-planta, as well as to determine the ability of actinobacteria in producing enzymes that inhibit the development of X. oryzae pv. oryzae. The research consisted of four stages, namely: isolation, selection, characterization, and potential inhibition of actinobacterial isolates. A total of 30 isolates were successfully isolated from the rhizosphere of rice plants in three districts of West Sumatra, and as many as 25 isolates were successfully selected based on biosafety tests. The results of the in-planta test showed that 10 isolates had the ability to increase the growth and suppresed the development of bacterial leaf blight. The results of the antagonist test showed that 5 isolates inhibited of X.o. pv. oryzae by 11.66-29.66%. Five isolates were selected, namely: APRD 3I211, APRD 1I122APRP 2S121, APRP 1I121, APRP 3I212 wich capable of produce protease enzymes, cellulases, amylase, and secondary metabolites.ABSTRAK Penyakit hawar daun bakteri yang disebabkan oleh Xanthomonas oryzae. pv. oryzae merupakan penyakit penting tanaman padi. Aktinobakteria memiliki potensi sebagai agens hayati untuk mengendalikan X. oryzae. pv. oryzae karena memiliki kemampuan dalam menghasilkan senyawa bioaktif. Penelitian ini bertujuan untuk menyeleksi isolat aktinobakteria yang dapat menekan perkembangan X. oryzae. pv. oryzae dan memiliki potensi dalam memacu pertumbuhan tanaman padi secara in-planta, serta mengetahui kemampuan aktinobakteria dalam menghasilkan enzim penghambat perkembangan X. oryzae. pv. oryzae. Penelitian terdiri dari empat tahap, yaitu: isolasi, seleksi, karakterisasi, dan potensi daya hambat isolat aktinobakteria. Sebanyak 30 isolat berhasil diisolasi dari rizosfer tanaman padi di tiga Kabupaten Sumatera Barat, dan sebanyak 25 isolat berhasil diseleksi berdasarkan uji keamanan hayati. Hasil uji in-planta menunjukkan 10 isolat memiliki kemampuan dalam meningkatkan pertumbuhan dan menekan perkembangan hawar daun bakteri. Hasil uji antagonis menunjukkan 5 isolat menghasilkan penghambatan terhadap X. oryzae. pv. oryzae sebesar 11,66-29,66%. Lima isolat terpilih yaitu: APRD 3I211, APRD 1I122, APRP 2S121, APRP 1I121, APRP 3I212 terbukti mampu menghasilkan enzim protease, selulase, amilase, metabolit sekunder.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Agroteknologi</dc:publisher>
	<dc:date>2023-07-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ja/article/view/19798</dc:identifier>
	<dc:identifier>10.15575/19798</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal AGRO; Vol. 10 No. 1 (2023); 1-15</dc:source>
	<dc:source>2407-7933</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ja/article/view/19798/13098</dc:relation>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ja/article/view/19798/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 yulmira yanti</dc:rights>
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