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Isolasi dan karakterisasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” dan potensinya menghambat penyakit bercak ungu Alternaria porri (ELL) CIF

Jurnal AGRO · Vol. 10 No. 2 (2023) · pp. 278–292

DOI
10.15575/28242
ISSN
2407-7933
Published
2023-12-29
Publisher
Jurusan Agroteknologi
Language
eng

Abstract

The decrease in shallot production due to Alternaria porri infection can reach 50%-70% or can even cause crop failure. One of the biological control methods to overcome this is by utilizing antagonistic microbes. The purpose of this study was to identify endophytic fungi from the rhizosphere of shallots "Lembah Palu " that have the potential to inhibit the A. porri fungus that causes purple spot disease. Research January-September 2022, the activity began with isolating endophytic fungi using the surface sterilization method, followed by characterization and identification of morphology macroscopically and microscopically, compatibility testing between isolates, and antagonism power test. The results obtained 5 isolates of endophytic fungi identified in the genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, and Trichoderma sp. A total of seven compatible interactions between endophytic fungi resulted from compatibility tests, while 3 other interactions were not compatible. The antagonism test of 5 fungal isolates against the A. porri produced an average inhibition of ≥ 75%. The HKP5 isolate (Penicillium sp) had the best inhibition of 78.96%. This indicates that endophytic fungal isolates have some potencies the potential to be developed as biological control agents in an effort to support sustainable agriculture.ABSTRAKPenurunan produksi tanaman bawang merah akibat serangan Alternaria porri dapat mencapai 50%-70% bahkan dapat menyebabkan terjadinya gagal panen. Salah satu metode pengendalian hayati untuk mengatasi hal tersebut yaitu dengan memanfaatkan mikroba yang bersifat antagonis. Tujuan dari penelitian ini untuk mengidentifikasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” yang berpotensi dalam menghambat cendawan A. porri penyebab penyakit bercak ungu. Penelitian Januari-September 2022, kegiatan diawali dengan mengisolasi cendawan endofit menggunakan metode sterilisasi permukaan, dengan perendaman sampel akar berurutan dalam 2% Natrium hipoklorit, etanol 70% dan air steril, kemudian dikulturkan dalam media Potato Dextrose Agar (PDA). Selanjutnya setelah isolat tumbuh, disubkultur kembali untuk dilakukan karakterisasi dan identifikasi morfologi secara makroskopis dan mikroskopis, pengujian kompatibilitas antarisolat serta uji daya antagonisme. Dari hasil penelitian diperoleh 5 isolat cendawan endofit yang teridentifikasi dalam genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, dan Trichoderma sp. Sebanyak tujuh interaksi kompatibel antarcendawan endofit dihasilkan dari uji kompatibilitas, sedangkan 3 interaksi lainnya tidak kompatibel. Uji daya antagonisme 5 isolat cendawan terhadap patogen A. porri menghasilkan rata-rata daya hambat ≥ 75%. Isolat HKP5 (Penicillium sp) memiliki daya hambat terbaik sebesar 78,96%. Hal ini mengindikasikan bahwa isolat cendawan endofit berpotensi untuk dikembangkan sebagai agens pengendali hayati dalam upaya mendukung pertanian berkelanjutan.

