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BINARY VECTOR CONSTRUCTION AND Agrobacterium tumefaciens-MEDIATED TRANSFORMATION OF LYSOZYME GENE IN SEAWEED Kappaphycus alvarezii

BIOTROPIA · Vol. 21 No. 2 (2014) · pp. 82–92

DOI
10.11598/btb.2014.21.2.348
ISSN
1907-770X, 0215-6334
Published
2014-11-28
Publisher
SEAMEO BIOTROP
Language
en
License
creativecommons.org/licenses/by-nc-nd/4.0

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.

Collaboration network of the authors

40 authors · 99 co-authorship links · 7 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Sulawesi Tenggara
  • State University of Semarang
  • Hasanuddin University
  • Diponegoro University
  • Universitas Sulawesi Barat
  • Universitas Airlangga
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Sri KhayatiUniversitas Sulawesi Tenggara2
Edy CahyonoState University of Semarang2
Dante AlighiriState University of Semarang2
Nugrahaningsih WHAffiliation unknown2
Adrian Maulana ZakyAffiliation unknown2
Sabila AisyahAffiliation unknown2
Bella PermatasariAffiliation unknown2
Suharsono SuharsonoAffiliation unknown2
Nur Bambang Priyo UtomoAffiliation unknown2
Sri NuryatiAffiliation unknown2
Nampiah SukarnoAffiliation unknown2
LATIFAH KOSIM DARUSMANAffiliation unknown2
ROHANI CINTA BADIA GINTINGAffiliation unknown2
SIHEGIKO KANAYAAffiliation unknown2
AlimuddinAffiliation unknown2
Djohar ArifinUniversitas Sulawesi Tenggara1
Rifkah Anniza RahmanUniversitas Sulawesi Tenggara1
Sri MulyaniDiponegoro University1
Dewi AnggrainiUniversitas Halu Oleo1
Sulsalman MoitaUniversitas Halu Oleo1
Muhammad ArsyadUniversitas Halu Oleo1
Jamaluddin HosUniversitas Halu Oleo1
Wilma J.C. MokaAffiliation unknown1
Nita RukminasariHasanuddin University1
Andi Aliah HidayaniHasanuddin University1
Nur Indah Sari ArbitUniversitas Sulawesi Barat1
Sapto AndriyonoUniversitas Airlangga1
Muhammad NadjibAffiliation unknown1
Saffanah Binti Mohd Ab AzidAffiliation unknown1
Nanik WijayatiState University of Semarang1
Harjono HarjonoAffiliation unknown1
Tjetjep Rohendi RohidiAffiliation unknown1
Fitriani Laili NurAffiliation unknown1
Rizhal Hendi RistantoAffiliation unknown1
Bintang Hanggoro PutraAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">BINARY VECTOR CONSTRUCTION AND Agrobacterium tumefaciens-MEDIATED TRANSFORMATION OF LYSOZYME GENE IN SEAWEED Kappaphycus alvarezii</dc:title>
	<dc:creator xml:lang="en">handayani, Tri</dc:creator>
	<dc:creator xml:lang="en">Alimuddin, Alimuddin</dc:creator>
	<dc:creator xml:lang="en">Widyastuti, Utut</dc:creator>
	<dc:creator xml:lang="en">Suryati, Emma</dc:creator>
	<dc:creator xml:lang="en">Parenrengi, Andi</dc:creator>
	<dc:description xml:lang="en">Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2014-11-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/348</dc:identifier>
	<dc:identifier>10.11598/btb.2014.21.2.348</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014; 82-92</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2014.21.2.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/348/244</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
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