Melola Discovery

← Melola Discovery

Open access Journal SINTA 1

Segregation Patterns and Proportion of Pod Color in Purple Winged Bean (Psophocarpus tetragonolobus L.) F3 and F4 Populations

AGRIVITA, Journal of Agricultural Science · Vol. 48 No. 3 (2026) · pp. 693–702

DOI
10.17503/agrivita.v48i3.4997
ISSN
2477-8516, 0126-0537
Published
2026-09-30
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Winged bean (Psophocarpus tetragonolobus L.), an underutilized tropical legume, is valued for its nutrient content and anthocyanin-rich purple pods. Genetic improvement is critically needed for superior varieties, yet a fundamental understanding of target trait inheritance, especially pod color in advanced generations, remains limited. This study investigated pod color segregation patterns, gene number, and gene action in F3 and F4 winged bean populations. Using 46 F3 and 79 F4 individuals from a green-podded (PLB 2.3) x purple-podded (MDM 1.2) cross, qualitative data were analyzed using Mendelian ratios and chi-square tests. Results consistently showed a 13 green: 3 purple pod color segregation ratio in both F3 and F4 populations, indicating control by two gene pairs with dominant-recessive epistasis. Chi-square tests confirmed good fit for this model in F3 (X2 =0.06, p=0.8) and F4 (X2=0.40, p=0.5−0.7). This robust pattern confirms inheritance stability; the purple phenotype (aaB_) is expressed only when the inhibitory gene is homozygous recessive ('aa') and the color-forming gene is dominant ('B_'). These findings provide crucial genetic insights, facilitating targeted breeding for superior purple-podded winged bean varieties, and suggest F2 analysis might not be strictly necessary for initial genetic inference of this trait due to the observed consistency in advanced generations.

Keywords

Collaboration network of the authors

40 authors · 104 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Walailak University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Budi WaluyoWalailak University5
Darmawan SaptadiAffiliation unknown3
Sumeru AshariAffiliation unknown2
Niken KendariniAffiliation unknown2
Prakit SomtaAffiliation unknown2
Irzal AndersonAffiliation unknown2
Arifin Noor SugihartoAffiliation unknown2
Devi MienantiAffiliation unknown2
Iqbal HidayatAffiliation unknown2
Yanuar DanaatmadjaAffiliation unknown2
Mohammad Bahrelfi BelaffifAffiliation unknown2
Ajay Goel KumarAffiliation unknown2
Mukhammad KundoriAffiliation unknown1
Muhammad Arif LiputoAffiliation unknown1
Nina ArdiniAffiliation unknown1
Aisyah Lukitatary PramessariardaAffiliation unknown1
Refnida RefnidaAffiliation unknown1
Tatik WardiyatiAffiliation unknown1
Nur AzizahAffiliation unknown1
Aprilia RidhawatiAffiliation unknown1
Abdul Latief AbadiAffiliation unknown1
Ardiyan TarunaAffiliation unknown1
Amalia Khairunnisa’Affiliation unknown1
Rifani Rusiana DewiAffiliation unknown1
Erekso HadiwijoyoAffiliation unknown1
Muhammad Akhid Syib’liAffiliation unknown1
Hendrawan SoetantoAffiliation unknown1
Markus Miten KledenAffiliation unknown1
Kusmartono KusmartonoAffiliation unknown1
I. YulianahAffiliation unknown1
D. SaptadiAffiliation unknown1
R. D. AndayaniAffiliation unknown1
Karuniawan Puji WicaksonoAffiliation unknown1
Edson BegliominiAffiliation unknown1
Sudakir SudakirAffiliation unknown1
Catur Suciari KurniaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Segregation Patterns and Proportion of Pod Color in Purple Winged Bean (Psophocarpus tetragonolobus L.) F3 and F4 Populations</dc:title>
	<dc:creator>Mahdy, Ahmad Alif Riyan</dc:creator>
	<dc:creator>Yulianah, Izmi</dc:creator>
	<dc:creator>Rahma, Rima</dc:creator>
	<dc:creator>Kuswanto, Kuswanto</dc:creator>
	<dc:subject xml:lang="en-US">agronomy; horticulture; plant breeding</dc:subject>
	<dc:subject xml:lang="en-US">Dominant recessive epistasis, Gene action, Inheritance , Pod color, Winged bean</dc:subject>
	<dc:description xml:lang="en-US">Winged bean (Psophocarpus tetragonolobus L.), an underutilized tropical legume, is valued for its nutrient content and anthocyanin-rich purple pods. Genetic improvement is critically needed for superior varieties, yet a fundamental understanding of target trait inheritance, especially pod color in advanced generations, remains limited. This study investigated pod color segregation patterns, gene number, and gene action in F3 and F4 winged bean populations. Using 46 F3 and 79 F4 individuals from a green-podded (PLB 2.3) x purple-podded (MDM 1.2) cross, qualitative data were analyzed using Mendelian ratios and chi-square tests. Results consistently showed a 13 green: 3 purple pod color segregation ratio in both F3 and F4 populations, indicating control by two gene pairs with dominant-recessive epistasis. Chi-square tests confirmed good fit for this model in F3 (X2 =0.06, p=0.8) and F4 (X2=0.40, p=0.5−0.7). This robust pattern confirms inheritance stability; the purple phenotype (aaB_) is expressed only when the inhibitory gene is homozygous recessive ('aa') and the color-forming gene is dominant ('B_'). These findings provide crucial genetic insights, facilitating targeted breeding for superior purple-podded winged bean varieties, and suggest F2 analysis might not be strictly necessary for initial genetic inference of this trait due to the observed consistency in advanced generations.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US">Ministry of Education, Culture, Research, and Technology (KEMENDIKBUDRISTEK) for funding this research under the BIMA Master's Thesis Program 2024.</dc:contributor>
	<dc:date>2026-09-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4997</dc:identifier>
	<dc:identifier>10.17503/agrivita.v48i3.4997</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 48, No 3 (2026): October 2026; 693-702</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v48i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4997/2062</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2026 Ahmad Alif Riyan Mahdy</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>