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MOLECULAR CLONING OF THE VITELLOGENIN GENE IN THE HARD-LIPPED BARB (Osteochillus hasseltii C.V.) AND PHOTOPERIOD’S EFFECTS ON GENE EXPRESSION

BIOTROPIA · Vol. 25 No. 3 (2018) · pp. 224–232

DOI
10.11598/btb.2018.25.3.876
ISSN
1907-770X, 0215-6334
Published
2018-09-26
Publisher
SEAMEO BIOTROP
Language
en
License
creativecommons.org/licenses/by-nc-nd/4.0

Abstract

Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P<0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.

Keywords

Collaboration network of the authors

26 authors · 90 co-authorship links

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  • Hasanuddin University
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Endang HilmiAffiliation unknown4
Taufan HarisamAffiliation unknown4
Taufik Budi PramonoAffiliation unknown3
Masashi KawaichiAffiliation unknown3
Arif MahdianaAffiliation unknown2
Teuku JunaidiAffiliation unknown2
Rose DewiAffiliation unknown2
Noprie YansahAffiliation unknown2
Tjahyo WinantoAffiliation unknown2
Sri MarnaniAffiliation unknown2
Agung SudaryonoAffiliation unknown2
Amron AmronAffiliation unknown2
Florencius Eko Dwi HaryonoAffiliation unknown2
Bintang MarhaeniAffiliation unknown2
Eko Setio WibowoAffiliation unknown2
Edy YuwonoAffiliation unknown2
Agus NuryantoAffiliation unknown2
Rita Eka RositaAffiliation unknown2
Hamdan SyakuriAffiliation unknown2
Tri Nur CahyoAffiliation unknown1
Anjalli Tasha ArdiyantoAffiliation unknown1
Sri RahayuHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">MOLECULAR CLONING OF THE VITELLOGENIN GENE IN THE HARD-LIPPED BARB (Osteochillus hasseltii C.V.) AND PHOTOPERIOD’S EFFECTS ON GENE EXPRESSION</dc:title>
	<dc:creator xml:lang="en">Prayogo, Norman Arie</dc:creator>
	<dc:creator xml:lang="en">Siregar, Asrul Sahri</dc:creator>
	<dc:creator xml:lang="en">Sukardi, Purnama</dc:creator>
	<dc:creator xml:lang="en">Bessho, Yasumasa</dc:creator>
	<dc:subject xml:lang="en">amino acid</dc:subject>
	<dc:subject xml:lang="en">cDNA</dc:subject>
	<dc:subject xml:lang="en">Hard-lipped barb</dc:subject>
	<dc:subject xml:lang="en">photoperiods</dc:subject>
	<dc:subject xml:lang="en">vitellogenin</dc:subject>
	<dc:description xml:lang="en">Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P&amp;lt;0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2018-09-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/876</dc:identifier>
	<dc:identifier>10.11598/btb.2018.25.3.876</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018; 224 - 232</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2018.25.3.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/876/424</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>