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Nitrogenase Activity and IAA Production of Indigenous Diazotroph and Its Effect on Rice Seedling Growth

AGRIVITA, Journal of Agricultural Science · Vol. 39 No. 1 (2016) · pp. 31–37

DOI
10.17503/agrivita.v39i1.653
ISSN
2477-8516, 0126-0537
Published
2016-12-19
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0/

Abstract

The diazotroph bacteria as ecofriendly biofer-tilizers play an important role in improving the N status and availability of paddy soil. Laboratory experiment to study nitrogenase activity and IAA production of diazotroph from rice rhizosphere and to assess its effect on the growth of rice seedling has been conducted from September to October 2014 in Agronomy and Horticulture Laboratory Faculty of Agriculture Jenderal Soedirman University, Purwokerto. The experiment was arranged as a completely randomized design and consisted of seven treatments and provided with 4 replications. The treatments were the isolates of indegenous diazotroph (T1 = A11003, T2 = A230041, T3 = A24001, T4 = A230022, T5 = A230021, T6 = A230042 and T7 = without inoculation). The nitrogenase activity measured by acetylene reduction assay method and IAA production was measured by HPLC method. Plant height, leaf greeness, leaf area, total dry weight and total root length were determined on 21 days after sowing. The experimental results showed that the isolat A230021 was identified as Rhizobium sp. LM-5 and have the highest nitrogenase activity at 0.07 µM C2H4 ml-1 h-1 and IAA production reached 19.01 ppm. Inoculation with strains of diazotroph enhanced chlorophyl content, total root length, and biomass production.

Keywords

Collaboration network of the authors

40 authors · 84 co-authorship links · 12 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Cenderawasih University
  • Hasanuddin University
  • State University of Malang
  • Universitas Gajayana Malang
  • Universitas Muhammadiyah Metro
  • Universiti Utara Malaysia
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Mieke Rochimi SetiawatiAffiliation unknown5
Gunadi GunadiUniversitas Gajayana Malang4
Widyatmani Sih DewiAffiliation unknown4
Supriyadi SupriyadiAffiliation unknown4
Sri HartatiAffiliation unknown4
Dedi RuswandiAffiliation unknown3
Sudadi SudadiAffiliation unknown3
Istadi IstadiAffiliation unknown3
Luqman BuchoriAffiliation unknown3
Retno RosariastutiAffiliation unknown3
Anne NurainiAffiliation unknown3
Muhammad Isran RamliHasanuddin University2
Ibnu SiswantoAffiliation unknown2
Ni Wayan Anik LeanaAffiliation unknown2
Eka OktavianiAffiliation unknown2
Triyanto TriyantoAffiliation unknown2
Yenny Aman SerahAffiliation unknown2
Resmaya Agnesia Mutiara SiraitAffiliation unknown2
Agustinus AstonoAffiliation unknown2
Fakhri Nasharul SyihabAffiliation unknown2
Nadia Nuraniya KamaluddinAffiliation unknown2
Betty Natalie FitriatinAffiliation unknown2
Ongko CahyonoAffiliation unknown2
Budiyono BudiyonoAffiliation unknown2
Sumani SumaniAffiliation unknown2
Denny Nugroho SugiantoAffiliation unknown2
Muliyadi MuliyadiAffiliation unknown2
Sri SumiyatiAffiliation unknown2
Rosye H.R. TanjungCenderawasih University1
Suriani Br. SurbaktiCenderawasih University1
Dian Dwi Apriliyani ArsdinHasanuddin University1
Nuzul HidayatAffiliation unknown1
SUDIRMAN SUDIRMANState University of Malang1
Nego LinuhungUniversitas Muhammadiyah Metro1
Sukoriyanto SukoriyantoState University of Malang1
Mohd Isha AwangUniversiti Utara Malaysia1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Nitrogenase Activity and IAA Production of Indigenous Diazotroph and Its Effect on Rice Seedling Growth</dc:title>
	<dc:creator>Purwanto, Purwanto</dc:creator>
	<dc:creator>Yuwariah, Yuyun</dc:creator>
	<dc:creator>Sumadi, Sumadi</dc:creator>
	<dc:creator>Simarmata, Tualar</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">diazotroph; IAA; N fixation; nitrogenase; plant growth</dc:subject>
	<dc:description xml:lang="en-US">The diazotroph bacteria as ecofriendly biofer-tilizers play an important role in improving the N status and availability of paddy soil. Laboratory experiment to study nitrogenase activity and IAA production of diazotroph from rice rhizosphere and to assess its effect on the growth of rice seedling has been conducted from September to October 2014 in Agronomy and Horticulture Laboratory Faculty of Agriculture Jenderal Soedirman University, Purwokerto. The experiment was arranged as a completely randomized design and consisted of seven treatments and provided with 4 replications. The treatments were the isolates of indegenous diazotroph (T1 = A11003, T2 = A230041, T3 = A24001, T4 = A230022, T5 = A230021, T6 = A230042 and T7 = without inoculation). The nitrogenase activity measured by acetylene reduction assay method and IAA production was measured by HPLC method. Plant height, leaf greeness, leaf area, total dry weight and total root length were determined on 21 days after sowing. The experimental results showed that the isolat A230021 was identified as Rhizobium sp. LM-5 and have the highest nitrogenase activity at 0.07 µM C2H4 ml-1 h-1 and IAA production reached 19.01 ppm. Inoculation with strains of diazotroph enhanced chlorophyl content, total root length, and biomass production.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
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	<dc:date>2016-12-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/653</dc:identifier>
	<dc:identifier>10.17503/agrivita.v39i1.653</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 39, No 1 (2017): FEBRUARY; 31-37</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v39i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/653/831</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 AGRIVITA Journal of Agricultural Science</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
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