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Effect of Colchicine Concentrations and Soaking Period on Ploidy of Katokkon Chili (Capsicum chinense Jacq.) at Seedlings Stage

AGRIVITA, Journal of Agricultural Science · Vol. 46 No. 3 (2024) · pp. 506–517

DOI
10.17503/agrivita.v46i3.3936
ISSN
2477-8516, 0126-0537
Published
2024-09-05
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

This research is conducted to study the induction of polyploidization in Katokkon chili using colchicine to study the effect of chromosome doubling or polyploid on certain superior characters during the seedling phase. Some morphological alterations were determined and proven to persist during the seedling phase. The study was conducted in the laboratory and screen house of the Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin. The polyploidy of the Katokkon chili is induced by soaking the germinated seeds in 0%, 0.05%, 0.1%, and 0.2% colchicine solutions for 6, 12, 24, and 48 hours each. Ploidy levels are analyzed using flow cytometry (Partec®Cy-Flow Space TM). Results show significant differences in colchicine treatment affecting all parameters observed: hypocotyl base of sprouts, plant height, stomata size, and the number of lateral shoots. Flow cytometry analysis histogram confirmed that administration of 0.1% colchicine concentration with 48 hours soaking time and 0.2% colchicine concentration with 24- or 48-hour soaking time can induce tetraploid plants (4n=48) with different coefficients of variance (CV-x%), 5.36%, 4.65%, 6.08%, respectively. Vigorous growth phenotype in leaf size and plant height was more clearly seen in the one-month-old tetraploid Katokkon chili seedlings induced by 0.10% with a soaking time of 48 hours.

Keywords

Collaboration network of the authors

40 authors · 246 co-authorship links · 7 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Hasanuddin University
  • Sekolah Tinggi Ilmu Kesehatan Tamalatea Makassar
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Hari IswoyoHasanuddin University16
Katriani MantjaHasanuddin University13
Tigin DariatiHasanuddin University12
Cri Wahyuni Brahmi YantiHasanuddin University10
Rahmansyah DermawanHasanuddin University8
Nurfaida NurfaidaHasanuddin University7
NurfaidaHasanuddin University5
Muhammad Fuad AnshoriHasanuddin University5
Nurhaya J. PangaAffiliation unknown5
Syatrianty Andi SyaifulHasanuddin University4
Feranita HaringHasanuddin University4
Nuniek WidiayaniHasanuddin University4
Rahmansyah DermawanHasanuddin University4
Elkawakib Syam’unHasanuddin University4
A. R. TrisnawatyAffiliation unknown4
Feranita HaringAffiliation unknown4
Astina TambungHasanuddin University3
Rizza Nurul ApriliaHasanuddin University3
Muh. Farid BdrHasanuddin University3
Muhammad FariedHasanuddin University3
Muh. RiadiAffiliation unknown3
Rafiuddin RafiuddinHasanuddin University3
Feranita HaringAffiliation unknown3
Muh RiadiAffiliation unknown3
Novaty Eny DunggaHasanuddin University2
AMIR YASSIHasanuddin University2
Abdul MollahAffiliation unknown2
Hatta JamilHasanuddin University2
. NasaruddinAffiliation unknown2
Sakka PatiHasanuddin University2
Muh FaridHasanuddin University2
Ahmad Fauzan AdzimaHasanuddin University2
Amin NurSekolah Tinggi Ilmu Kesehatan Tamalatea Makassar2
Rusnadi PadjungHasanuddin University2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Colchicine Concentrations and Soaking Period on Ploidy of Katokkon Chili (Capsicum chinense Jacq.) at Seedlings Stage</dc:title>
	<dc:creator>Sjahril, Rinaldi</dc:creator>
	<dc:creator>Riadi, Muhammad</dc:creator>
	<dc:creator>Ridwan, Ifayanti</dc:creator>
	<dc:creator>Kasim, Nurlina</dc:creator>
	<dc:creator>Tambung, Astina</dc:creator>
	<dc:creator>Novitasari, Novitasari</dc:creator>
	<dc:subject xml:lang="en-US">Agronomy</dc:subject>
	<dc:subject xml:lang="en-US">Colchicine; Flow cytometry analysis; Katokkon chili; Polyploidization; Tetraploid</dc:subject>
	<dc:description xml:lang="en-US">This research is conducted to study the induction of polyploidization in Katokkon chili using colchicine to study the effect of chromosome doubling or polyploid on certain superior characters during the seedling phase. Some morphological alterations were determined and proven to persist during the seedling phase. The study was conducted in the laboratory and screen house of the Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin. The polyploidy of the Katokkon chili is induced by soaking the germinated seeds in 0%, 0.05%, 0.1%, and 0.2% colchicine solutions for 6, 12, 24, and 48 hours each. Ploidy levels are analyzed using flow cytometry (Partec®Cy-Flow Space TM). Results show significant differences in colchicine treatment affecting all parameters observed: hypocotyl base of sprouts, plant height, stomata size, and the number of lateral shoots. Flow cytometry analysis histogram confirmed that administration of 0.1% colchicine concentration with 48 hours soaking time and 0.2% colchicine concentration with 24- or 48-hour soaking time can induce tetraploid plants (4n=48) with different coefficients of variance (CV-x%), 5.36%, 4.65%, 6.08%, respectively. Vigorous growth phenotype in leaf size and plant height was more clearly seen in the one-month-old tetraploid Katokkon chili seedlings induced by 0.10% with a soaking time of 48 hours.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">The Ministry of Education, Culture, Research and Technology of Indonesia</dc:contributor>
	<dc:date>2024-09-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3936</dc:identifier>
	<dc:identifier>10.17503/agrivita.v46i3.3936</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 46, No 3 (2024); 506-517</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v46i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3936/1786</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/2711</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/3256</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3936/3257</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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