CHARACTERIZATION AND POLYPHASIC IDENTIFICATION OF NOVEL RHIZOBACTERIA STRAIN ISOLATED FROM SAND DUNES ECOSYSTEM

Arte Widane, Ketut; Widyasari, Annisaa; Retnaningrum, Endah

doi:10.11598/btb.2022.29.1.1584

Open access Journal SINTA 1

CHARACTERIZATION AND POLYPHASIC IDENTIFICATION OF NOVEL RHIZOBACTERIA STRAIN ISOLATED FROM SAND DUNES ECOSYSTEM

BIOTROPIA · Vol. 29 No. 1 (2022) · pp. 28–38

DOI: 10.11598/btb.2022.29.1.1584
ISSN: 1907-770X, 0215-6334
Published: 2022-04-27
Publisher: SEAMEO BIOTROP
Language: en
License: creativecommons.org/licenses/by-nc-nd/4.0

Abstract

The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this study, rhizobacteria isolated from the rhizosphere of F. cymosa were isolated to be further analyzed on their phosphate solubilizing and antagonistic properties against Fusarium oxysporum which cause the Wilt disease. The isolates of Phosphate Solubilizing Rhizobacteria (PSR) having the most potential capabilities were then polyphasically identified based on phenotypic and genotypic characters followed by 16S rDNA sequencing. The results showed that four PSR isolates (I8, I11, I12 and I24) have high phosphate dissolution indices. The highest indices were observed in isolates I11 (3.08) and I12 (3.44), respectively. Analysis of the dual plate experiments for PSR I11 and PSR I12 isolates against the growth of F. oxysporum also showed quite high inhibitory activities, i.e., isolate PSR I11 was 42.40%, while isolate PSR I12 was 42.08%. The two isolates were polyphasically identified as Burkholderia dolosa. This study clearly showed that PSR I11 and PSR I12 isolates are very potential and prospective to be used as marginal land inoculants and as providers of phosphorus. This study also showed that the isolates are useful as biocontrol agents against F. oxysporum in plants.

Keywords

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Co-author	Organisation	Joint articles
Wahyu Wilopo	Affiliation unknown	2
Laurentius Hartanto Nugroho	Affiliation unknown	2
Budi Setiadi Daryono	Affiliation unknown	2
sukirno sukirno	Affiliation unknown	1
Syarafina Ratna Putri	Affiliation unknown	1
Sri Darmawati	Affiliation unknown	1
Muhammad Evy Prastiyanto	Affiliation unknown	1
Wayan Tunas Artama	Affiliation unknown	1
Fitri Nadifah	Affiliation unknown	1
Whika Febria Dewatisari	Affiliation unknown	1
Yekti Asih Purwestri	Affiliation unknown	1
Yuli Widiyastuti	Affiliation unknown	1
Ratna Asmah Susidarti	Affiliation unknown	1
Rozana Zuhri	Affiliation unknown	1
La Ode Muhamad Syawaluddin	Affiliation unknown	1
Dewi Nursaidah Rohmah	Affiliation unknown	1
Pieter Jhon Joshua Daris	Affiliation unknown	1
Wayan Warmada	Affiliation unknown	1
Burhanuddin Ihsan	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en">CHARACTERIZATION AND POLYPHASIC IDENTIFICATION OF NOVEL RHIZOBACTERIA STRAIN ISOLATED FROM SAND DUNES ECOSYSTEM</dc:title>
	<dc:creator xml:lang="en">Arte Widane, Ketut</dc:creator>
	<dc:creator xml:lang="en">Widyasari, Annisaa</dc:creator>
	<dc:creator xml:lang="en">Retnaningrum, Endah</dc:creator>
	<dc:subject xml:lang="en">inhibitory activity</dc:subject>
	<dc:subject xml:lang="en">phosphate dissolution index</dc:subject>
	<dc:subject xml:lang="en">phosphorus</dc:subject>
	<dc:subject xml:lang="en">polyphasic identification</dc:subject>
	<dc:subject xml:lang="en">sandy soil</dc:subject>
	<dc:description xml:lang="en">The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this study, rhizobacteria isolated from the rhizosphere of F. cymosa were isolated to be further analyzed on their phosphate solubilizing and antagonistic properties against Fusarium oxysporum which cause the Wilt disease. The isolates of Phosphate Solubilizing Rhizobacteria (PSR) having the most potential capabilities were then polyphasically identified based on phenotypic and genotypic characters followed by 16S rDNA sequencing. The results showed that four PSR isolates (I8, I11, I12 and I24) have high phosphate dissolution indices. The highest indices were observed in isolates I11 (3.08) and I12 (3.44), respectively. Analysis of the dual plate experiments for PSR I11 and PSR I12 isolates against the growth of F. oxysporum also showed quite high inhibitory activities, i.e., isolate PSR I11 was 42.40%, while isolate PSR I12 was 42.08%. The two isolates were polyphasically identified as Burkholderia dolosa. This study clearly showed that PSR I11 and PSR I12 isolates are very potential and prospective to be used as marginal land inoculants and as providers of phosphorus. This study also showed that the isolates are useful as biocontrol agents against F. oxysporum in plants.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2022-04-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/1584</dc:identifier>
	<dc:identifier>10.11598/btb.2022.29.1.1584</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022; 28 - 38</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2022.29.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/1584/665</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Ketut Arte Widane, Annisaa Widyasari, Endah Retnaningrum</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>