Keywords

Collaboration network of the authors

40 authors · 72 co-authorship links · 7 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Hasanuddin University
  • Sebelas Maret University
  • Jenderal Soedirman University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Slamet SlametSebelas Maret University4
Aji PrasetyaningrumAffiliation unknown3
Bakti JosAffiliation unknown2
Muhammad IbadurrohmanAffiliation unknown2
Dewi AnggrainiUniversitas Halu Oleo1
Rahman TandiAffiliation unknown1
Yusuf Musafir KoleworaUniversitas Halu Oleo1
Faisal YunusAffiliation unknown1
Surdin SurdinUniversitas Halu Oleo1
La HaruduAffiliation unknown1
Ibnu Amar MuchsinAffiliation unknown1
Mustika MustikaAffiliation unknown1
Maskun MaskunHasanuddin University1
Supardi SalamAffiliation unknown1
Puji LestariJenderal Soedirman University1
Zulfina AdrianiAffiliation unknown1
Randa KrismonAffiliation unknown1
Muazzinah MuazzinahAffiliation unknown1
Zakki Fuad KhalilAffiliation unknown1
Cut ZamhariraAffiliation unknown1
Putri MarzaniarAffiliation unknown1
Lisa Indriani BangkeleAffiliation unknown1
SayaniAffiliation unknown1
MinarniAffiliation unknown1
JumardinAffiliation unknown1
ABDUL RAHMANUniversitas Halu Oleo1
Zainal Amin AyubAffiliation unknown1
Mohammad Zen RahfiludinAffiliation unknown1
Suparmi SuparmiSebelas Maret University1
Veramita Nanda PradanaAffiliation unknown1
Atina HussaanaAffiliation unknown1
Siti Thomas ZulaikhahAffiliation unknown1
Farida NailufarAffiliation unknown1
Endah WahyutriAffiliation unknown1
Riska Mayang Saputri GintingAffiliation unknown1
Fit NawatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.uinsgd.ac.id/index.php/ja/oai, first harvested 7 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Isolasi dan karakterisasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” dan potensinya menghambat penyakit bercak ungu Alternaria porri (ELL) CIF</dc:title>
	<dc:creator>Sudewi, Sri</dc:creator>
	<dc:creator>Ratnawati, Ratnawati</dc:creator>
	<dc:creator>Jaya, Kasman</dc:creator>
	<dc:creator>Hardiyanti, Sitti</dc:creator>
	<dc:subject xml:lang="en-US">Alternaria porri</dc:subject>
	<dc:subject xml:lang="en-US">Antagonisme</dc:subject>
	<dc:subject xml:lang="en-US">Bawang merah</dc:subject>
	<dc:subject xml:lang="en-US">Cendawan endofit</dc:subject>
	<dc:subject xml:lang="en-US">Pengendalian hayati</dc:subject>
	<dc:description xml:lang="en-US">The decrease in shallot production due to Alternaria porri infection can reach 50%-70% or can even cause crop failure. One of the biological control methods to overcome this is by utilizing antagonistic microbes. The purpose of this study was to identify endophytic fungi from the rhizosphere of shallots "Lembah Palu " that have the potential to inhibit the A. porri fungus that causes purple spot disease. Research January-September 2022, the activity began with isolating endophytic fungi using the surface sterilization method, followed by characterization and identification of morphology macroscopically and microscopically, compatibility testing between isolates, and antagonism power test. The results obtained 5 isolates of endophytic fungi identified in the genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, and Trichoderma sp. A total of seven compatible interactions between endophytic fungi resulted from compatibility tests, while 3 other interactions were not compatible. The antagonism test of 5 fungal isolates against the A. porri produced an average inhibition of ≥ 75%. The HKP5 isolate (Penicillium sp) had the best inhibition of 78.96%. This indicates that endophytic fungal isolates have some potencies the potential to be developed as biological control agents in an effort to support sustainable agriculture.ABSTRAKPenurunan produksi tanaman bawang merah akibat serangan Alternaria porri dapat mencapai 50%-70% bahkan dapat menyebabkan terjadinya gagal panen. Salah satu metode pengendalian hayati untuk mengatasi hal tersebut yaitu dengan memanfaatkan mikroba yang bersifat antagonis . Tujuan dari penelitian ini untuk mengidentifikasi cendawan endofit asal rizosfer bawang merah “Lembah Palu” yang berpotensi dalam menghambat cendawan A. porri penyebab penyakit bercak ungu. Penelitian Januari-September 2022, kegiatan diawali dengan mengisolasi cendawan endofit menggunakan metode sterilisasi permukaan, dengan perendaman sampel akar berurutan dalam 2% Natrium hipoklorit, etanol 70% dan air steril, kemudian dikulturkan dalam media Potato Dextrose Agar (PDA). Selanjutnya setelah isolat tumbuh, disubkultur kembali untuk dilakukan karakterisasi dan identifikasi morfologi secara makroskopis dan mikroskopis, pengujian kompatibilitas antarisolat serta uji daya antagonisme. Dari hasil penelitian diperoleh 5 isolat cendawan endofit yang teridentifikasi dalam genus Fusarium sp., Aspergillus flavus, Penicillium sp., Aspergillus niger, dan Trichoderma sp. Sebanyak tujuh interaksi kompatibel antarcendawan endofit dihasilkan dari uji kompatibilitas, sedangkan 3 interaksi lainnya tidak kompatibel. Uji daya antagonisme 5 isolat cendawan terhadap patogen A. porri menghasilkan rata-rata daya hambat ≥ 75%. Isolat HKP5 (Penicillium sp) memiliki daya hambat terbaik sebesar 78,96%. Hal ini mengindikasikan bahwa isolat cendawan endofit berpotensi untuk dikembangkan sebagai agens pengendali hayati dalam upaya mendukung pertanian berkelanjutan.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Agroteknologi</dc:publisher>
	<dc:date>2023-12-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:identifier>https://journal.uinsgd.ac.id/index.php/ja/article/view/28242</dc:identifier>
	<dc:identifier>10.15575/28242</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal AGRO; Vol. 10 No. 2 (2023); 278-292</dc:source>
	<dc:source>2407-7933</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinsgd.ac.id/index.php/ja/article/view/28242/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Sri Sudewi</dc:rights>
</oai_dc:dc>