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Molecular Identification of Microbes from the Soil Rhizosphere of Cocoa as A Potential Biofertilizer

AGRIVITA, Journal of Agricultural Science · Vol. 45 No. 1 (2023) · pp. 124–130

DOI
10.17503/agrivita.v45i1.3840
ISSN
2477-8516, 0126-0537
Published
2023-02-27
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

There are three microbes that originated from the roots (Rhizosphere) of the cocoa plant (Theobroma cacao L.) as a potential biofertilizer. This research aimed to specify the ability of microbial isolates in a consortium with the addition of Biosurfactant Diethanolamide. The three microbes were observed using synergism test, molecular and population identification in the consortium. The synergism test showed the three isolates were not mutually antagonistic indicated there was no clear zone around the colony. The molecular identification showed the isolate 1 was as Bacillus substilis (99.88% hegemony), isolate 2 was as Bacillus substilis (99.75% hegemony) and isolate 3 was as Bacillus amyloliquefaciens (100% hegemony). The population consisted of 3.6 x 106 cfu/ml Azotobacter, 0.55 x 101 cfu/ml Azospirillium and 2.5 x 105 cfu/ml phosphate solubilizing bacteria. The results of the calculations on the microbial consortium with the addition of Biosurfactant Diethanolamide were 3.75 x 106 cfu/ml Azotobacter, 0.1 x 102 cfu/ml Azospirillium and 1.65 x 105 cfu/ml phosphate solubilizing bacteria.

Keywords

Collaboration network of the authors

40 authors · 97 co-authorship links · 1 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Sultan Ageng Tirtayasa University
  • Universitas Halu Oleo
  • Hasanuddin University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Dewi FirniaSultan Ageng Tirtayasa University2
Putra UtamaSultan Ageng Tirtayasa University2
Endang SulistyoriniSultan Ageng Tirtayasa University2
Adha Mashur SajiahUniversitas Halu Oleo1
Ika Purwanti NingrumUniversitas Halu Oleo1
Jayanti Yusmah SariUniversitas Halu Oleo1
Natalis RansiUniversitas Halu Oleo1
Ayu Sabrina SyairUniversitas Halu Oleo1
Yuwanda PurnamasariAffiliation unknown1
Asih SetiyoriniAffiliation unknown1
WD. Rizqanun Karyima BoluAffiliation unknown1
Novi SaskiaSultan Ageng Tirtayasa University1
Firda RamadiniSultan Ageng Tirtayasa University1
Andree SaylendraSultan Ageng Tirtayasa University1
Sri Astria Amanda SyifaSultan Ageng Tirtayasa University1
Deannisa NirmalaSultan Ageng Tirtayasa University1
Susiyanti SusiyantiSultan Ageng Tirtayasa University1
Vega Yoesepa PamelaAffiliation unknown1
Septariawulan KusumasariAffiliation unknown1
Muhammad Rizal FebriansahAffiliation unknown1
Rifqi Ahmad RiyantoAffiliation unknown1
Athifa Lisna WidyatiktaAffiliation unknown1
Vega Yoesepa PamelaAffiliation unknown1
Rifqi Ahmad RiyantoAffiliation unknown1
Kartina KartinaHasanuddin University1
Susiyanti SusiyantiAffiliation unknown1
A. CitraresminiAffiliation unknown1
I. AnasAffiliation unknown1
I Made SudianaAffiliation unknown1
Sri WidawatiAffiliation unknown1
Suliasih SuliasihAffiliation unknown1
Yuki MuramatsuAffiliation unknown1
TEDJA -IMASAffiliation unknown1
ALFI DATIN ZAUQIAHAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Molecular Identification of Microbes from the Soil Rhizosphere of Cocoa as A Potential Biofertilizer</dc:title>
	<dc:creator>Nurmayulis, Nurmayulis</dc:creator>
	<dc:creator>Sodiq, Abdul Hasyim</dc:creator>
	<dc:creator>Eris, Fitria Riany</dc:creator>
	<dc:creator>Hastuti, Dewi</dc:creator>
	<dc:creator>Denny, Yus Rama</dc:creator>
	<dc:creator>Susilowati, Dwi Ningsih</dc:creator>
	<dc:subject xml:lang="en-US">soil-plant sciences</dc:subject>
	<dc:subject xml:lang="en-US">Bacillus substilis; Bacillus amyloliquefaciens; Biosurfactant; Biofertilizer</dc:subject>
	<dc:description xml:lang="en-US">There are three microbes that originated from the roots (Rhizosphere) of the cocoa plant (Theobroma cacao L.) as a potential biofertilizer. This research aimed to specify the ability of microbial isolates in a consortium with the addition of Biosurfactant Diethanolamide. The three microbes were observed using synergism test, molecular and population identification in the consortium. The synergism test showed the three isolates were not mutually antagonistic indicated there was no clear zone around the colony. The molecular identification showed the isolate 1 was as Bacillus substilis (99.88% hegemony), isolate 2 was as Bacillus substilis (99.75% hegemony) and isolate 3 was as Bacillus amyloliquefaciens (100% hegemony). The population consisted of  3.6 x 106 cfu/ml Azotobacter, 0.55 x 101 cfu/ml Azospirillium and 2.5 x 105 cfu/ml phosphate solubilizing bacteria. The results of the calculations on the microbial consortium with the addition of Biosurfactant Diethanolamide were 3.75 x 106 cfu/ml Azotobacter, 0.1 x 102 cfu/ml Azospirillium and 1.65 x 105 cfu/ml phosphate solubilizing bacteria.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2023-02-27</dc:date>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3840</dc:identifier>
	<dc:identifier>10.17503/agrivita.v45i1.3840</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 45, No 1 (2023); 124-130</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v45i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/3840/1538</dc:relation>
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	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3840/2575</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3840/2576</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/3840/2601</dc:relation>
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	<dc:rights xml:lang="en-US">Copyright (c) 2023 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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