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Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Atom Indonesia · Vol. 43 No. 2 (2017) · pp. 103–109

DOI
10.17146/aij.2017.685
ISSN
2356-5322, 0126-1568
Published
2017-08-23
Publisher
National Research and Innovation Agency
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

The objectives of this research wereto evaluate (1) the performance of soybean mutant lines under drought stress conditions, and(2) the genetic diversity and relationship among the mutant lines using SSR markers.The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR) loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8,showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3and Kdl8, which yielded 1.75 t ha-1and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant linesrequired 30.75 to 32days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cmrespectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namelygroup I (the Panderman with three mutant lines) and group II (the Muria with two mutant lines). Twenty-three out of 40 evaluated SSR loci, including AW31, BE806, CMAC7L, S080, S126, S57, S171, S224, S285, S294, S393, S294, S383, S511, S511, S520, S540, S547, S551, S571, S577, and S578, provided polymorphic alleles between the parents and their mutants and could be used to differentiate mutants from their parents. Therefore, these loci are informative and may be useful for further analysis of soybean mutant lines in relation to breeding program.

Keywords

Collaboration network of the authors

25 authors · 78 co-authorship links · 24 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • IPB University
  • Sebelas Maret University
  • National Nuclear Energy Agency of Indonesia
  • National Research and Innovation Agency
  • University of Indonesia
  • Indonesian National Institute of Aeronautics and Space
  • Other organisations
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Media Fitri Isma NugrahaAffiliation unknown2
Hessy NovitaNational Research and Innovation Agency2
Rossa YunitaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Wening EnggariniIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development2
Muh. Alias L. RajamuddinIPB University1
Fasya Hadaina MaharaniAffiliation unknown1
Berna ElyaUniversity of Indonesia1
Atriyon JulzarikaIndonesian National Institute of Aeronautics and Space1
Alias RadjamuddinAffiliation unknown1
Nurmalita WaluyoAffiliation unknown1
Iteu Margaret HidayatAffiliation unknown1
Tri HandayaniIndonesian Institute of Sciences1
Ineu SulastriniAffiliation unknown1
Salamiah SalamiahAffiliation unknown1
Lelya PramudyaniAffiliation unknown1
Sudarsono SudarsonoIPB University1
Hajrial AswidinnoorAffiliation unknown1
Dwinita Wikan UtamiAffiliation unknown1
SUGIONO MOELJOPAWIROAffiliation unknown1
IDA HANARIDAAffiliation unknown1
Edi PurwantoSebelas Maret University1
Nina Agusti WidaningsihIPB University1
N. NandariyahSebelas Maret University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://atomindonesia.brin.go.id/index.php/aij/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers</dc:title>
	<dc:creator>Yuliasti, Y</dc:creator>
	<dc:creator>Reflinur, R</dc:creator>
	<dc:subject xml:lang="en-US">Supply some 4–6 keywords; Soybean; Mutant; Drought tolerance; SSR markers analysis</dc:subject>
	<dc:description xml:lang="en-US">The objectives of this research wereto evaluate (1) the performance of soybean mutant lines under drought stress conditions, and(2) the genetic diversity and relationship among the mutant lines using SSR markers.The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR) loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8,showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3and Kdl8, which yielded 1.75 t ha-1and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant linesrequired 30.75 to 32days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cmrespectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namelygroup I (the Panderman with three mutant lines) and group II (the Muria with two mutant lines). Twenty-three out of 40 evaluated SSR loci, including AW31, BE806, CMAC7L, S080, S126, S57, S171, S224, S285, S294, S393, S294, S383, S511, S511, S520, S540, S547, S551, S571, S577, and S578, provided polymorphic alleles between the parents and their mutants and could be used to differentiate mutants from their parents. Therefore, these loci are  informative and may be useful for further analysis of soybean mutant lines in relation to breeding program.</dc:description>
	<dc:publisher xml:lang="en-US">National Research and Innovation Agency</dc:publisher>
	<dc:contributor xml:lang="en-US">National Nuclear Energy Agency</dc:contributor>
	<dc:date>2017-08-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://atomindonesia.brin.go.id/index.php/aij/article/view/685</dc:identifier>
	<dc:identifier>10.17146/aij.2017.685</dc:identifier>
	<dc:source xml:lang="en-US">Atom Indonesia; Vol 43, No 2 (2017): August 2017; 103-109</dc:source>
	<dc:source>2356-5322</dc:source>
	<dc:source>0126-1568</dc:source>
	<dc:source>10.55981/aij.v43i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://atomindonesia.brin.go.id/index.php/aij/article/view/685/512</dc:relation>
	<dc:relation>https://atomindonesia.brin.go.id/index.php/aij/article/downloadSuppFile/685/689</dc:relation>
	<dc:relation>https://atomindonesia.brin.go.id/index.php/aij/article/downloadSuppFile/685/681</dc:relation>
	<dc:relation>https://atomindonesia.brin.go.id/index.php/aij/article/downloadSuppFile/685/682</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Atom Indonesia</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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