Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Widodo, Haris Budi; Saptiwulan, Anung; Hirawan, Helmi; Prihastuti, Christiana Cahyani; Wardana, Tirta

doi:10.20473/j.djmkg.v55.i3.p137-141

Open access Journal SINTA 1

Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Dental Journal (Majalah Kedokteran Gigi) · Vol. 55 No. 3 (2022) · pp. 137–141

DOI: 10.20473/j.djmkg.v55.i3.p137-141
ISSN: 2442-9740, 1978-3728
Published: 2022-09-01
Publisher: Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
Language: eng
License: creativecommons.org/licenses/by-sa/4.0

Abstract

Background: Differences in histopathological features that describe the growth mechanism and biological behaviour of follicular and plexiform ameloblastomas are associated with benign, aggressive and destructive tumour markers. p16 has inhibitory interactions between cyclin D and CDK 4/6 to block the cell cycle and alterations related to severity. Purpose: This study intends to evaluate and determine differential expressions of p16 protein in follicular and plexiform ameloblastomas. Methods: This is a descriptive analytics study. A total of 21 specimens consisting of follicular and plexiform ameloblastomas and healthy gingiva tissues as the negative control were examined using the immunohistochemistry assay. The analysis of p16 protein expression was interpreted by immunoreactive scoring. Statistical analysis was conducted using SPSS software with the Mann–Whitney test. A p-value 0.05). Conclusion: The highest intensity of p16 protein expression was found in the plexiform type, even though it was not significantly different from the follicular type ameloblastoma.

Keywords

Collaboration network of the authors

21 authors · 55 co-authorship links · 7 across different organisations

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Jenderal Soedirman University
Nara Institute of Science and Technology
Institut Teknologi Telkom Purwokerto
Affiliation unknown

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Same organisation
Affiliation unknown
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Co-author	Organisation	Joint articles
Retno Supriyanti	Jenderal Soedirman University	4
Yogi Ramadhani	Jenderal Soedirman University	4
Ariep Soelaiman Setiadi	Jenderal Soedirman University	2
Reine Zhafirah	Affiliation unknown	2
Alifah Nur Aida	Affiliation unknown	2
Muhammad Rifqi Kurniawan	Jenderal Soedirman University	1
Arif Pujo Riyanto	Jenderal Soedirman University	1
Muhammad Syaiful Aliim	Jenderal Soedirman University	1
Muhammad Irham Akbar	Jenderal Soedirman University	1
Muhammad Alqaaf	Nara Institute of Science and Technology	1
Dody Novrial	Affiliation unknown	1
Restian Febi Andini	Affiliation unknown	1
Agi Prasetiadi	Institut Teknologi Telkom Purwokerto	1
Andre Citro Febriliyan Lanyak	Affiliation unknown	1
Muhammad Hisyam Ghani	Affiliation unknown	1
Abiyan Athallah	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/MKG/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en-US">Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma</dc:title>
	<dc:creator>Widodo, Haris Budi</dc:creator>
	<dc:creator>Saptiwulan, Anung</dc:creator>
	<dc:creator>Hirawan, Helmi</dc:creator>
	<dc:creator>Prihastuti, Christiana Cahyani</dc:creator>
	<dc:creator>Wardana, Tirta</dc:creator>
	<dc:subject xml:lang="en-US">ameloblastomas</dc:subject>
	<dc:subject xml:lang="en-US">follicular</dc:subject>
	<dc:subject xml:lang="en-US">immunohistochemistry</dc:subject>
	<dc:subject xml:lang="en-US">plexiform</dc:subject>
	<dc:subject xml:lang="en-US">p16 protein expression</dc:subject>
	<dc:description xml:lang="en-US">Background: Differences in histopathological features that describe the growth mechanism and biological behaviour of follicular and plexiform ameloblastomas are associated with benign, aggressive and destructive tumour markers. p16 has inhibitory interactions between cyclin D and CDK 4/6 to block the cell cycle and alterations related to severity. Purpose: This study intends to evaluate and determine differential expressions of p16 protein in follicular and plexiform ameloblastomas. Methods: This is a descriptive analytics study. A total of 21 specimens consisting of follicular and plexiform ameloblastomas and healthy gingiva tissues as the negative control were examined using the immunohistochemistry assay. The analysis of p16 protein expression was interpreted by immunoreactive scoring. Statistical analysis was conducted using SPSS software with the Mann–Whitney test. A p-value &amp;lt;0.05 shows the significance of the change in expression. Results: An increased expression of p16 protein was found in the follicular ameloblastoma type (2.13 ± 1.808) and the plexiform type (4.44 ± 2.506) in comparison to the negative control group (0 ± 0). The increase of p16 expression in the follicular and plexiform ameloblastomas was significant compared to the negative control group (p-value &amp;lt;0.05); however, there was no significant difference between either type of ameloblastoma (p-value &amp;gt;0.05). Conclusion: The highest intensity of p16 protein expression was found in the plexiform type, even though it was not significantly different from the follicular type ameloblastoma. </dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</dc:publisher>
	<dc:date>2022-09-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/MKG/article/view/33217</dc:identifier>
	<dc:identifier>10.20473/j.djmkg.v55.i3.p137-141</dc:identifier>
	<dc:source xml:lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol. 55 No. 3 (2022): September 2022; 137-141</dc:source>
	<dc:source>2442-9740</dc:source>
	<dc:source>1978-3728</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/33217/40048</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/MKG/article/view/33217/22216</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Dental Journal (Majalah Kedokteran Gigi)</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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