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GENETIC IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA

BIOTROPIA · Vol. 27 No. 1 (2019) · pp. 60–68

DOI
10.11598/btb.2020.27.1.1105
ISSN
1907-770X, 0215-6334
Published
2019-08-28
Publisher
SEAMEO BIOTROP
Language
en
License
creativecommons.org/licenses/by-nc-nd/4.0

Abstract

Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.

Keywords

Collaboration network of the authors

40 authors · 138 co-authorship links · showing the 40 most connected

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Co-authorOrganisationJoint articles
Asrul Sahri SiregarAffiliation unknown5
Endang HilmiAffiliation unknown5
Noprie YansahAffiliation unknown3
Tjahyo WinantoAffiliation unknown3
Sri MarnaniAffiliation unknown3
Agung SudaryonoAffiliation unknown3
Hartoyo HartoyoAffiliation unknown2
Rizqi Rizaldi HidayatAffiliation unknown2
Arif MahdianaAffiliation unknown2
Teuku JunaidiAffiliation unknown2
Rose DewiAffiliation unknown2
Yasumasa BesshoAffiliation unknown2
Taufik Budi PramonoAffiliation unknown2
Masashi KawaichiAffiliation unknown2
Iqbal Ali HusniAffiliation unknown1
Hafsarizka AkbariAffiliation unknown1
Hendrayana HendrayanaAffiliation unknown1
Tjahjo WinantoAffiliation unknown1
Petrus Hary TjahjaAffiliation unknown1
Taufan HarisanAffiliation unknown1
Hery IrawanAffiliation unknown1
Dyahruri SanjayasariAffiliation unknown1
Wahyu Adi SeptrionoAffiliation unknown1
Supono SuponoAffiliation unknown1
Sesilia Rani SamudraAffiliation unknown1
Mahardhika Nur PermatasariAffiliation unknown1
Ainulyakin Hasan ImlaniAffiliation unknown1
Hanan Hassan Alsheikh MahmoudAffiliation unknown1
Petrus Hary Tjahja SoedibjaAffiliation unknown1
Isdy SulystioAffiliation unknown1
Eko Setio WibowoAffiliation unknown1
Edy YuwonoAffiliation unknown1
Tri Nur CahyoAffiliation unknown1
Anjalli Tasha ArdiyantoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">GENETIC IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA</dc:title>
	<dc:creator xml:lang="en">Harisam, Taufan</dc:creator>
	<dc:creator xml:lang="en">Haryono, Florencius Eko Dwi</dc:creator>
	<dc:creator xml:lang="en">Marhaeni, Bintang</dc:creator>
	<dc:creator xml:lang="en">Amron, Amron</dc:creator>
	<dc:creator xml:lang="en">Prayogo, Norman Arie</dc:creator>
	<dc:creator xml:lang="en">Sukardi, Purnama</dc:creator>
	<dc:subject xml:lang="en">A. ilicifolius</dc:subject>
	<dc:subject xml:lang="en">DNA barcode</dc:subject>
	<dc:subject xml:lang="en">matK</dc:subject>
	<dc:subject xml:lang="en">rbcL</dc:subject>
	<dc:subject xml:lang="en">sea holly</dc:subject>
	<dc:description xml:lang="en">Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2019-08-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1105</dc:identifier>
	<dc:identifier>10.11598/btb.2020.27.1.1105</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020; 60 - 68</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2020.27.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1105/587</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 BIOTROPIA - The Southeast Asian Journal of Tropical Biology</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>