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EFFECTS OF PHOTOPERIODS ON THE CLONED GtH GENES IN HARD-LIPPED BARB (Osteochilus hasseltii)

BIOTROPIA · Vol. 26 No. 3 (2019) · pp. 191–200

DOI
10.11598/btb.2019.26.3.1100
ISSN
1907-770X, 0215-6334
Published
2019-12-05
Publisher
SEAMEO BIOTROP
Language
en
License
creativecommons.org/licenses/by-nc-nd/4.0

Abstract

Photoperiod is one of the factors that regulate endocrine gland hormone production necessary for gonadal growth and development, gametogenesis, and reproductive cycles in fish. However, studies on the influence of photoperiod on tropical fishes are still limited. Therefore, this study investigated the effects of photoperiod on the hard-lipped barb, particularly the gene expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II). The experiment was designed using three treatments: 14L:10D (control), 8L:16D (short photoperiod), and 18L:6D (long photoperiod). Four aquariums with nine fish per tank were used as replicates. The fish were maintained under these photoperiod treatments for eight weeks. Pituitary activity was observed by measuring GtH gene expression. The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursor encoded by the cDNA consisted of 67 amino acids, including the mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were applied. The results indicated that photoperiod length affects the reproductive performance of the hard-lipped barb.

Keywords

Collaboration network of the authors

40 authors · 117 co-authorship links · showing the 40 most connected

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  • Universitas Gadjah Mada
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Endang HilmiAffiliation unknown4
Taufan HarisamAffiliation unknown4
Yasumasa BesshoAffiliation unknown3
M. MurwantokoAffiliation unknown2
Edy MeiyantoAffiliation unknown2
Muhammad Da’iAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Arif MahdianaAffiliation unknown2
Teuku JunaidiAffiliation unknown2
Rose DewiAffiliation unknown2
Noprie YansahAffiliation unknown2
Tjahyo WinantoAffiliation unknown2
Sri MarnaniAffiliation unknown2
Agung SudaryonoAffiliation unknown2
Amron AmronAffiliation unknown2
Florencius Eko Dwi HaryonoAffiliation unknown2
Bintang MarhaeniAffiliation unknown2
Eko Setio WibowoAffiliation unknown2
Edy YuwonoAffiliation unknown2
Chio OkaAffiliation unknown1
Umar Anggara JenieAffiliation unknown1
Supardjan AMAffiliation unknown1
Barinta WidaryantiAffiliation unknown1
Rarastoeti PratiwiAffiliation unknown1
Dewi Nur'aeni SetyowatiAffiliation unknown1
Widya AsmaraUniversitas Gadjah Mada1
Sri DarmawatiAffiliation unknown1
Wayan T. ArtamaAffiliation unknown1
Nunuk Aries NurulitaAffiliation unknown1
Eishou MatsudaAffiliation unknown1
Hanum Mukti RahayuAffiliation unknown1
Yekti Asih PurwestriAffiliation unknown1
Wahyu Aristyaning PutriAffiliation unknown1
Anis Uswatun KhasanahAffiliation unknown1
Anjalli Tasha ArdiyantoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">EFFECTS OF PHOTOPERIODS ON THE CLONED GtH GENES IN HARD-LIPPED BARB (Osteochilus hasseltii)</dc:title>
	<dc:creator xml:lang="en">Prayogo, Norman Arie</dc:creator>
	<dc:creator xml:lang="en">Pramono, Taufik Budi</dc:creator>
	<dc:creator xml:lang="en">Siregar, Asrul Sahri</dc:creator>
	<dc:creator xml:lang="en">Sukardi, Purnama</dc:creator>
	<dc:creator xml:lang="en">Kawaichi, Masashi</dc:creator>
	<dc:subject xml:lang="en">amino acid</dc:subject>
	<dc:subject xml:lang="en">cDNA</dc:subject>
	<dc:subject xml:lang="en">GtH-I</dc:subject>
	<dc:subject xml:lang="en">GtH-II</dc:subject>
	<dc:subject xml:lang="en">hard lipped barb</dc:subject>
	<dc:subject xml:lang="en">photoperiods</dc:subject>
	<dc:description xml:lang="en">Photoperiod is one of the factors that regulate endocrine gland hormone production necessary for gonadal growth and development, gametogenesis, and reproductive cycles in fish. However, studies on the influence of photoperiod on tropical fishes are still limited. Therefore, this study investigated the effects of photoperiod on the hard-lipped barb, particularly the gene expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II). The experiment was designed using three treatments: 14L:10D (control), 8L:16D (short photoperiod), and 18L:6D (long photoperiod). Four aquariums with nine fish per tank were used as replicates. The fish were maintained under these photoperiod treatments for eight weeks. Pituitary activity was observed by measuring GtH gene expression. The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursor encoded by the cDNA consisted of 67 amino acids, including the mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were applied. The results indicated that photoperiod length affects the reproductive performance of the hard-lipped barb.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2019-12-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1100</dc:identifier>
	<dc:identifier>10.11598/btb.2019.26.3.1100</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019; 191 - 200</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2019.26.3.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1100/556</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>