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IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE

AGRIVITA, Journal of Agricultural Science · Vol. 37 No. 1 (2015) · pp. 51–58

DOI
10.17503/agrivita.v37i1.438
ISSN
2477-8516, 0126-0537
Published
2015-02-05
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0/

Abstract

The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets. Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals. Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.

Keywords

Collaboration network of the authors

40 authors · 107 co-authorship links · 4 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • State University of Jakarta
  • Yarsi University
  • University of Indonesia
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agustiansyah AgustiansyahAffiliation unknown4
Agus KaryantoAffiliation unknown4
Joko JokoUniversitas Halu Oleo2
Adi Noor PrayogiAffiliation unknown2
Wiry PungkastianiAffiliation unknown2
Setyo Dwi UtomoAffiliation unknown2
Hayane Adeline WarganegaraAffiliation unknown2
Nanik SriyaniAffiliation unknown2
Desi MaulidaAffiliation unknown2
Rahmadyah HamirantiAffiliation unknown2
La Ode Herman HalikaAffiliation unknown1
Novi NoviAffiliation unknown1
La IbaUniversitas Halu Oleo1
Fitriani FitrianiAffiliation unknown1
Adrian TawaiUniversitas Halu Oleo1
Silviatun NihayahAffiliation unknown1
Wening SariAffiliation unknown1
Intan RazariAffiliation unknown1
Kinasih PrayuniAffiliation unknown1
Ahmad Rusdan Handoyo UtomoAffiliation unknown1
Sudarsono SudarsonoAffiliation unknown1
Widodo WidodoState University of Jakarta1
. YUSNITAAffiliation unknown1
Muhammad HidayatAffiliation unknown1
Fathul JannahAffiliation unknown1
Tri Edhi Budhi SoesiloUniversity of Indonesia1
Kholis ErnawatiYarsi University1
Qatrunnada NadhifahAffiliation unknown1
Anis MuslikhaAffiliation unknown1
Dini WidiantiAffiliation unknown1
Sudarsono .Affiliation unknown1
JumantoAffiliation unknown1
Hajrial AswidinnoorAffiliation unknown1
RUSMILAH SUSENOAffiliation unknown1
CIPTA GINTINGAffiliation unknown1
Suskandini Ratih DirmawatiAffiliation unknown1
Ivayani IvayaniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE</dc:title>
	<dc:creator>Yusnita, Yusnita</dc:creator>
	<dc:creator>Danial, Ekawati</dc:creator>
	<dc:creator>Hapsoro, Dwi</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">acclimatization; benzyladenine; fertilizer; genotypes;  media mixtures</dc:subject>
	<dc:description xml:lang="en-US">The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets.  Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals.  Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-02-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/438</dc:identifier>
	<dc:identifier>10.17503/agrivita.v37i1.438</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 37, No 1 (2015): FEBRUARY; 51-58</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v37i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/438/472</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 AGRIVITA Journal of Agricultural Science</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
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