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OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE

BIOTROPIA · Vol. 31 No. 1 (2024)

DOI
10.11598/btb.2024.31.1.2136
ISSN
1907-770X, 0215-6334
Published
2024-04-18
Publisher
SEAMEO BIOTROP
Language
en
License
creativecommons.org/licenses/by-nc-nd/4.0

Abstract

Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.

Keywords

Collaboration network of the authors

40 authors · 134 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • Lampung University
  • Jenderal Soedirman University
  • Walailak University
  • IPB University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Dyah Retno WulandariAffiliation unknown6
Sudarsono SudarsonoAffiliation unknown4
Budi WaluyoWalailak University4
Kurniawan BudiartoAffiliation unknown4
Hadi Mokhamad YusufAffiliation unknown4
Fajriyatus ShoidahAffiliation unknown4
Muhammad Asya FadillahAffiliation unknown4
Dumaris Priskila PurbaAffiliation unknown3
Alina AkhidayaAffiliation unknown3
Puji LestariJenderal Soedirman University2
Tri Muji ErmayantiAffiliation unknown2
Slamet SusantoAffiliation unknown2
Muhammad Dima Iqbal HamdaniAffiliation unknown1
Muhammad Tino FajarLampung University1
Sulastri SulastriAffiliation unknown1
Noor FaridAffiliation unknown1
Arifin Noor SugihartoAffiliation unknown1
Catur HerisonAffiliation unknown1
Surjono Hadi SutjahjoAffiliation unknown1
Rubiyo RubiyoAffiliation unknown1
Nur AjijahAffiliation unknown1
Rr. Sri HartatiAffiliation unknown1
Erick RaynaltaAffiliation unknown1
Juanita ElinaAffiliation unknown1
Mastur MasturAffiliation unknown1
I. RoostikaAffiliation unknown1
Edison HSAffiliation unknown1
Habib RijzaaniAffiliation unknown1
Hajrial AswidinnoorAffiliation unknown1
IDA HANARIDA SOMANTRIAffiliation unknown1
Irdika MansurIPB University1
Endang SuhendangAffiliation unknown1
JULIUS DWI NUGROHOAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.biotrop.org/index.php/biotropia/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE </dc:title>
	<dc:creator xml:lang="en">Nugroho, Kristianto</dc:creator>
	<dc:creator xml:lang="en">Kosmiatin, Mia</dc:creator>
	<dc:creator xml:lang="en">Santoso, Tri Joko</dc:creator>
	<dc:creator xml:lang="en">Sukma, Dewi</dc:creator>
	<dc:creator xml:lang="en">Purwito, Agus</dc:creator>
	<dc:creator xml:lang="en">Husni, Ali</dc:creator>
	<dc:creator xml:lang="en">Martasari, Chaireni</dc:creator>
	<dc:subject xml:lang="en">cotyledonary node</dc:subject>
	<dc:subject xml:lang="en">genetic transformation</dc:subject>
	<dc:subject xml:lang="en">kinetin</dc:subject>
	<dc:subject xml:lang="en">Murashige and Tucker medium</dc:subject>
	<dc:subject xml:lang="en">shoot multiplication</dc:subject>
	<dc:subject xml:lang="en">plant tissue culture</dc:subject>
	<dc:subject xml:lang="en">biotechnology</dc:subject>
	<dc:description xml:lang="en">Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.</dc:description>
	<dc:publisher xml:lang="en">SEAMEO BIOTROP</dc:publisher>
	<dc:date>2024-04-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.biotrop.org/index.php/biotropia/article/view/2136</dc:identifier>
	<dc:identifier>10.11598/btb.2024.31.1.2136</dc:identifier>
	<dc:source xml:lang="en">BIOTROPIA; Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024</dc:source>
	<dc:source>1907-770X</dc:source>
	<dc:source>0215-6334</dc:source>
	<dc:source>10.11598/btb.2024.31.1.</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.biotrop.org/index.php/biotropia/article/view/2136/786</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Kristianto Nugroho, Dr. Mia Kosmiatin, Dr. Tri Joko Santoso, Prof. Dewi Sukma, Prof. Agus Purwito, Dr. Ali Husni, Dr. Chaireni Martasari</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc-nd/4.0</dc:rights>
</oai_dc:dc>